Increase of adenosine A1 receptor gene expression in cerebral ischemia of Wistar rats

Dar Ming Lai, Yong Kwang Tu, I. M. Liu, Juei Tang Cheng

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In an attempt to know the role of adenosine A1 receptor in cerebral ischemia, the present study employed the ligation of bilateral carotid arteries to induce ischemia in Wistar rats. Changes of gene expression of adenosine A1 receptor in cerebral cortex of ischemic rats were compared with normal sham control and reperfusion group that received regular blood flow after a transient ischemia. The mRNA level of adenosine A1 receptor in cerebral cortex was markedly raised by this artificial ischemia. Also, reperfusion reversed this elevation to a level near the control. This change was also observed at the protein level using Western blot analysis of adenosine A1 receptor. The raised protein level of adenosine A1 receptor by ischemia was reversed to normal level after reperfusion. These data suggest that the gene expression of adenosine A1 receptor was increased by ischemia probably due to the compensative response of brain. The raised adenosine A1 receptor may play a protective role in these damaged tissues.

Original languageEnglish
Pages (from-to)59-61
Number of pages3
JournalNeuroscience Letters
Volume387
Issue number2
DOIs
Publication statusPublished - Oct 21 2005
Externally publishedYes

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Adenosine A1 Receptors
Brain Ischemia
Wistar Rats
Gene Expression
Ischemia
Reperfusion
Cerebral Cortex
Carotid Arteries
Ligation
Proteins
Western Blotting
Control Groups
Messenger RNA
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

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Increase of adenosine A1 receptor gene expression in cerebral ischemia of Wistar rats. / Lai, Dar Ming; Tu, Yong Kwang; Liu, I. M.; Cheng, Juei Tang.

In: Neuroscience Letters, Vol. 387, No. 2, 21.10.2005, p. 59-61.

Research output: Contribution to journalArticle

Lai, Dar Ming ; Tu, Yong Kwang ; Liu, I. M. ; Cheng, Juei Tang. / Increase of adenosine A1 receptor gene expression in cerebral ischemia of Wistar rats. In: Neuroscience Letters. 2005 ; Vol. 387, No. 2. pp. 59-61.
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