Inducible Nitric Oxide Synthase Promoter Polymorphism, Cigarette Smoking, and Urothelial Carcinoma Risk

Cheng Huang Shen, Yuan Hung Wang, Wen Chuang Wang, Yeong Chin Jou, Hueih Shing Hsu, Hsiao Y. Hsieh, Hung Y. Chiou

Research output: Contribution to journalArticle

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Abstract

Objectives: Bladder carcinoma has a high inducible nitric oxide synthase (iNOS) content, and a highly polymorphic (CCTTT)n repeat in the iNOS promoter region has been identified. We explored whether this iNOS promoter polymorphism and cigarette smoking are associated with urothelial carcinoma (UC) risk. Methods: A total of 250 patients with pathologically confirmed UC and 250 unrelated noncancer controls were serially recruited at the Chia Yi Christian Hospital from August 2002 to May 2005. Multivariate logistic regression analysis was used to calculate the odds ratio and 95% confidence interval (CI). Results: A significantly increased UC risk was found in those who had smoked more than 30 years (odds ratio 2.4, 95% CI 1.5 to 4.2). The study subjects carrying the 12-repeat allele had a significantly increased UC risk (odds ratio 1.7, 95% CI 1.1 to 2.5). We also found the investigated polymorphism was related to clinical stage (P = 0.043). Of those who had ever smoked, those with the short/long (S/L) and long/long (L/L) genotypes (S, 9 to 11 repeats; L, 12 to 18 repeats) and the 12-repeat allele had a significantly increased UC risk of 3.5 (95% CI 1.7 to 7.3) and 4.5 (95% CI 2.2 to 8.9), respectively. Of the study subjects who had smoked longer than 30 years, those with S/L and L/L genotypes and the 12-repeat allele had significantly increased UC risks of 2.4 (95% CI 1.3 to 4.7) and 3.8 (95% CI 1.8 to 8.0), respectively. Conclusions: These findings suggest that the polymorphic (CCTTT)n repeat in the iNOS promoter region might be involved in the development of UC, especially in those who have ever smoked.

Original languageEnglish
Pages (from-to)1001-1006
Number of pages6
JournalUrology
Volume69
Issue number5
DOIs
Publication statusPublished - May 2007

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Nitric Oxide Synthase Type II
Smoking
Carcinoma
Confidence Intervals
Odds Ratio
Alleles
Genetic Promoter Regions
Genotype
Urinary Bladder
Logistic Models
Regression Analysis

ASJC Scopus subject areas

  • Urology

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Inducible Nitric Oxide Synthase Promoter Polymorphism, Cigarette Smoking, and Urothelial Carcinoma Risk. / Shen, Cheng Huang; Wang, Yuan Hung; Wang, Wen Chuang; Jou, Yeong Chin; Hsu, Hueih Shing; Hsieh, Hsiao Y.; Chiou, Hung Y.

In: Urology, Vol. 69, No. 5, 05.2007, p. 1001-1006.

Research output: Contribution to journalArticle

Shen, Cheng Huang ; Wang, Yuan Hung ; Wang, Wen Chuang ; Jou, Yeong Chin ; Hsu, Hueih Shing ; Hsieh, Hsiao Y. ; Chiou, Hung Y. / Inducible Nitric Oxide Synthase Promoter Polymorphism, Cigarette Smoking, and Urothelial Carcinoma Risk. In: Urology. 2007 ; Vol. 69, No. 5. pp. 1001-1006.
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abstract = "Objectives: Bladder carcinoma has a high inducible nitric oxide synthase (iNOS) content, and a highly polymorphic (CCTTT)n repeat in the iNOS promoter region has been identified. We explored whether this iNOS promoter polymorphism and cigarette smoking are associated with urothelial carcinoma (UC) risk. Methods: A total of 250 patients with pathologically confirmed UC and 250 unrelated noncancer controls were serially recruited at the Chia Yi Christian Hospital from August 2002 to May 2005. Multivariate logistic regression analysis was used to calculate the odds ratio and 95{\%} confidence interval (CI). Results: A significantly increased UC risk was found in those who had smoked more than 30 years (odds ratio 2.4, 95{\%} CI 1.5 to 4.2). The study subjects carrying the 12-repeat allele had a significantly increased UC risk (odds ratio 1.7, 95{\%} CI 1.1 to 2.5). We also found the investigated polymorphism was related to clinical stage (P = 0.043). Of those who had ever smoked, those with the short/long (S/L) and long/long (L/L) genotypes (S, 9 to 11 repeats; L, 12 to 18 repeats) and the 12-repeat allele had a significantly increased UC risk of 3.5 (95{\%} CI 1.7 to 7.3) and 4.5 (95{\%} CI 2.2 to 8.9), respectively. Of the study subjects who had smoked longer than 30 years, those with S/L and L/L genotypes and the 12-repeat allele had significantly increased UC risks of 2.4 (95{\%} CI 1.3 to 4.7) and 3.8 (95{\%} CI 1.8 to 8.0), respectively. Conclusions: These findings suggest that the polymorphic (CCTTT)n repeat in the iNOS promoter region might be involved in the development of UC, especially in those who have ever smoked.",
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AU - Shen, Cheng Huang

