Increased production off hydrogen peroxide and expression of CD11b/CD18 on alveolar macrophages in patients with active pulmonary tuberculosis

H. P. Kuo, T. C. Ho, C. H. Wang, C. T. Yu, H. C. Lin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Setting: Alveolar macrophages (AM) are important in host defense against Mycobacterium tuberculosis (TB). β2-integrins, especially CD11a/CD18 and CD11b/CD18, are implicated in leukocyte migration, antigen presentation, phagocytosis, and production of reactive oxygen species Objective: To explore the functional relevance of β2-integrin expression to intracellular H2O2 capacity of AM in TB patients. Design: In a prospective study, AM retrieved from 18 active pulmonary TB patients and 18 normal subjects were assessed for β2-integrin expression and intracellular H2O2 metabolism capacity by loading with anti-CD11a/CD18, anti-CD11b/CD18 monoclonal antibodies and 2',7' dichlorofluorescein diacetate (DCFH-DA) respectively, and analyzed by flow cytometry. AM from 8 normal subjects were stimulated with tumor necrosis factor-alpha (TNF-α, 105 units/ml) to examine the relationship between H2O2 production and CD11b/CD18 expression. Results: The magnitude of DCFH oxidation and CD11b/CD18 expression of AM was higher in TB patients than in normal subjects. The CD11b/CD18 expression was related to the magnitude of DCFH oxidation, but not to lymphocyte numbers or subpopulations (CD4, CD8, CD25). Stimulation of AM with TNF-α increased H2O2 production and CD11b/CD18 expression. Pretreatment with CD11b/CD18 monoclonal antibodies inhibited TNF-α-induced H2O2. Conclusion: AM in TB patients possessed a higher capacity of oxidant metabolism. The increased CD11b/CD18 expression may be related to the increased respiratory burst response in AM against mycobacterial invasion.

Original languageEnglish
Pages (from-to)468-475
Number of pages8
JournalTubercle and Lung Disease
Volume77
Issue number5
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

Alveolar Macrophages
Pulmonary Tuberculosis
Hydrogen Peroxide
Integrins
Tuberculosis
Monoclonal Antibodies
Respiratory Burst
Lymphocyte Subsets
Antigen Presentation
Lymphocyte Count
HLA Antigens
Mycobacterium tuberculosis
Phagocytosis
Oxidants
Reactive Oxygen Species
Flow Cytometry
Tumor Necrosis Factor-alpha
Prospective Studies

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Increased production off hydrogen peroxide and expression of CD11b/CD18 on alveolar macrophages in patients with active pulmonary tuberculosis. / Kuo, H. P.; Ho, T. C.; Wang, C. H.; Yu, C. T.; Lin, H. C.

In: Tubercle and Lung Disease, Vol. 77, No. 5, 01.01.1996, p. 468-475.

Research output: Contribution to journalArticle

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abstract = "Setting: Alveolar macrophages (AM) are important in host defense against Mycobacterium tuberculosis (TB). β2-integrins, especially CD11a/CD18 and CD11b/CD18, are implicated in leukocyte migration, antigen presentation, phagocytosis, and production of reactive oxygen species Objective: To explore the functional relevance of β2-integrin expression to intracellular H2O2 capacity of AM in TB patients. Design: In a prospective study, AM retrieved from 18 active pulmonary TB patients and 18 normal subjects were assessed for β2-integrin expression and intracellular H2O2 metabolism capacity by loading with anti-CD11a/CD18, anti-CD11b/CD18 monoclonal antibodies and 2',7' dichlorofluorescein diacetate (DCFH-DA) respectively, and analyzed by flow cytometry. AM from 8 normal subjects were stimulated with tumor necrosis factor-alpha (TNF-α, 105 units/ml) to examine the relationship between H2O2 production and CD11b/CD18 expression. Results: The magnitude of DCFH oxidation and CD11b/CD18 expression of AM was higher in TB patients than in normal subjects. The CD11b/CD18 expression was related to the magnitude of DCFH oxidation, but not to lymphocyte numbers or subpopulations (CD4, CD8, CD25). Stimulation of AM with TNF-α increased H2O2 production and CD11b/CD18 expression. Pretreatment with CD11b/CD18 monoclonal antibodies inhibited TNF-α-induced H2O2. Conclusion: AM in TB patients possessed a higher capacity of oxidant metabolism. The increased CD11b/CD18 expression may be related to the increased respiratory burst response in AM against mycobacterial invasion.",
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N2 - Setting: Alveolar macrophages (AM) are important in host defense against Mycobacterium tuberculosis (TB). β2-integrins, especially CD11a/CD18 and CD11b/CD18, are implicated in leukocyte migration, antigen presentation, phagocytosis, and production of reactive oxygen species Objective: To explore the functional relevance of β2-integrin expression to intracellular H2O2 capacity of AM in TB patients. Design: In a prospective study, AM retrieved from 18 active pulmonary TB patients and 18 normal subjects were assessed for β2-integrin expression and intracellular H2O2 metabolism capacity by loading with anti-CD11a/CD18, anti-CD11b/CD18 monoclonal antibodies and 2',7' dichlorofluorescein diacetate (DCFH-DA) respectively, and analyzed by flow cytometry. AM from 8 normal subjects were stimulated with tumor necrosis factor-alpha (TNF-α, 105 units/ml) to examine the relationship between H2O2 production and CD11b/CD18 expression. Results: The magnitude of DCFH oxidation and CD11b/CD18 expression of AM was higher in TB patients than in normal subjects. The CD11b/CD18 expression was related to the magnitude of DCFH oxidation, but not to lymphocyte numbers or subpopulations (CD4, CD8, CD25). Stimulation of AM with TNF-α increased H2O2 production and CD11b/CD18 expression. Pretreatment with CD11b/CD18 monoclonal antibodies inhibited TNF-α-induced H2O2. Conclusion: AM in TB patients possessed a higher capacity of oxidant metabolism. The increased CD11b/CD18 expression may be related to the increased respiratory burst response in AM against mycobacterial invasion.

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