Increased expression of extracellular matrix proteins in rapid atrial pacing-induced atrial fibrillation

Chih Sheng Lin, Ling Ping Lai, Jiunn Lee Lin, Yu Ling Sun, Chih Wei Hsu, Chien Lung Chen, Simon J.T. Mao, Shoei K.Stephen Huang

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background: Atrial fibrillation (AF) is characterized by structural remodeling of the extracellular matrix (ECM) in cardiac atrium. Objective: The purpose of this study was to gain further insight into atrial ECM remodeling at the molecular level and to test whether altered expression of ECM proteins was associated with the disease. Methods: Sustained AF was induced in nine adult pigs after 3-4 weeks of continuous rapid atrial pacing at a rate of 600 bpm. Histologic studies and immunohistochemical stain were performed to identify the potential pathologic substrate underlying abnormalities in atrial tissues with sustained AF. Results: In the pathologic findings, the fraction of myocardial ECM (ECM%) was measured, with a significantly greater ECM% found in the AF group compared with the sham operated group (n = 6; i.e., pigs with normal sinus rhythm [SR]). A set of 9,182 genes was screened with cDNA microarray analysis. In AF animals, expression of 121 genes increased and 24 genes decreased by ≥1.75-fold compared with SR animals. Significant up-regulation of fibronectin-1 (4.9-fold), fibrillin-1 (3.1-fold), and fibromodulin (1.9-fold) in the fibrillating atria was confirmed by quantitative real-time reverse transcriptase-polymerase chain reaction. Western blot analysis revealed significantly increased atrial fibronectin-1, fibrillin-1, and fibromodulin in the AF group compared with the SR group (1.5-, 2.7-, and 2.1-fold, respectively). Immunohistochemical staining of AF tissue displayed increased accumulation of fibronectin-1 and fibrillin-1 in the atrial interstitial space. Conclusion: Increased expression of ECM proteins in fibrillating atria supports the hypothesis that ECM metabolism contributes to the development of AF.

Original languageEnglish
Pages (from-to)938-949
Number of pages12
JournalHeart Rhythm
Volume4
Issue number7
DOIs
Publication statusPublished - Jul 1 2007
Externally publishedYes

Fingerprint

Extracellular Matrix Proteins
Atrial Fibrillation
Extracellular Matrix
Fibronectins
Swine
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Genes
Real-Time Polymerase Chain Reaction
Coloring Agents
Up-Regulation
Western Blotting
Staining and Labeling
Gene Expression

Keywords

  • Atrial fibrillation
  • cDNA microarray
  • Extracellular matrix
  • Fibrillin
  • Fibronectin
  • Pig

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Increased expression of extracellular matrix proteins in rapid atrial pacing-induced atrial fibrillation. / Lin, Chih Sheng; Lai, Ling Ping; Lin, Jiunn Lee; Sun, Yu Ling; Hsu, Chih Wei; Chen, Chien Lung; Mao, Simon J.T.; Huang, Shoei K.Stephen.

In: Heart Rhythm, Vol. 4, No. 7, 01.07.2007, p. 938-949.

Research output: Contribution to journalArticle

Lin, Chih Sheng ; Lai, Ling Ping ; Lin, Jiunn Lee ; Sun, Yu Ling ; Hsu, Chih Wei ; Chen, Chien Lung ; Mao, Simon J.T. ; Huang, Shoei K.Stephen. / Increased expression of extracellular matrix proteins in rapid atrial pacing-induced atrial fibrillation. In: Heart Rhythm. 2007 ; Vol. 4, No. 7. pp. 938-949.
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abstract = "Background: Atrial fibrillation (AF) is characterized by structural remodeling of the extracellular matrix (ECM) in cardiac atrium. Objective: The purpose of this study was to gain further insight into atrial ECM remodeling at the molecular level and to test whether altered expression of ECM proteins was associated with the disease. Methods: Sustained AF was induced in nine adult pigs after 3-4 weeks of continuous rapid atrial pacing at a rate of 600 bpm. Histologic studies and immunohistochemical stain were performed to identify the potential pathologic substrate underlying abnormalities in atrial tissues with sustained AF. Results: In the pathologic findings, the fraction of myocardial ECM (ECM{\%}) was measured, with a significantly greater ECM{\%} found in the AF group compared with the sham operated group (n = 6; i.e., pigs with normal sinus rhythm [SR]). A set of 9,182 genes was screened with cDNA microarray analysis. In AF animals, expression of 121 genes increased and 24 genes decreased by ≥1.75-fold compared with SR animals. Significant up-regulation of fibronectin-1 (4.9-fold), fibrillin-1 (3.1-fold), and fibromodulin (1.9-fold) in the fibrillating atria was confirmed by quantitative real-time reverse transcriptase-polymerase chain reaction. Western blot analysis revealed significantly increased atrial fibronectin-1, fibrillin-1, and fibromodulin in the AF group compared with the SR group (1.5-, 2.7-, and 2.1-fold, respectively). Immunohistochemical staining of AF tissue displayed increased accumulation of fibronectin-1 and fibrillin-1 in the atrial interstitial space. Conclusion: Increased expression of ECM proteins in fibrillating atria supports the hypothesis that ECM metabolism contributes to the development of AF.",
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AU - Lin, Chih Sheng

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AU - Lin, Jiunn Lee

AU - Sun, Yu Ling

AU - Hsu, Chih Wei

AU - Chen, Chien Lung

AU - Mao, Simon J.T.

AU - Huang, Shoei K.Stephen

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AB - Background: Atrial fibrillation (AF) is characterized by structural remodeling of the extracellular matrix (ECM) in cardiac atrium. Objective: The purpose of this study was to gain further insight into atrial ECM remodeling at the molecular level and to test whether altered expression of ECM proteins was associated with the disease. Methods: Sustained AF was induced in nine adult pigs after 3-4 weeks of continuous rapid atrial pacing at a rate of 600 bpm. Histologic studies and immunohistochemical stain were performed to identify the potential pathologic substrate underlying abnormalities in atrial tissues with sustained AF. Results: In the pathologic findings, the fraction of myocardial ECM (ECM%) was measured, with a significantly greater ECM% found in the AF group compared with the sham operated group (n = 6; i.e., pigs with normal sinus rhythm [SR]). A set of 9,182 genes was screened with cDNA microarray analysis. In AF animals, expression of 121 genes increased and 24 genes decreased by ≥1.75-fold compared with SR animals. Significant up-regulation of fibronectin-1 (4.9-fold), fibrillin-1 (3.1-fold), and fibromodulin (1.9-fold) in the fibrillating atria was confirmed by quantitative real-time reverse transcriptase-polymerase chain reaction. Western blot analysis revealed significantly increased atrial fibronectin-1, fibrillin-1, and fibromodulin in the AF group compared with the SR group (1.5-, 2.7-, and 2.1-fold, respectively). Immunohistochemical staining of AF tissue displayed increased accumulation of fibronectin-1 and fibrillin-1 in the atrial interstitial space. Conclusion: Increased expression of ECM proteins in fibrillating atria supports the hypothesis that ECM metabolism contributes to the development of AF.

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