Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma: The role of nitric oxide

Chun Hua Wang, Wen Yih Hsieh, Lee Yung Shih, Horng Chyuan Lin, Chien Ying Liu, Kian Fan Chung, Han Pin Kuo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Asthma exacerbation is associated with increased numbers of circulating CD34+ progenitor cells, which may migrate to airways and develop into mature cells under the effects of cytokines and hematopoietic factors. Nitric oxide (NO) generation is enhanced in asthma and is known to suppress human hematopoiesis. Objectives: We studied circulating progenitor cells in the blood of patients with varying severity of asthma and examined the contribution of NO to their proliferation into eosinophil-forming colonies ex vivo. Methods: With use of multiparameter flow cytometric analyses, the cell numbers and intracellular inducible NO synthase (iNOS) immunoreactivity of circulating CD34+ cells in peripheral blood was measured. The serum level of GM-CSF or IL-5 was also determined. The colonies grown from progenitor cells were cultured in methylcellulose either in the presence or absence of growth factors, including GM-CSF, stem cell factor, and IL-3. Results: A significantly greater number of circulating CD34+ cells increased together with higher intracellular iNOS immunoreactivity in moderate asthmatics compared with mild intermittent asthmatics and healthy subjects. There was no significant difference in iNOS immunoreactivities or CD34+ progenitor cell numbers between healthy subjects and those with mild intermittent asthma. Serum levels of GM-CSF or IL-5 were significantly higher in all asthmatics compared with healthy subjects and correlated with circulating CD34+ cells. A greater number of colonies was grown either in the presence or absence of growth factors with a higher percentage of cells of eosinophil lineage in asthmatics than in health subjects. NG-nitro-L-arginine methyl ester potentiated and sodium nitroprusside inhibited the colony growth in both asthmatic and healthy subjects without a significant change in the percentage of eosinophil lineage. Conclusions: The production of NO from progenitor cells or other circulating cells may act in an autocrine or paracrine fashion to regulate progenitor cell growth and colony forma-tion. However, this is not sufficient to control the increased proliferation of progenitor cells observed in asthma.

Original languageEnglish
Pages (from-to)803-810
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume104
Issue number4 I
Publication statusPublished - Dec 1 1999
Externally publishedYes

Fingerprint

Nitric Oxide
Stem Cells
Asthma
Cell Proliferation
Healthy Volunteers
Granulocyte-Macrophage Colony-Stimulating Factor
Eosinophils
Interleukin-5
Nitric Oxide Synthase
Intercellular Signaling Peptides and Proteins
Cell Count
Methylcellulose
Stem Cell Factor
Interleukin-3
NG-Nitroarginine Methyl Ester
Hematopoiesis
Nitroprusside
Nitric Oxide Synthase Type II
Cell Lineage
Growth

Keywords

  • Asthma
  • Cytokines
  • Nitric oxide
  • Nitric oxide synthase
  • Progenitor cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma : The role of nitric oxide. / Wang, Chun Hua; Hsieh, Wen Yih; Shih, Lee Yung; Lin, Horng Chyuan; Liu, Chien Ying; Chung, Kian Fan; Kuo, Han Pin.

In: Journal of Allergy and Clinical Immunology, Vol. 104, No. 4 I, 01.12.1999, p. 803-810.

Research output: Contribution to journalArticle

Wang, Chun Hua ; Hsieh, Wen Yih ; Shih, Lee Yung ; Lin, Horng Chyuan ; Liu, Chien Ying ; Chung, Kian Fan ; Kuo, Han Pin. / Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma : The role of nitric oxide. In: Journal of Allergy and Clinical Immunology. 1999 ; Vol. 104, No. 4 I. pp. 803-810.
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T1 - Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma

