Increase of oxidative stress in human sperm with lower motility

Shu Huei Kao, Hsiang Tai Chao, Haw Wen Chen, Yi-Sheng Huang, Tien Ling Liao, Yau Huei Wei

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Objective: To investigate the causal role of oxidative-stress status on human sperm motility. Design: To demonstrate that sperm with higher oxidative damage have a lower antioxidant capacity. Setting: University hospital infertility center. Patient(s): Seventy-eight semen samples were obtained from 35 healthy donors who had normal semen characteristics and from 43 infertile or subfertile males. Intervention(s): The levels of oxidative damage (8-hydroxy-2′-deoxyguanosine [8-OHdG] and lipid peroxides) and antioxidants (retinol, α-tocopherol, ascorbate, and protein thiols) in the spermatozoa and/or seminal plasma were measured. Main Outcome Measure(s): We analyzed the specific content of 8-OHdG and lipid peroxides by using high-performance liquid chromatography (HPLC)-electrochemical detection and HPLC-fluorescence analysis, respectively. Retinol and α-tocopherol were analyzed by using an HPLC system, whereas ascorbate and protein thiols were determined by using spectrophotometry. 8-Hydroxy-2′-deoxyguanosine was visualized by immunofluorescent staining with an anti-8-OHdG antibody that was conjugated with fluorescein isothiocyanate conjugate. Lipid peroxides in spermatozoa were stained with a fluorescent dye, C11-BODIPY581/591. Result(s): Statistically significant negative correlations were revealed between sperm motility and 8-OHdG and between motility and lipid peroxides. Statistically significant positive correlations were found between sperm motility and the levels of retinol, α-tocopherol, ascorbate, and protein thiols of seminal plasma. 8-Hydroxy-2′-deoxyguanosine and lipid peroxides in spermatozoa were found to be present mostly in mitochondria. Conclusion(s): Oxidative stress and oxidative damage were increased significantly in spermatozoa with declined motility, and the antioxidant capacities in the spermatozoa and seminal plasma were lower in males who had infertility or subfertility.

Original languageEnglish
Pages (from-to)1183-1190
Number of pages8
JournalFertility and Sterility
Volume89
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint

Lipid Peroxides
Spermatozoa
Oxidative Stress
Semen
Tocopherols
Sperm Motility
Vitamin A
Sulfhydryl Compounds
Infertility
Antioxidants
High Pressure Liquid Chromatography
Seminal Plasma Proteins
Spectrophotometry
Fluorescein
Fluorescent Dyes
Mitochondria
Proteins
Fluorescence
Outcome Assessment (Health Care)
Tissue Donors

Keywords

  • antioxidant capacity
  • motility
  • oxidative stress
  • Spermatozoa

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Kao, S. H., Chao, H. T., Chen, H. W., Huang, Y-S., Liao, T. L., & Wei, Y. H. (2008). Increase of oxidative stress in human sperm with lower motility. Fertility and Sterility, 89(5), 1183-1190. https://doi.org/10.1016/j.fertnstert.2007.05.029

Increase of oxidative stress in human sperm with lower motility. / Kao, Shu Huei; Chao, Hsiang Tai; Chen, Haw Wen; Huang, Yi-Sheng; Liao, Tien Ling; Wei, Yau Huei.

In: Fertility and Sterility, Vol. 89, No. 5, 05.2008, p. 1183-1190.

Research output: Contribution to journalArticle

Kao, SH, Chao, HT, Chen, HW, Huang, Y-S, Liao, TL & Wei, YH 2008, 'Increase of oxidative stress in human sperm with lower motility', Fertility and Sterility, vol. 89, no. 5, pp. 1183-1190. https://doi.org/10.1016/j.fertnstert.2007.05.029
Kao, Shu Huei ; Chao, Hsiang Tai ; Chen, Haw Wen ; Huang, Yi-Sheng ; Liao, Tien Ling ; Wei, Yau Huei. / Increase of oxidative stress in human sperm with lower motility. In: Fertility and Sterility. 2008 ; Vol. 89, No. 5. pp. 1183-1190.
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