Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells

Cheng Jeng Tai, Shing Chuan Shen, Woan Ruoh Lee, Ching Fong Liao, Win Ping Deng, Hung Yi Chiou, Cheng I. Hsieh, Jai Nien Tung, Ching Shyang Chen, Jeng Fong Chiou, Li Tzu Li, Chuang Yu Lin, Chung Huei Hsu, Ming Chung Jiang

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Abstract

Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.

Original languageEnglish
Pages (from-to)2969-2981
Number of pages13
JournalExperimental Cell Research
Volume316
Issue number17
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Cellular Apoptosis Susceptibility Protein
Tubulin
Apoptosis
Breast Neoplasms
Microtubules
Cell Movement
MCF-7 Cells
Neoplasms
Pseudopodia
Microtubule-Associated Proteins
Glutathione Transferase
Immunoprecipitation
Proteins
Fluorescence
Phosphorylation
Cell Membrane

Keywords

  • Cancer
  • Microtubules
  • Migration
  • Protrusion
  • Tubulin
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

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title = "Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells",
abstract = "Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.",
keywords = "Cancer, Microtubules, Migration, Protrusion, Tubulin, Tyrosine phosphorylation",
author = "Tai, {Cheng Jeng} and Shen, {Shing Chuan} and Lee, {Woan Ruoh} and Liao, {Ching Fong} and Deng, {Win Ping} and Chiou, {Hung Yi} and Hsieh, {Cheng I.} and Tung, {Jai Nien} and Chen, {Ching Shyang} and Chiou, {Jeng Fong} and Li, {Li Tzu} and Lin, {Chuang Yu} and Hsu, {Chung Huei} and Jiang, {Ming Chung}",
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T1 - Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells

AU - Tai, Cheng Jeng

AU - Shen, Shing Chuan

AU - Lee, Woan Ruoh

AU - Liao, Ching Fong

AU - Deng, Win Ping

AU - Chiou, Hung Yi

AU - Hsieh, Cheng I.

AU - Tung, Jai Nien

AU - Chen, Ching Shyang

AU - Chiou, Jeng Fong

AU - Li, Li Tzu

AU - Lin, Chuang Yu

AU - Hsu, Chung Huei

AU - Jiang, Ming Chung

PY - 2010/10

Y1 - 2010/10

N2 - Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.

AB - Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.

KW - Cancer

KW - Microtubules

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KW - Protrusion

KW - Tubulin

KW - Tyrosine phosphorylation

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