Increased circulating fibrocytes in asthma with chronic airflow obstruction

Chun Hua Wang, Chien Da Huang, Horng Chyuan Lin, Kang Yun Lee, Shu Min Lin, Chien Ying Liu, Kuo Hsiung Huang, Yu Shien Ko, Fan Chung Kian, Han Pin Kuo

Research output: Contribution to journalArticlepeer-review

148 Citations (Scopus)


Rationale: A proportion of patients with asthma present with chronic airflow obstruction (CAO). We hypothesized that this effect may result from increased activity of circulating fibroblast-like progenitor cells (fibrocytes) that could home to the airway mucosal wall. Objectives: To compare the proportion, proliferation, and differentiation of circulating fibrocytes from patients with asthma with CAO or no airflow obstruction (NOA) and control subjects. Methods: We investigated circulating fibrocytes in 11 patients with asthma with CAO and a rapid decline in FEV1, 9 patients with asthma with NOA, and 10 nonasthmatic control subjects. Blood nonadherent non-T (NANT) cells were incubated with fetal calf serum or each patient's own serum and fibrocytes expressing CD34, CD45, and collagen I with α-smooth muscle actin were identified by flow cytometry. Measurements and Main Results: A higher percentage of circulating fibrocytes in NANT cells was found in patients with CAO when compared with patients with NOA and control subjects. In CAO, the slope of the yearly decline in FEV1 correlated with circulating fibrocytes (r = -0.756, n = 11, P <0.01). When NANT cells from patients with CAO were cultured in the patients' own sera, more fibrocytes were detected than when cultured in sera from patients with NOA or from normal subjects. An anti-transforming growth factor (TGF)-β1-neutralizing antibody inhibited α-smooth muscle actin-positive fibrocyte transformation from NANT cells of patients with CAO. Serum TGF-β1 levels were higher in patients with CAO than in patients with NOA or in normal subjects. Conclusions: Circulating fibrocytes are increased in patients with asthma with CAO and can be transformed by TGF-β1 to myofibroblasts. Fibrocytes may contribute to airway obstruction in asthma.

Original languageEnglish
Pages (from-to)583-591
Number of pages9
JournalAmerican Journal of Respiratory and Critical Care Medicine
Issue number6
Publication statusPublished - Sept 15 2008
Externally publishedYes


  • Airway remodeling
  • Asthma
  • Fibrocytes
  • Myofibroblasts
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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