Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in adenomyosis

Jehn Hsiahn Yang, Ming Yih Wu, Mei Jou Chen, Shee Uan Chen, Yu Shih Yang, Hong Nerng Ho

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To investigate cell invasiveness and the production of matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 in endometrial stromal cells (ESCs) of adenomyosis. Design: Prospective study. Setting: Tertiary university hospital. Patient(s): Twenty-one women with adenomyosis (study group) and 25 women without adenomyosis (control group). Intervention(s): Endometrial stromal cells were purified from eutopic endometrium and were cultured in vitro. Main Outcome Measure(s): Matrigel invasion assay and measurement of MMP-2, MMP-9, TIMP-1, and TIMP-2 concentrations. Result(s): Endometrial stromal cell invasiveness of adenomyosis was not different from that of the control group (0.95 vs. 1, using the latter as the bench mark) in ESCs cultured alone. After the ESCs were cultured with IL-6, anti-IL-6, or GM6001 for 24 hours, the indices of cell invasion were not different between the two groups. MMP-2 (median 7.3 vs. 3.5 ng/mL) and TIMP-1 (median 12.1 vs. 3.9 ng/mL), but not MMP-9 and TIMP-2, secreted by ESCs in women with adenomyosis were much higher than those in women without adenomyosis. Conclusion(s): The formation of adenomyosis does not result from altered invasiveness of ESCs. The concomitant elevation of MMP-2 and TIMP-1 may partially justify why invasiveness was not increased, but other enzymes should also be considered.

Original languageEnglish
Pages (from-to)2193-2198
Number of pages6
JournalFertility and Sterility
Volume91
Issue number5 SUPPL.
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

Fingerprint

Adenomyosis
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 2
Stromal Cells
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinases
Interleukin-6
Control Groups
Endometrium
Tertiary Care Centers
Outcome Assessment (Health Care)
Prospective Studies
Enzymes

Keywords

  • Adenomyosis
  • cell invasion
  • endometrial stromal cell
  • MMP
  • TIMP

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in adenomyosis. / Yang, Jehn Hsiahn; Wu, Ming Yih; Chen, Mei Jou; Chen, Shee Uan; Yang, Yu Shih; Ho, Hong Nerng.

In: Fertility and Sterility, Vol. 91, No. 5 SUPPL., 01.05.2009, p. 2193-2198.

Research output: Contribution to journalArticle

Yang, Jehn Hsiahn ; Wu, Ming Yih ; Chen, Mei Jou ; Chen, Shee Uan ; Yang, Yu Shih ; Ho, Hong Nerng. / Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in adenomyosis. In: Fertility and Sterility. 2009 ; Vol. 91, No. 5 SUPPL. pp. 2193-2198.
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AU - Yang, Jehn Hsiahn

AU - Wu, Ming Yih

AU - Chen, Mei Jou

AU - Chen, Shee Uan

AU - Yang, Yu Shih

AU - Ho, Hong Nerng

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AB - Objective: To investigate cell invasiveness and the production of matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 in endometrial stromal cells (ESCs) of adenomyosis. Design: Prospective study. Setting: Tertiary university hospital. Patient(s): Twenty-one women with adenomyosis (study group) and 25 women without adenomyosis (control group). Intervention(s): Endometrial stromal cells were purified from eutopic endometrium and were cultured in vitro. Main Outcome Measure(s): Matrigel invasion assay and measurement of MMP-2, MMP-9, TIMP-1, and TIMP-2 concentrations. Result(s): Endometrial stromal cell invasiveness of adenomyosis was not different from that of the control group (0.95 vs. 1, using the latter as the bench mark) in ESCs cultured alone. After the ESCs were cultured with IL-6, anti-IL-6, or GM6001 for 24 hours, the indices of cell invasion were not different between the two groups. MMP-2 (median 7.3 vs. 3.5 ng/mL) and TIMP-1 (median 12.1 vs. 3.9 ng/mL), but not MMP-9 and TIMP-2, secreted by ESCs in women with adenomyosis were much higher than those in women without adenomyosis. Conclusion(s): The formation of adenomyosis does not result from altered invasiveness of ESCs. The concomitant elevation of MMP-2 and TIMP-1 may partially justify why invasiveness was not increased, but other enzymes should also be considered.

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KW - TIMP

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