T2 - The role of nitric oxide

AU - Wang, Chun Hua

AU - Hsieh, Wen Yih

AU - Shih, Lee Yung

AU - Lin, Horng Chyuan

AU - Liu, Chien Ying

AU - Chung, Kian Fan

AU - Kuo, Han Pin

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Background: Asthma exacerbation is associated with increased numbers of circulating CD34+ progenitor cells, which may migrate to airways and develop into mature cells under the effects of cytokines and hematopoietic factors. Nitric oxide (NO) generation is enhanced in asthma and is known to suppress human hematopoiesis. Objectives: We studied circulating progenitor cells in the blood of patients with varying severity of asthma and examined the contribution of NO to their proliferation into eosinophil-forming colonies ex vivo. Methods: With use of multiparameter flow cytometric analyses, the cell numbers and intracellular inducible NO synthase (iNOS) immunoreactivity of circulating CD34+ cells in peripheral blood was measured. The serum level of GM-CSF or IL-5 was also determined. The colonies grown from progenitor cells were cultured in methylcellulose either in the presence or absence of growth factors, including GM-CSF, stem cell factor, and IL-3. Results: A significantly greater number of circulating CD34+ cells increased together with higher intracellular iNOS immunoreactivity in moderate asthmatics compared with mild intermittent asthmatics and healthy subjects. There was no significant difference in iNOS immunoreactivities or CD34+ progenitor cell numbers between healthy subjects and those with mild intermittent asthma. Serum levels of GM-CSF or IL-5 were significantly higher in all asthmatics compared with healthy subjects and correlated with circulating CD34+ cells. A greater number of colonies was grown either in the presence or absence of growth factors with a higher percentage of cells of eosinophil lineage in asthmatics than in health subjects. NG-nitro-L-arginine methyl ester potentiated and sodium nitroprusside inhibited the colony growth in both asthmatic and healthy subjects without a significant change in the percentage of eosinophil lineage. Conclusions: The production of NO from progenitor cells or other circulating cells may act in an autocrine or paracrine fashion to regulate progenitor cell growth and colony forma-tion. However, this is not sufficient to control the increased proliferation of progenitor cells observed in asthma.

AB - Background: Asthma exacerbation is associated with increased numbers of circulating CD34+ progenitor cells, which may migrate to airways and develop into mature cells under the effects of cytokines and hematopoietic factors. Nitric oxide (NO) generation is enhanced in asthma and is known to suppress human hematopoiesis. Objectives: We studied circulating progenitor cells in the blood of patients with varying severity of asthma and examined the contribution of NO to their proliferation into eosinophil-forming colonies ex vivo. Methods: With use of multiparameter flow cytometric analyses, the cell numbers and intracellular inducible NO synthase (iNOS) immunoreactivity of circulating CD34+ cells in peripheral blood was measured. The serum level of GM-CSF or IL-5 was also determined. The colonies grown from progenitor cells were cultured in methylcellulose either in the presence or absence of growth factors, including GM-CSF, stem cell factor, and IL-3. Results: A significantly greater number of circulating CD34+ cells increased together with higher intracellular iNOS immunoreactivity in moderate asthmatics compared with mild intermittent asthmatics and healthy subjects. There was no significant difference in iNOS immunoreactivities or CD34+ progenitor cell numbers between healthy subjects and those with mild intermittent asthma. Serum levels of GM-CSF or IL-5 were significantly higher in all asthmatics compared with healthy subjects and correlated with circulating CD34+ cells. A greater number of colonies was grown either in the presence or absence of growth factors with a higher percentage of cells of eosinophil lineage in asthmatics than in health subjects. NG-nitro-L-arginine methyl ester potentiated and sodium nitroprusside inhibited the colony growth in both asthmatic and healthy subjects without a significant change in the percentage of eosinophil lineage. Conclusions: The production of NO from progenitor cells or other circulating cells may act in an autocrine or paracrine fashion to regulate progenitor cell growth and colony forma-tion. However, this is not sufficient to control the increased proliferation of progenitor cells observed in asthma.

KW - Asthma

KW - Cytokines

KW - Nitric oxide

KW - Nitric oxide synthase

KW - Progenitor cells

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