Increase of toll-like receptor 4 but decrease of interleukin-8 mRNA expression among ischemic stroke patients under aspirin treatment

Dean Wu, Jehn Shing Sheu, Hsing Cheng Liu, Rey Yue Yuan, Jia Ming Yu, Jau Jiuan Sheu, Chia Hsiu Hung, Chaur Jong Hu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: Toll-like receptors (TLRs) are molecules conserved in evolution for detecting pathogen invasions and tissue damage and are involved in atherogenesis. This study explores the mRNA expression of TLRs and their probable role in further disease occurrence among ischemic stroke patients. Design and Methods: A total of 89 ischemic stroke patients and 166 controls were recruited for this study. Total RNA was extracted and mRNA was reverse-transcribed to cDNA and was analyzed for TLRs and interleukin 8 (IL8). Results: The TLR4 mRNA expression level is significantly higher in the stroke group. Conversely, IL-8 mRNA levels decreased significantly in the patient group. Conclusion: Our results suggest that TLR4 overexpression in mRNA levels is observed in stroke patients, which might account for the probable inflammatory injury before or after stroke. A reduction of IL-8 expression could result from the downregulatory effects of aspirin.

Original languageEnglish
Pages (from-to)1316-1319
Number of pages4
JournalClinical Biochemistry
Volume45
Issue number16-17
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Toll-Like Receptor 4
Interleukin-8
Aspirin
Stroke
Messenger RNA
Toll-Like Receptors
Toll-Like Receptor 8
Therapeutics
Pathogens
Atherosclerosis
Complementary DNA
RNA
Tissue
Molecules
Wounds and Injuries

Keywords

  • Aspirin
  • Interleukin-8
  • Ischemic stroke
  • MRNA
  • Toll-like receptors

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Increase of toll-like receptor 4 but decrease of interleukin-8 mRNA expression among ischemic stroke patients under aspirin treatment. / Wu, Dean; Sheu, Jehn Shing; Liu, Hsing Cheng; Yuan, Rey Yue; Yu, Jia Ming; Sheu, Jau Jiuan; Hung, Chia Hsiu; Hu, Chaur Jong.

In: Clinical Biochemistry, Vol. 45, No. 16-17, 11.2012, p. 1316-1319.

Research output: Contribution to journalArticle

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AU - Sheu, Jehn Shing

AU - Liu, Hsing Cheng

AU - Yuan, Rey Yue

AU - Yu, Jia Ming

AU - Sheu, Jau Jiuan

AU - Hung, Chia Hsiu

AU - Hu, Chaur Jong

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N2 - Objectives: Toll-like receptors (TLRs) are molecules conserved in evolution for detecting pathogen invasions and tissue damage and are involved in atherogenesis. This study explores the mRNA expression of TLRs and their probable role in further disease occurrence among ischemic stroke patients. Design and Methods: A total of 89 ischemic stroke patients and 166 controls were recruited for this study. Total RNA was extracted and mRNA was reverse-transcribed to cDNA and was analyzed for TLRs and interleukin 8 (IL8). Results: The TLR4 mRNA expression level is significantly higher in the stroke group. Conversely, IL-8 mRNA levels decreased significantly in the patient group. Conclusion: Our results suggest that TLR4 overexpression in mRNA levels is observed in stroke patients, which might account for the probable inflammatory injury before or after stroke. A reduction of IL-8 expression could result from the downregulatory effects of aspirin.

AB - Objectives: Toll-like receptors (TLRs) are molecules conserved in evolution for detecting pathogen invasions and tissue damage and are involved in atherogenesis. This study explores the mRNA expression of TLRs and their probable role in further disease occurrence among ischemic stroke patients. Design and Methods: A total of 89 ischemic stroke patients and 166 controls were recruited for this study. Total RNA was extracted and mRNA was reverse-transcribed to cDNA and was analyzed for TLRs and interleukin 8 (IL8). Results: The TLR4 mRNA expression level is significantly higher in the stroke group. Conversely, IL-8 mRNA levels decreased significantly in the patient group. Conclusion: Our results suggest that TLR4 overexpression in mRNA levels is observed in stroke patients, which might account for the probable inflammatory injury before or after stroke. A reduction of IL-8 expression could result from the downregulatory effects of aspirin.

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KW - MRNA

KW - Toll-like receptors

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