AU - Wang, Yuan Hung

AU - Wang, Wen Chuang

AU - Jou, Yeong Chin

AU - Hsu, Hueih Shing

AU - Hsieh, Hsiao Y.

AU - Chiou, Hung Y.

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N2 - Objectives: Bladder carcinoma has a high inducible nitric oxide synthase (iNOS) content, and a highly polymorphic (CCTTT)n repeat in the iNOS promoter region has been identified. We explored whether this iNOS promoter polymorphism and cigarette smoking are associated with urothelial carcinoma (UC) risk. Methods: A total of 250 patients with pathologically confirmed UC and 250 unrelated noncancer controls were serially recruited at the Chia Yi Christian Hospital from August 2002 to May 2005. Multivariate logistic regression analysis was used to calculate the odds ratio and 95% confidence interval (CI). Results: A significantly increased UC risk was found in those who had smoked more than 30 years (odds ratio 2.4, 95% CI 1.5 to 4.2). The study subjects carrying the 12-repeat allele had a significantly increased UC risk (odds ratio 1.7, 95% CI 1.1 to 2.5). We also found the investigated polymorphism was related to clinical stage (P = 0.043). Of those who had ever smoked, those with the short/long (S/L) and long/long (L/L) genotypes (S, 9 to 11 repeats; L, 12 to 18 repeats) and the 12-repeat allele had a significantly increased UC risk of 3.5 (95% CI 1.7 to 7.3) and 4.5 (95% CI 2.2 to 8.9), respectively. Of the study subjects who had smoked longer than 30 years, those with S/L and L/L genotypes and the 12-repeat allele had significantly increased UC risks of 2.4 (95% CI 1.3 to 4.7) and 3.8 (95% CI 1.8 to 8.0), respectively. Conclusions: These findings suggest that the polymorphic (CCTTT)n repeat in the iNOS promoter region might be involved in the development of UC, especially in those who have ever smoked.

AB - Objectives: Bladder carcinoma has a high inducible nitric oxide synthase (iNOS) content, and a highly polymorphic (CCTTT)n repeat in the iNOS promoter region has been identified. We explored whether this iNOS promoter polymorphism and cigarette smoking are associated with urothelial carcinoma (UC) risk. Methods: A total of 250 patients with pathologically confirmed UC and 250 unrelated noncancer controls were serially recruited at the Chia Yi Christian Hospital from August 2002 to May 2005. Multivariate logistic regression analysis was used to calculate the odds ratio and 95% confidence interval (CI). Results: A significantly increased UC risk was found in those who had smoked more than 30 years (odds ratio 2.4, 95% CI 1.5 to 4.2). The study subjects carrying the 12-repeat allele had a significantly increased UC risk (odds ratio 1.7, 95% CI 1.1 to 2.5). We also found the investigated polymorphism was related to clinical stage (P = 0.043). Of those who had ever smoked, those with the short/long (S/L) and long/long (L/L) genotypes (S, 9 to 11 repeats; L, 12 to 18 repeats) and the 12-repeat allele had a significantly increased UC risk of 3.5 (95% CI 1.7 to 7.3) and 4.5 (95% CI 2.2 to 8.9), respectively. Of the study subjects who had smoked longer than 30 years, those with S/L and L/L genotypes and the 12-repeat allele had significantly increased UC risks of 2.4 (95% CI 1.3 to 4.7) and 3.8 (95% CI 1.8 to 8.0), respectively. Conclusions: These findings suggest that the polymorphic (CCTTT)n repeat in the iNOS promoter region might be involved in the development of UC, especially in those who have ever smoked.

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