TY - JOUR
T1 - Increased Circulating Endothelial Apoptotic Microparticle to Endothelial Progenitor Cell Ratio Is Associated with Subsequent Decline in Glomerular Filtration Rate in Hypertensive Patients
AU - Hsu, Chien Yi
AU - Huang, Po Hsun
AU - Chiang, Chia Hung
AU - Leu, Hsin Bang
AU - Huang, Chin Chou
AU - Chen, Jaw Wen
AU - Lin, Shing Jong
PY - 2013/7/12
Y1 - 2013/7/12
N2 - Background:Recent research indicates hypertensive patients with microalbuminuria have decreased endothelial progenitor cells (EPCs) and increased levels of endothelial apoptotic microparticles (EMP). However, whether these changes are related to a subsequent decline in glomerular filtration rate (GFR) remains unclear.Methods and Results:We enrolled totally 100 hypertensive out-patients with eGFR ≥30 mL/min/1.73 m2. The mean annual rate of GFR decline (△GFR/y) was -1.49±3.26 mL/min/1.73 m2 per year during the follow-up period (34±6 months). Flow cytometry was used to assess circulating EPC (CD34+/KDR+) and EMP levels (CD31+/annexin V+) in peripheral blood. The △GFR/y was correlated with the EMP to EPC ratio (r = -0.465, p<0.001), microalbuminuria (r = -0.329, p = 0.001), and the Framingham risk score (r = -0.245, p = 0.013). When we divided the patients into 4 groups according to the EMP to EPC ratio, there was an association between the EMP to EPC ratio and the ΔGFR/y (mean ΔGFR/y: 0.08±3.04 vs. -0.50±2.84 vs. -1.25±2.49 vs. -4.42±2.82, p<0.001). Multivariate analysis indicated that increased EMP to EPC ratio is an independent predictor of ΔeGFR/y.Conclusions:An increased circulating EMP to EPC ratio is associated with subsequent decline in GFR in hypertensive patients, which suggests endothelial damage with reduced vascular repair capacity may contribute to further deterioration of renal function in patients with hypertension.
AB - Background:Recent research indicates hypertensive patients with microalbuminuria have decreased endothelial progenitor cells (EPCs) and increased levels of endothelial apoptotic microparticles (EMP). However, whether these changes are related to a subsequent decline in glomerular filtration rate (GFR) remains unclear.Methods and Results:We enrolled totally 100 hypertensive out-patients with eGFR ≥30 mL/min/1.73 m2. The mean annual rate of GFR decline (△GFR/y) was -1.49±3.26 mL/min/1.73 m2 per year during the follow-up period (34±6 months). Flow cytometry was used to assess circulating EPC (CD34+/KDR+) and EMP levels (CD31+/annexin V+) in peripheral blood. The △GFR/y was correlated with the EMP to EPC ratio (r = -0.465, p<0.001), microalbuminuria (r = -0.329, p = 0.001), and the Framingham risk score (r = -0.245, p = 0.013). When we divided the patients into 4 groups according to the EMP to EPC ratio, there was an association between the EMP to EPC ratio and the ΔGFR/y (mean ΔGFR/y: 0.08±3.04 vs. -0.50±2.84 vs. -1.25±2.49 vs. -4.42±2.82, p<0.001). Multivariate analysis indicated that increased EMP to EPC ratio is an independent predictor of ΔeGFR/y.Conclusions:An increased circulating EMP to EPC ratio is associated with subsequent decline in GFR in hypertensive patients, which suggests endothelial damage with reduced vascular repair capacity may contribute to further deterioration of renal function in patients with hypertension.
UR - http://www.scopus.com/inward/record.url?scp=84880067455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880067455&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0068644
DO - 10.1371/journal.pone.0068644
M3 - Article
C2 - 23874701
AN - SCOPUS:84880067455
SN - 1932-6203
VL - 8
JO - PLoS One
JF - PLoS One
IS - 7
M1 - e68644
ER -