Increased production off hydrogen peroxide and expression of CD11b/CD18 on alveolar macrophages in patients with active pulmonary tuberculosis

H. P. Kuo, T. C. Ho, C. H. Wang, C. T. Yu, H. C. Lin

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Setting: Alveolar macrophages (AM) are important in host defense against Mycobacterium tuberculosis (TB). β2-integrins, especially CD11a/CD18 and CD11b/CD18, are implicated in leukocyte migration, antigen presentation, phagocytosis, and production of reactive oxygen species Objective: To explore the functional relevance of β2-integrin expression to intracellular H2O2 capacity of AM in TB patients. Design: In a prospective study, AM retrieved from 18 active pulmonary TB patients and 18 normal subjects were assessed for β2-integrin expression and intracellular H2O2 metabolism capacity by loading with anti-CD11a/CD18, anti-CD11b/CD18 monoclonal antibodies and 2',7' dichlorofluorescein diacetate (DCFH-DA) respectively, and analyzed by flow cytometry. AM from 8 normal subjects were stimulated with tumor necrosis factor-alpha (TNF-α, 105 units/ml) to examine the relationship between H2O2 production and CD11b/CD18 expression. Results: The magnitude of DCFH oxidation and CD11b/CD18 expression of AM was higher in TB patients than in normal subjects. The CD11b/CD18 expression was related to the magnitude of DCFH oxidation, but not to lymphocyte numbers or subpopulations (CD4, CD8, CD25). Stimulation of AM with TNF-α increased H2O2 production and CD11b/CD18 expression. Pretreatment with CD11b/CD18 monoclonal antibodies inhibited TNF-α-induced H2O2. Conclusion: AM in TB patients possessed a higher capacity of oxidant metabolism. The increased CD11b/CD18 expression may be related to the increased respiratory burst response in AM against mycobacterial invasion.

Original languageEnglish
Pages (from-to)468-475
Number of pages8
JournalTubercle and Lung Disease
Volume77
Issue number5
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Increased production off hydrogen peroxide and expression of CD11b/CD18 on alveolar macrophages in patients with active pulmonary tuberculosis'. Together they form a unique fingerprint.

Cite this