Increased numbers of foxp3-positive regulatory T cells in gastritis, peptic ulcer and gastric adenocarcinoma

Hsin Hung Cheng, Guan Ying Tseng, Hsiao Bai Yang, Hung Jung Wang, Hwai Jeng Lin, Wen Ching Wang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

AIM: To determine the number of regulatory T cells (Tregs) in gastric mucosa of patients with gastritis, peptic ulcers and gastric cancer. METHODS: This study was a retrospective analysis of gastric antrum biopsy specimens from healthy controls (n = 22) and patients with gastritis (n = 30), peptic ulcer (n = 83), or gastric cancer (n = 32). Expression of CD4, CD25 and Foxp3 was determined by immunohis-tochemistry in three consecutive sections per sample. RESULTS: Compared with healthy controls, there was an increased number of CD25 + and Foxp3 + cells in patients with gastritis (P = 0.004 and P = 0.008), peptic ulcer (P <0.001 and P <0.001), and gastric cancer (P <0.001 and P <0.001). The ratio of CD25 +/CD4 + or Foxp3 +/CD4 + cells was also significantly higher in all disease groups (P <0.001, respectively). The number of CD4 +, CD25 +, and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were associated with the histological grade of the specimens, including acute inflammation, chronic inflammation, lymphoid follicle number, and Helicobacter pylori infection. The number of CD4 +, CD25 + and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were negatively associated with intestinal metaplasia among gastritis (P <0.001, P <0.001, P <0.001, P = 0.002 and P = 0.002) and peptic ulcer groups (P = 0.013, P = 0.004, P <0.001, P = 0.040 and P = 0.003). CONCLUSION: Tregs are positively associated with endoscopic findings of gastroduodenal diseases and histological grade but negatively associated with intestinal metaplasia in gastritis and peptic ulcer groups.

Original languageEnglish
Pages (from-to)34-43
Number of pages10
JournalWorld Journal of Gastroenterology
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 7 2012

Fingerprint

Gastritis
Regulatory T-Lymphocytes
Peptic Ulcer
Stomach
Adenocarcinoma
Stomach Neoplasms
Metaplasia
Inflammation
Pyloric Antrum
Helicobacter Infections
Gastric Mucosa
Helicobacter pylori
Biopsy

Keywords

  • Gas-troduodenal diseases
  • Helicobacter pylori
  • Immuno-histochemistry
  • Intestinal metaplasia
  • T regulatory cells

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Increased numbers of foxp3-positive regulatory T cells in gastritis, peptic ulcer and gastric adenocarcinoma. / Cheng, Hsin Hung; Tseng, Guan Ying; Yang, Hsiao Bai; Wang, Hung Jung; Lin, Hwai Jeng; Wang, Wen Ching.

In: World Journal of Gastroenterology, Vol. 18, No. 1, 07.01.2012, p. 34-43.

Research output: Contribution to journalArticle

Cheng, Hsin Hung ; Tseng, Guan Ying ; Yang, Hsiao Bai ; Wang, Hung Jung ; Lin, Hwai Jeng ; Wang, Wen Ching. / Increased numbers of foxp3-positive regulatory T cells in gastritis, peptic ulcer and gastric adenocarcinoma. In: World Journal of Gastroenterology. 2012 ; Vol. 18, No. 1. pp. 34-43.
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abstract = "AIM: To determine the number of regulatory T cells (Tregs) in gastric mucosa of patients with gastritis, peptic ulcers and gastric cancer. METHODS: This study was a retrospective analysis of gastric antrum biopsy specimens from healthy controls (n = 22) and patients with gastritis (n = 30), peptic ulcer (n = 83), or gastric cancer (n = 32). Expression of CD4, CD25 and Foxp3 was determined by immunohis-tochemistry in three consecutive sections per sample. RESULTS: Compared with healthy controls, there was an increased number of CD25 + and Foxp3 + cells in patients with gastritis (P = 0.004 and P = 0.008), peptic ulcer (P <0.001 and P <0.001), and gastric cancer (P <0.001 and P <0.001). The ratio of CD25 +/CD4 + or Foxp3 +/CD4 + cells was also significantly higher in all disease groups (P <0.001, respectively). The number of CD4 +, CD25 +, and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were associated with the histological grade of the specimens, including acute inflammation, chronic inflammation, lymphoid follicle number, and Helicobacter pylori infection. The number of CD4 +, CD25 + and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were negatively associated with intestinal metaplasia among gastritis (P <0.001, P <0.001, P <0.001, P = 0.002 and P = 0.002) and peptic ulcer groups (P = 0.013, P = 0.004, P <0.001, P = 0.040 and P = 0.003). CONCLUSION: Tregs are positively associated with endoscopic findings of gastroduodenal diseases and histological grade but negatively associated with intestinal metaplasia in gastritis and peptic ulcer groups.",
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T1 - Increased numbers of foxp3-positive regulatory T cells in gastritis, peptic ulcer and gastric adenocarcinoma

