Inducible nitric oxide synthase and cyclooxygenase-2 participate in anti-inflammatory activity of imperatorin from Glehnia littoralis

Guan Jhong Huang, Jeng Shyan Deng, Jung Chun Liao, Wen Chi Hou, Sheng Yang Wang, Ping Jyun Sung, Yueh Hsiung Kuo

Research output: Contribution to journalArticle

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Abstract

In this study, we have investigated the anti-inflammatory effects of imperatorin, a compound isolated from the roots of Glehnia littoralis, using a lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) in vitro and a carrageenan (Carr)-induced mouse paw edema model in vivo. When RAW264.7 macrophages were treated with imperatorin together with LPS, a significant concentration-dependent inhibition of NO production was detected. Western blotting revealed that imperatorin blocked the protein expression of iNOS and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 macrophages significantly. In the anti-inflammatory test, imperatorin decreased the paw edema at 4 and 5 h after Carr administration and increased the activities of catalase, superoxide dismutase, and glutathione peroxidase in paw edema. We also demonstrated that imperatorin significantly attenuated the malondialdehyde level in the edema paw at the fifth hour after Carr injection. Imperatorin decreased the NO and tumor necrosis factor and prostaglandin E2 levels on serum at 5 h after Carr injection. Western blotting revealed that imperatorin decreased Carr-induced iNOS and COX-2 expressions at 5 h in edema paw. An intraperitoneal injection treatment with imperatorin also diminished neutrophil infiltration into sites of inflammation as did indomethacin. The results suggested that imperatorin had anti-inflammatory effects in LPS-stimulated RAW 264.7 cells and Carr-injected mice, respectively. In addition, inhibition of elevated iNOS and COX-2 protein expression as well as neutrophil infiltration of Carr-injected paws may be involved in the beneficial effects of imperatorin.

Original languageEnglish
Pages (from-to)1673-1681
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number7
DOIs
Publication statusPublished - Feb 22 2012

Fingerprint

Apiaceae
carrageenan
Nitric Oxide Synthase Type II
prostaglandin synthase
Cyclooxygenase 2
anti-inflammatory activity
Carrageenan
Anti-Inflammatory Agents
edema
lipopolysaccharides
Edema
Lipopolysaccharides
macrophages
Macrophages
neutrophils
mice
Neutrophil Infiltration
Western blotting
protein synthesis
Infiltration

Keywords

  • anti-inflammation
  • Chinese herb
  • imperatorin
  • NO
  • TNF-α

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Inducible nitric oxide synthase and cyclooxygenase-2 participate in anti-inflammatory activity of imperatorin from Glehnia littoralis. / Huang, Guan Jhong; Deng, Jeng Shyan; Liao, Jung Chun; Hou, Wen Chi; Wang, Sheng Yang; Sung, Ping Jyun; Kuo, Yueh Hsiung.

In: Journal of Agricultural and Food Chemistry, Vol. 60, No. 7, 22.02.2012, p. 1673-1681.

Research output: Contribution to journalArticle

Huang, Guan Jhong ; Deng, Jeng Shyan ; Liao, Jung Chun ; Hou, Wen Chi ; Wang, Sheng Yang ; Sung, Ping Jyun ; Kuo, Yueh Hsiung. / Inducible nitric oxide synthase and cyclooxygenase-2 participate in anti-inflammatory activity of imperatorin from Glehnia littoralis. In: Journal of Agricultural and Food Chemistry. 2012 ; Vol. 60, No. 7. pp. 1673-1681.
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AU - Kuo, Yueh Hsiung

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