Indirect inhibition of generation of murine lymphokine-activated killer cell activity in splenocyte cultures by interferon-gamma

T. Y. Chao, H. Ohnishi, T. M. Chu

Research output: Contribution to journalArticle

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Abstract

The effect of mouse recombinant interferon-gamma (rIFN-γ) on murine lymphokine-activated killer (LAK) cell activity was investigated using natural killer (NK)-resistant, spontaneously developed, weakly immunogenic and highly tumourigenic, syngeneic murine mammary adenocarcinoma, JC, mimicking that of human disease, as the target. Murine YAC-1 also was used as a target cell line. rIFN-γ, when used in combination with recombinant interleukin-2 (rIL-2), was shown to exhibit a suppressed effect on LAK cell activity generated from BALB/c mouse splenocytes, compared to that with rIL-2 alone. The decrease in LAK cell activity was rIFN-γ dose-dependent. Kinetic study revealed that this inhibitory effect was demonstrated when rIFN-γ was added to the medium at the early phase of rIL-2 culture. The inhibitory effect on LAK cell generation by rIFN-γ was completely abrogated when the nylon-wool-treated non-adherent 'macrophage-free' splenocytes were incubated with rIL-2 and rIFN-γ. These results indicated that the LAK cell activity generated from murine splenocytes cultured with rIL-2 could be depressed by rIFN-γ, and that the macrophages may be involved as mediators.

Original languageEnglish
Pages (from-to)116-120
Number of pages5
JournalImmunology
Volume70
Issue number1
Publication statusPublished - 1990
Externally publishedYes

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Lymphokine-Activated Killer Cells
Interferon-gamma
Interleukin-2
Macrophages
Wool
Nylons
Adenocarcinoma
Breast
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Indirect inhibition of generation of murine lymphokine-activated killer cell activity in splenocyte cultures by interferon-gamma. / Chao, T. Y.; Ohnishi, H.; Chu, T. M.

In: Immunology, Vol. 70, No. 1, 1990, p. 116-120.

Research output: Contribution to journalArticle

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