AU - Cheng, Hsin Hung

AU - Tseng, Guan Ying

AU - Yang, Hsiao Bai

AU - Wang, Hung Jung

AU - Lin, Hwai Jeng

AU - Wang, Wen Ching

PY - 2012/1/7

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N2 - AIM: To determine the number of regulatory T cells (Tregs) in gastric mucosa of patients with gastritis, peptic ulcers and gastric cancer. METHODS: This study was a retrospective analysis of gastric antrum biopsy specimens from healthy controls (n = 22) and patients with gastritis (n = 30), peptic ulcer (n = 83), or gastric cancer (n = 32). Expression of CD4, CD25 and Foxp3 was determined by immunohis-tochemistry in three consecutive sections per sample. RESULTS: Compared with healthy controls, there was an increased number of CD25 + and Foxp3 + cells in patients with gastritis (P = 0.004 and P = 0.008), peptic ulcer (P <0.001 and P <0.001), and gastric cancer (P <0.001 and P <0.001). The ratio of CD25 +/CD4 + or Foxp3 +/CD4 + cells was also significantly higher in all disease groups (P <0.001, respectively). The number of CD4 +, CD25 +, and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were associated with the histological grade of the specimens, including acute inflammation, chronic inflammation, lymphoid follicle number, and Helicobacter pylori infection. The number of CD4 +, CD25 + and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were negatively associated with intestinal metaplasia among gastritis (P <0.001, P <0.001, P <0.001, P = 0.002 and P = 0.002) and peptic ulcer groups (P = 0.013, P = 0.004, P <0.001, P = 0.040 and P = 0.003). CONCLUSION: Tregs are positively associated with endoscopic findings of gastroduodenal diseases and histological grade but negatively associated with intestinal metaplasia in gastritis and peptic ulcer groups.

AB - AIM: To determine the number of regulatory T cells (Tregs) in gastric mucosa of patients with gastritis, peptic ulcers and gastric cancer. METHODS: This study was a retrospective analysis of gastric antrum biopsy specimens from healthy controls (n = 22) and patients with gastritis (n = 30), peptic ulcer (n = 83), or gastric cancer (n = 32). Expression of CD4, CD25 and Foxp3 was determined by immunohis-tochemistry in three consecutive sections per sample. RESULTS: Compared with healthy controls, there was an increased number of CD25 + and Foxp3 + cells in patients with gastritis (P = 0.004 and P = 0.008), peptic ulcer (P <0.001 and P <0.001), and gastric cancer (P <0.001 and P <0.001). The ratio of CD25 +/CD4 + or Foxp3 +/CD4 + cells was also significantly higher in all disease groups (P <0.001, respectively). The number of CD4 +, CD25 +, and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were associated with the histological grade of the specimens, including acute inflammation, chronic inflammation, lymphoid follicle number, and Helicobacter pylori infection. The number of CD4 +, CD25 + and Foxp3 + cells, and the ratio of CD25 +/CD4 + and Foxp3 +/CD4 + cells, were negatively associated with intestinal metaplasia among gastritis (P <0.001, P <0.001, P <0.001, P = 0.002 and P = 0.002) and peptic ulcer groups (P = 0.013, P = 0.004, P <0.001, P = 0.040 and P = 0.003). CONCLUSION: Tregs are positively associated with endoscopic findings of gastroduodenal diseases and histological grade but negatively associated with intestinal metaplasia in gastritis and peptic ulcer groups.

KW - Gas-troduodenal diseases

KW - Helicobacter pylori

KW - Immuno-histochemistry

KW - Intestinal metaplasia

KW - T regulatory cells

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