Increased expressions of CD69 and HLA-DR but not of CD25 or CD71 on endometrial T lymphocytes of nonpregnant women

Chun Kai Chen, Su Cheng Huang, Chi Long Chen, Min Ru Yen, Hey Chi Hsu, Hong Nerng Ho

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

To investigate lymphocyte subpopulations and the status of T-cell activation at different phases of the menstrual cycle, lymphocytes in endometrial tissue were analyzed by dual-color flow cytometry in 39 patients. Compared with peripheral blood, the lymphocytes in the endometrium had a higher CD3- (CD56+or CD16+) ratio (25.2% ± 6.8% vs 11.1% ± 7.0%), but an inverted CD3+CD8- CD3+CD8+ ratio (0.5 vs 1.8) and a minimal amount of B cells (3.3% ± 3.1%). TcR γδ+ T cells accounted for a minor proportion (7.8% ± 5.1%) in endometrium. The proportions of TcR αβ+ (85.0% ± 6.6%) and CD3+CD56+ (7.4% ± 4.4%) endometrial T lymphocytes were found significantly different from those in peripheral blood (89.1% ± 5.6% and 3.8% ± 3.4%, respectively). As the endometrium proceeded from proliferative phase to luteal phase, the proportion of CD3+CD8+ T cells in peripheral blood increased from 35.6% ± 6.9% to 41.3% ± 8.4% and CD3+CD8- T cells decreased from 64.4% ± 6.9% to 58.7% ± 8.4%. The endometrial T cells expressed high levels of CD69 (84.1% ± 18.9%) and DR (75.9% ± 9.7%), but rarely expressed CD25 (7.0% ± 5.4%) and CD71 (2.8% ± 1.8%). The patterns of expression of these activation markers were similar in both proliferative and luteal phases. Our observations suggest that endometrial T lymphocytes are in a state of recent and persistent activation. Lymphocytes expressing the NK cell markers (CD56 or CD16) and CD8+ accounted for a significant proportion, suggesting that they may play important roles in local defense. Human Immunology 42, 227-232 (1995).

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalHuman Immunology
Volume42
Issue number3
DOIs
Publication statusPublished - 1995
Externally publishedYes

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HLA-DR Antigens
T-Lymphocytes
Endometrium
Luteal Phase
Lymphocytes
Lymphocyte Subsets
Menstrual Cycle
Allergy and Immunology
Natural Killer Cells
Flow Cytometry
B-Lymphocytes
Color

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Increased expressions of CD69 and HLA-DR but not of CD25 or CD71 on endometrial T lymphocytes of nonpregnant women. / Chen, Chun Kai; Huang, Su Cheng; Chen, Chi Long; Yen, Min Ru; Hsu, Hey Chi; Ho, Hong Nerng.

In: Human Immunology, Vol. 42, No. 3, 1995, p. 227-232.

Research output: Contribution to journalArticle

Chen, Chun Kai ; Huang, Su Cheng ; Chen, Chi Long ; Yen, Min Ru ; Hsu, Hey Chi ; Ho, Hong Nerng. / Increased expressions of CD69 and HLA-DR but not of CD25 or CD71 on endometrial T lymphocytes of nonpregnant women. In: Human Immunology. 1995 ; Vol. 42, No. 3. pp. 227-232.
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abstract = "To investigate lymphocyte subpopulations and the status of T-cell activation at different phases of the menstrual cycle, lymphocytes in endometrial tissue were analyzed by dual-color flow cytometry in 39 patients. Compared with peripheral blood, the lymphocytes in the endometrium had a higher CD3- (CD56+or CD16+) ratio (25.2{\%} ± 6.8{\%} vs 11.1{\%} ± 7.0{\%}), but an inverted CD3+CD8- CD3+CD8+ ratio (0.5 vs 1.8) and a minimal amount of B cells (3.3{\%} ± 3.1{\%}). TcR γδ+ T cells accounted for a minor proportion (7.8{\%} ± 5.1{\%}) in endometrium. The proportions of TcR αβ+ (85.0{\%} ± 6.6{\%}) and CD3+CD56+ (7.4{\%} ± 4.4{\%}) endometrial T lymphocytes were found significantly different from those in peripheral blood (89.1{\%} ± 5.6{\%} and 3.8{\%} ± 3.4{\%}, respectively). As the endometrium proceeded from proliferative phase to luteal phase, the proportion of CD3+CD8+ T cells in peripheral blood increased from 35.6{\%} ± 6.9{\%} to 41.3{\%} ± 8.4{\%} and CD3+CD8- T cells decreased from 64.4{\%} ± 6.9{\%} to 58.7{\%} ± 8.4{\%}. The endometrial T cells expressed high levels of CD69 (84.1{\%} ± 18.9{\%}) and DR (75.9{\%} ± 9.7{\%}), but rarely expressed CD25 (7.0{\%} ± 5.4{\%}) and CD71 (2.8{\%} ± 1.8{\%}). The patterns of expression of these activation markers were similar in both proliferative and luteal phases. Our observations suggest that endometrial T lymphocytes are in a state of recent and persistent activation. Lymphocytes expressing the NK cell markers (CD56 or CD16) and CD8+ accounted for a significant proportion, suggesting that they may play important roles in local defense. Human Immunology 42, 227-232 (1995).",
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AU - Hsu, Hey Chi

