Inducing gene expression of cardiac antioxidant enzymes by dietary phenolic acids in rats

Chi Tai Yeh, Li Chien Ching, Gow Chin Yen

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

An increase in oxidative stress is suggested to be intimately involved in the pathogenesis of heart failure. Phenolic acids are widespread in plant foods; they contain important biological and pharmacological properties. This study evaluated the role of phenolic acids on the expression of antioxidant enzymes in the heart of male Sprague-Dawley rats. Gallic acid, ferulic acid and p-coumaric acid at a dosage of 100 mg kg -1 body weight significantly increased the activities of cardiac superoxide dismutase, glutathione peroxidase (GPx) and catalase (CAT) as compared with control rats (P<.05). The changes in cardiac CuZnSOD, GPx and CAT mRNA levels induced by phenolic acids were similar to those noted in the enzyme activity levels. A significant (P<.05) increase in the GSH/GSSG ratio was observed in the heart of phenolic acid-treated rats. The heart homogenates obtained from rats that were administered phenolic acids displayed significant (P<.05) increases in capacity for oxygen radical absorbance compared with control rats. Immunoblot analysis revealed the increased cardiac total level of Nrf2 in phenolic acid-treated rats. Interestingly, phenolic acid-mediated antioxidant enzyme expression was accompanied by up-regulation of heme oxygenase-1. This study demonstrates that antioxidant enzymes in rat cardiac tissue can be significantly induced by phenolic acids following oral administration.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalJournal of Nutritional Biochemistry
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Gene expression
Rats
Antioxidants
Gene Expression
Enzymes
Rat control
ferulic acid
Glutathione Peroxidase
Catalase
Gallic Acid
Edible Plants
Heme Oxygenase-1
Oxidative stress
Glutathione Disulfide
phenolic acid
Enzyme activity
Superoxide Dismutase
Oral Administration
Sprague Dawley Rats
Reactive Oxygen Species

Keywords

  • Antioxidant capacity
  • Antioxidant enzymes
  • Cardioprotection
  • Heart
  • Heme oxygenase-1

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Inducing gene expression of cardiac antioxidant enzymes by dietary phenolic acids in rats. / Yeh, Chi Tai; Ching, Li Chien; Yen, Gow Chin.

In: Journal of Nutritional Biochemistry, Vol. 20, No. 3, 03.2009, p. 163-171.

Research output: Contribution to journalArticle

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