Increasing expression of extracellular matrix metalloprotease inducer in renal cell carcinoma

Tissue microarray analysis of immunostaining score with clinicopathological parameters

Jong Shiaw Jin, Dar Shih Hsieh, Yeh Feng Lin, Jia Yi Wang, Lai Fa Sheu, Wei Hwa Lee

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32 Citations (Scopus)

Abstract

Aim: Renal tumor cell invasion is responsible for both local tissue destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell-derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesized that EMMPRIN and matrix metalloproteinase-9 (MMP-9) are over-expressed in renal cell carcinoma. Methods: Immunohistochemical analysis of EMMPRIN and MMP-9 was performed in tissue microarrays of 79 renal cell carcinomas including 12 cases of chromophobe renal cell carcinoma (ChRCC), 53 cases of clear cell renal cell carcinoma (CRCC), 8 cases of papillary renal cell carcinoma (PRCC), and 6 cases of carcinoma of the collecting ducts of Bellini (CoRCC). Results: All renal cell carcinomas showed significant immunohistochemical expression of EMMPRIN. The EMMPRIN score in ChRCC (321 ± 21) was significantly higher than in other histological subtypes of RCC (166 ± 19 for CRCC; 276 ± 24 for PRCC; 98 ± 17 for CoRCC). MMP-9 was mainly expressed in tumor stromal cells and not in non-cancerous fibrovascular regions. The percent positive staining of MMP-9 at the invasive front of tumor cells was significantly higher in CRCC than in ChRCC, PRCC, or CoRCC. Higher EMMPRIN scores in CRCC were associated with shorter survival time, and correlated with higher T staging and nuclear grading. Conclusions: Our findings demonstrate for the first time that EMMPRIN is over-expressed in renal cell carcinomas. Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with CRCC.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalInternational Journal of Urology
Volume13
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

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Tissue Array Analysis
Metalloproteases
Renal Cell Carcinoma
Extracellular Matrix
Matrix Metalloproteinase 9
Neoplasms

Keywords

  • Clear cell subtype
  • EMMPRIN
  • Extracellular matrix metalloprotease inducer
  • Matrix metalloprotease-9
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Urology

Cite this

@article{ab7db89e2eef471d9b6fa563a06e3bdb,
title = "Increasing expression of extracellular matrix metalloprotease inducer in renal cell carcinoma: Tissue microarray analysis of immunostaining score with clinicopathological parameters",
abstract = "Aim: Renal tumor cell invasion is responsible for both local tissue destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell-derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesized that EMMPRIN and matrix metalloproteinase-9 (MMP-9) are over-expressed in renal cell carcinoma. Methods: Immunohistochemical analysis of EMMPRIN and MMP-9 was performed in tissue microarrays of 79 renal cell carcinomas including 12 cases of chromophobe renal cell carcinoma (ChRCC), 53 cases of clear cell renal cell carcinoma (CRCC), 8 cases of papillary renal cell carcinoma (PRCC), and 6 cases of carcinoma of the collecting ducts of Bellini (CoRCC). Results: All renal cell carcinomas showed significant immunohistochemical expression of EMMPRIN. The EMMPRIN score in ChRCC (321 ± 21) was significantly higher than in other histological subtypes of RCC (166 ± 19 for CRCC; 276 ± 24 for PRCC; 98 ± 17 for CoRCC). MMP-9 was mainly expressed in tumor stromal cells and not in non-cancerous fibrovascular regions. The percent positive staining of MMP-9 at the invasive front of tumor cells was significantly higher in CRCC than in ChRCC, PRCC, or CoRCC. Higher EMMPRIN scores in CRCC were associated with shorter survival time, and correlated with higher T staging and nuclear grading. Conclusions: Our findings demonstrate for the first time that EMMPRIN is over-expressed in renal cell carcinomas. Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with CRCC.",
keywords = "Clear cell subtype, EMMPRIN, Extracellular matrix metalloprotease inducer, Matrix metalloprotease-9, Renal cell carcinoma",
author = "Jin, {Jong Shiaw} and Hsieh, {Dar Shih} and Lin, {Yeh Feng} and Wang, {Jia Yi} and Sheu, {Lai Fa} and Lee, {Wei Hwa}",
year = "2006",
month = "5",
doi = "10.1111/j.1442-2042.2006.01353.x",
language = "English",
volume = "13",
pages = "573--580",
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issn = "0919-8172",
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TY - JOUR