AU - Ho, Hong Nerng

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N2 - To investigate lymphocyte subpopulations and the status of T-cell activation at different phases of the menstrual cycle, lymphocytes in endometrial tissue were analyzed by dual-color flow cytometry in 39 patients. Compared with peripheral blood, the lymphocytes in the endometrium had a higher CD3- (CD56+or CD16+) ratio (25.2% ± 6.8% vs 11.1% ± 7.0%), but an inverted CD3+CD8- CD3+CD8+ ratio (0.5 vs 1.8) and a minimal amount of B cells (3.3% ± 3.1%). TcR γδ+ T cells accounted for a minor proportion (7.8% ± 5.1%) in endometrium. The proportions of TcR αβ+ (85.0% ± 6.6%) and CD3+CD56+ (7.4% ± 4.4%) endometrial T lymphocytes were found significantly different from those in peripheral blood (89.1% ± 5.6% and 3.8% ± 3.4%, respectively). As the endometrium proceeded from proliferative phase to luteal phase, the proportion of CD3+CD8+ T cells in peripheral blood increased from 35.6% ± 6.9% to 41.3% ± 8.4% and CD3+CD8- T cells decreased from 64.4% ± 6.9% to 58.7% ± 8.4%. The endometrial T cells expressed high levels of CD69 (84.1% ± 18.9%) and DR (75.9% ± 9.7%), but rarely expressed CD25 (7.0% ± 5.4%) and CD71 (2.8% ± 1.8%). The patterns of expression of these activation markers were similar in both proliferative and luteal phases. Our observations suggest that endometrial T lymphocytes are in a state of recent and persistent activation. Lymphocytes expressing the NK cell markers (CD56 or CD16) and CD8+ accounted for a significant proportion, suggesting that they may play important roles in local defense. Human Immunology 42, 227-232 (1995).

AB - To investigate lymphocyte subpopulations and the status of T-cell activation at different phases of the menstrual cycle, lymphocytes in endometrial tissue were analyzed by dual-color flow cytometry in 39 patients. Compared with peripheral blood, the lymphocytes in the endometrium had a higher CD3- (CD56+or CD16+) ratio (25.2% ± 6.8% vs 11.1% ± 7.0%), but an inverted CD3+CD8- CD3+CD8+ ratio (0.5 vs 1.8) and a minimal amount of B cells (3.3% ± 3.1%). TcR γδ+ T cells accounted for a minor proportion (7.8% ± 5.1%) in endometrium. The proportions of TcR αβ+ (85.0% ± 6.6%) and CD3+CD56+ (7.4% ± 4.4%) endometrial T lymphocytes were found significantly different from those in peripheral blood (89.1% ± 5.6% and 3.8% ± 3.4%, respectively). As the endometrium proceeded from proliferative phase to luteal phase, the proportion of CD3+CD8+ T cells in peripheral blood increased from 35.6% ± 6.9% to 41.3% ± 8.4% and CD3+CD8- T cells decreased from 64.4% ± 6.9% to 58.7% ± 8.4%. The endometrial T cells expressed high levels of CD69 (84.1% ± 18.9%) and DR (75.9% ± 9.7%), but rarely expressed CD25 (7.0% ± 5.4%) and CD71 (2.8% ± 1.8%). The patterns of expression of these activation markers were similar in both proliferative and luteal phases. Our observations suggest that endometrial T lymphocytes are in a state of recent and persistent activation. Lymphocytes expressing the NK cell markers (CD56 or CD16) and CD8+ accounted for a significant proportion, suggesting that they may play important roles in local defense. Human Immunology 42, 227-232 (1995).

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