Inducible nitric oxide synthase inhibition influenced granuloma formation with suppressed collagen expression in myositis caused by Toxocara canis in mice

Su M. Lin, Chien W. Liao, Yun H. Lin, Chin Cheng Lee, Ting Chang Kao, Chia Kwung Fan

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Abstract

The role of nitric oxide (NO) in granuloma pathology is largely unclear to date. We investigated the role of NO in fibrotic granuloma development in the musculature of mice infected with Toxocara canis from 1 day (dpi) to 8 weeks post-infection (wpi) using the NO synthase (NOS) inhibitors, L-NIL (l-N 6-1-iminoethyl lysine). In infected mice, elevated serum NO concentrations were seen at 1 dpi (204.1±0.2 μM) and 1 wpi (145.1±0.2 μM); it declined drastically from 4 wpi onwards (57.0±0.1 μM). In L-NIL-treated mice, the NO concentration was drastically reduced from 15% during 1 wpi; thereafter, it was restored to almost half that in infected mice. Inducible NOS expression was enhanced in infected and L-NIL-treated mice at 4 wpi but declined at 8 wpi as assessed by immunohistochemistry. L-NIL treatment resulted in large, irregularly shaped granulomas with suppressed collagen contents at 4 wpi but not at 8 wpi. The suppressed collagen contents might have been related to decreased serum NO and Th2-type cytokine of interleukin-4 but not Th1-type cytokine of interferon-γ expression.

Original languageEnglish
Pages (from-to)577-585
Number of pages9
JournalParasitology Research
Volume102
Issue number4
DOIs
Publication statusPublished - Mar 2008

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Toxocara canis
myositis
Myositis
granuloma
Nitric Oxide Synthase Type II
Granuloma
collagen
Collagen
nitric oxide
mice
Nitric Oxide
Infection
infection
lysine
Nitric Oxide Synthase
cytokines
Cytokines
inducible nitric oxide synthase
interferons
interleukin-4

ASJC Scopus subject areas

  • Parasitology

Cite this

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title = "Inducible nitric oxide synthase inhibition influenced granuloma formation with suppressed collagen expression in myositis caused by Toxocara canis in mice",
abstract = "The role of nitric oxide (NO) in granuloma pathology is largely unclear to date. We investigated the role of NO in fibrotic granuloma development in the musculature of mice infected with Toxocara canis from 1 day (dpi) to 8 weeks post-infection (wpi) using the NO synthase (NOS) inhibitors, L-NIL (l-N 6-1-iminoethyl lysine). In infected mice, elevated serum NO concentrations were seen at 1 dpi (204.1±0.2 μM) and 1 wpi (145.1±0.2 μM); it declined drastically from 4 wpi onwards (57.0±0.1 μM). In L-NIL-treated mice, the NO concentration was drastically reduced from 15{\%} during 1 wpi; thereafter, it was restored to almost half that in infected mice. Inducible NOS expression was enhanced in infected and L-NIL-treated mice at 4 wpi but declined at 8 wpi as assessed by immunohistochemistry. L-NIL treatment resulted in large, irregularly shaped granulomas with suppressed collagen contents at 4 wpi but not at 8 wpi. The suppressed collagen contents might have been related to decreased serum NO and Th2-type cytokine of interleukin-4 but not Th1-type cytokine of interferon-γ expression.",
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AU - Liao, Chien W.

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AU - Lee, Chin Cheng

AU - Kao, Ting Chang

AU - Fan, Chia Kwung

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AB - The role of nitric oxide (NO) in granuloma pathology is largely unclear to date. We investigated the role of NO in fibrotic granuloma development in the musculature of mice infected with Toxocara canis from 1 day (dpi) to 8 weeks post-infection (wpi) using the NO synthase (NOS) inhibitors, L-NIL (l-N 6-1-iminoethyl lysine). In infected mice, elevated serum NO concentrations were seen at 1 dpi (204.1±0.2 μM) and 1 wpi (145.1±0.2 μM); it declined drastically from 4 wpi onwards (57.0±0.1 μM). In L-NIL-treated mice, the NO concentration was drastically reduced from 15% during 1 wpi; thereafter, it was restored to almost half that in infected mice. Inducible NOS expression was enhanced in infected and L-NIL-treated mice at 4 wpi but declined at 8 wpi as assessed by immunohistochemistry. L-NIL treatment resulted in large, irregularly shaped granulomas with suppressed collagen contents at 4 wpi but not at 8 wpi. The suppressed collagen contents might have been related to decreased serum NO and Th2-type cytokine of interleukin-4 but not Th1-type cytokine of interferon-γ expression.

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