T1 - Increasing expression of extracellular matrix metalloprotease inducer in renal cell carcinoma

T2 - Tissue microarray analysis of immunostaining score with clinicopathological parameters

AU - Jin, Jong Shiaw

AU - Hsieh, Dar Shih

AU - Lin, Yeh Feng

AU - Wang, Jia Yi

AU - Sheu, Lai Fa

AU - Lee, Wei Hwa

PY - 2006/5

Y1 - 2006/5

N2 - Aim: Renal tumor cell invasion is responsible for both local tissue destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell-derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesized that EMMPRIN and matrix metalloproteinase-9 (MMP-9) are over-expressed in renal cell carcinoma. Methods: Immunohistochemical analysis of EMMPRIN and MMP-9 was performed in tissue microarrays of 79 renal cell carcinomas including 12 cases of chromophobe renal cell carcinoma (ChRCC), 53 cases of clear cell renal cell carcinoma (CRCC), 8 cases of papillary renal cell carcinoma (PRCC), and 6 cases of carcinoma of the collecting ducts of Bellini (CoRCC). Results: All renal cell carcinomas showed significant immunohistochemical expression of EMMPRIN. The EMMPRIN score in ChRCC (321 ± 21) was significantly higher than in other histological subtypes of RCC (166 ± 19 for CRCC; 276 ± 24 for PRCC; 98 ± 17 for CoRCC). MMP-9 was mainly expressed in tumor stromal cells and not in non-cancerous fibrovascular regions. The percent positive staining of MMP-9 at the invasive front of tumor cells was significantly higher in CRCC than in ChRCC, PRCC, or CoRCC. Higher EMMPRIN scores in CRCC were associated with shorter survival time, and correlated with higher T staging and nuclear grading. Conclusions: Our findings demonstrate for the first time that EMMPRIN is over-expressed in renal cell carcinomas. Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with CRCC.

AB - Aim: Renal tumor cell invasion is responsible for both local tissue destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell-derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesized that EMMPRIN and matrix metalloproteinase-9 (MMP-9) are over-expressed in renal cell carcinoma. Methods: Immunohistochemical analysis of EMMPRIN and MMP-9 was performed in tissue microarrays of 79 renal cell carcinomas including 12 cases of chromophobe renal cell carcinoma (ChRCC), 53 cases of clear cell renal cell carcinoma (CRCC), 8 cases of papillary renal cell carcinoma (PRCC), and 6 cases of carcinoma of the collecting ducts of Bellini (CoRCC). Results: All renal cell carcinomas showed significant immunohistochemical expression of EMMPRIN. The EMMPRIN score in ChRCC (321 ± 21) was significantly higher than in other histological subtypes of RCC (166 ± 19 for CRCC; 276 ± 24 for PRCC; 98 ± 17 for CoRCC). MMP-9 was mainly expressed in tumor stromal cells and not in non-cancerous fibrovascular regions. The percent positive staining of MMP-9 at the invasive front of tumor cells was significantly higher in CRCC than in ChRCC, PRCC, or CoRCC. Higher EMMPRIN scores in CRCC were associated with shorter survival time, and correlated with higher T staging and nuclear grading. Conclusions: Our findings demonstrate for the first time that EMMPRIN is over-expressed in renal cell carcinomas. Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with CRCC.

KW - Clear cell subtype

KW - EMMPRIN

KW - Extracellular matrix metalloprotease inducer

KW - Matrix metalloprotease-9

KW - Renal cell carcinoma

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DO - 10.1111/j.1442-2042.2006.01353.x

M3 - Article

VL - 13

SP - 573

EP - 580

JO - International Journal of Urology

JF - International Journal of Urology

SN - 0919-8172

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