Inducibility of abnormal automaticity and triggered activity in myocardial sleeves of canine pulmonary veins

Tsui Min Wang, Hsiang Ning Luk, Joen Rong Sheu, Hsiao Ping Wu, Chern En Chiang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: To study the cellular mechanisms governing cardiac atrial arrhythmias initiated by ectopic focus (or foci) from pulmonary veins (PVs). Methods: In the present in vitro study, we applied the conventional microelectrode technique to record intracellular action potentials in PV sleeves from dogs. Results: In 80 normal healthy dogs, all action potentials recorded in cardiomyocytes from PV sleeves were fast-response. The pharmacological responses to quinidine, nisoldipine, d-sotalol, 4-aminopyridine, isoproterenol, acetylcholine, and adenosine were characteristic of those in atrial cells. Diastolic depolarization and spontaneous activity could be induced by 1 mmol/L Ba2+ in all the 22 PV specimens being tested, but only in 3 of 11 of left atrial specimens (p2+, the diastolic slope was only slightly affected by Ni2+ (500 μmol/L), but was significantly suppressed by Cd2+ (200 μmol/L). Ryanodine (2 μmol/L) caused a transient increase, followed by a marked decrease of Ba2+-induced spontaneous activity. Isoproterenol shortened and acetylcholine prolonged the cycle length of the Ba 2+-induced automatic activity. In the presence of isoproterenol, washout of acetylcholine induced a rebound phenomenon, which triggered a short period of spontaneous activity. The results suggest an important role of intracellular cytoplasmic Ca2+ loading. Under conditions that mimic ischemia/hypoxia, the resting membrane potential depolarized, upstroke of the action potential became depressed and the action potential duration shortened. In the presence of isoproterenol and elevated external K+, spontaneous activity was generated. Conclusions: These findings indicate a lack of arrhythmogenic activity in normal healthy PV sleeves. Abnormal automaticity and triggered activity occurred exclusively under simulated pathologic conditions. Ba2+-induced automaticity was more easily induced in PV than in the left atrium. The same conditions might also favor the genesis of reentry in the in vivo condition.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalInternational Journal of Cardiology
Volume104
Issue number1
DOIs
Publication statusPublished - Sep 15 2005

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Pulmonary Veins
Canidae
Isoproterenol
Action Potentials
Acetylcholine
Nisoldipine
Dogs
Sotalol
Ryanodine
4-Aminopyridine
Quinidine
Microelectrodes
Heart Atria
Cardiac Myocytes
Membrane Potentials
Adenosine
Cardiac Arrhythmias
Ischemia
Pharmacology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Inducibility of abnormal automaticity and triggered activity in myocardial sleeves of canine pulmonary veins. / Wang, Tsui Min; Luk, Hsiang Ning; Sheu, Joen Rong; Wu, Hsiao Ping; Chiang, Chern En.

In: International Journal of Cardiology, Vol. 104, No. 1, 15.09.2005, p. 59-66.

Research output: Contribution to journalArticle

Wang, Tsui Min ; Luk, Hsiang Ning ; Sheu, Joen Rong ; Wu, Hsiao Ping ; Chiang, Chern En. / Inducibility of abnormal automaticity and triggered activity in myocardial sleeves of canine pulmonary veins. In: International Journal of Cardiology. 2005 ; Vol. 104, No. 1. pp. 59-66.
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abstract = "Background: To study the cellular mechanisms governing cardiac atrial arrhythmias initiated by ectopic focus (or foci) from pulmonary veins (PVs). Methods: In the present in vitro study, we applied the conventional microelectrode technique to record intracellular action potentials in PV sleeves from dogs. Results: In 80 normal healthy dogs, all action potentials recorded in cardiomyocytes from PV sleeves were fast-response. The pharmacological responses to quinidine, nisoldipine, d-sotalol, 4-aminopyridine, isoproterenol, acetylcholine, and adenosine were characteristic of those in atrial cells. Diastolic depolarization and spontaneous activity could be induced by 1 mmol/L Ba2+ in all the 22 PV specimens being tested, but only in 3 of 11 of left atrial specimens (p2+, the diastolic slope was only slightly affected by Ni2+ (500 μmol/L), but was significantly suppressed by Cd2+ (200 μmol/L). Ryanodine (2 μmol/L) caused a transient increase, followed by a marked decrease of Ba2+-induced spontaneous activity. Isoproterenol shortened and acetylcholine prolonged the cycle length of the Ba 2+-induced automatic activity. In the presence of isoproterenol, washout of acetylcholine induced a rebound phenomenon, which triggered a short period of spontaneous activity. The results suggest an important role of intracellular cytoplasmic Ca2+ loading. Under conditions that mimic ischemia/hypoxia, the resting membrane potential depolarized, upstroke of the action potential became depressed and the action potential duration shortened. In the presence of isoproterenol and elevated external K+, spontaneous activity was generated. Conclusions: These findings indicate a lack of arrhythmogenic activity in normal healthy PV sleeves. Abnormal automaticity and triggered activity occurred exclusively under simulated pathologic conditions. Ba2+-induced automaticity was more easily induced in PV than in the left atrium. The same conditions might also favor the genesis of reentry in the in vivo condition.",
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