Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits

Y. H. Jean, Z. H. Wen, Y. C. Chang, S. P. Hsieh, J. D. Lin, C. C. Tang, W. F. Chen, A. K. Chou, C. S. Wong

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: The present study aimed to determine the role of excitatory amino acids (EAAs) and EAA transporters (EAATs) in an osteoarthritis (OA) model of rabbit knees. Methods: OA was induced in New Zealand white male rabbits by anterior cruciate ligament transection (ACLT) in one knee of one hind limb; the other knee left unoperated. Rabbits that received ACLT of knee were assigned to the ACLT group (n = 6), while a sham-operated group (n = 6) underwent arthrotomy with no ACLT. Six naïve rabbits that received no surgery were used as normal control. The width of the knee joint was measured to determine the severity of joint inflammation. Before operation and at 10, 20, and 30 weeks after operation, knee joint dialysates were collected by microdialysis and assayed for EAAs by high-performance liquid chromatography. Gross morphology and histopathology and EAATs glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) expression in the articular cartilage of the knees were evaluated by immunohistochemistry and western blot analysis. Results: In the ACLT knees, a significant increase in the joint width was observed (5.3 ± 0.9 mm, P < 0.05) at 30 weeks after operation, while the sham-operated and naïve knees showed no difference as compared with the basal values. The concentrations (μM) of aspartate and glutamate in knee dialysates at 30 weeks after ACLT in naïve, sham, and ACLT were 0.36 ± 0.07 and 4.5 ± 1.10; 0.38 ± 0.09 and 4.61 ± 1.11; 0.67 ± 0.18 and 9.71 ± 2.89, respectively. Levels of glutamate and aspartate in the dialysates obtained from the ACLT knees increased by 213.3 ± 29.6% and 187.5 ± 33.8% (P < 0.05) when compared to those in the sham-operated knees. Both naïve and ACLT chondrocytes were positively stained by antibodies against GLAST and GLT-1. GLAST and GLT-1 protein expressions were significantly increased in the ACLT knees (P < 0.05). Conclusion: Our findings indicate an involvement of EAAs and EAATs in the pathogenesis of OA in ACLT rabbits.

Original languageEnglish
Pages (from-to)1442-1449
Number of pages8
JournalOsteoarthritis and Cartilage
Volume16
Issue number12
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes

Fingerprint

Amino Acid Transport Systems
Excitatory Amino Acids
Anterior Cruciate Ligament
Ligaments
Amino acids
Knee
Amino Acid Transport System X-AG
Rabbits
Dialysis Solutions
Osteoarthritis
Knee Joint
Aspartic Acid
Glutamic Acid
Joints
Microdialysis
Cartilage
High performance liquid chromatography
Articular Cartilage
Chondrocytes
Antibodies

Keywords

  • Anterior cruciate ligament
  • Excitatory amino acid transporters
  • Glutamate
  • Glutamate transporters
  • Microdialysis
  • Osteoarthritis

ASJC Scopus subject areas

  • Rheumatology
  • Biomedical Engineering
  • Orthopedics and Sports Medicine

Cite this

Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits. / Jean, Y. H.; Wen, Z. H.; Chang, Y. C.; Hsieh, S. P.; Lin, J. D.; Tang, C. C.; Chen, W. F.; Chou, A. K.; Wong, C. S.

In: Osteoarthritis and Cartilage, Vol. 16, No. 12, 01.12.2008, p. 1442-1449.

Research output: Contribution to journalArticle

Jean, Y. H. ; Wen, Z. H. ; Chang, Y. C. ; Hsieh, S. P. ; Lin, J. D. ; Tang, C. C. ; Chen, W. F. ; Chou, A. K. ; Wong, C. S. / Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits. In: Osteoarthritis and Cartilage. 2008 ; Vol. 16, No. 12. pp. 1442-1449.
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abstract = "Objective: The present study aimed to determine the role of excitatory amino acids (EAAs) and EAA transporters (EAATs) in an osteoarthritis (OA) model of rabbit knees. Methods: OA was induced in New Zealand white male rabbits by anterior cruciate ligament transection (ACLT) in one knee of one hind limb; the other knee left unoperated. Rabbits that received ACLT of knee were assigned to the ACLT group (n = 6), while a sham-operated group (n = 6) underwent arthrotomy with no ACLT. Six na{\"i}ve rabbits that received no surgery were used as normal control. The width of the knee joint was measured to determine the severity of joint inflammation. Before operation and at 10, 20, and 30 weeks after operation, knee joint dialysates were collected by microdialysis and assayed for EAAs by high-performance liquid chromatography. Gross morphology and histopathology and EAATs glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) expression in the articular cartilage of the knees were evaluated by immunohistochemistry and western blot analysis. Results: In the ACLT knees, a significant increase in the joint width was observed (5.3 ± 0.9 mm, P < 0.05) at 30 weeks after operation, while the sham-operated and na{\"i}ve knees showed no difference as compared with the basal values. The concentrations (μM) of aspartate and glutamate in knee dialysates at 30 weeks after ACLT in na{\"i}ve, sham, and ACLT were 0.36 ± 0.07 and 4.5 ± 1.10; 0.38 ± 0.09 and 4.61 ± 1.11; 0.67 ± 0.18 and 9.71 ± 2.89, respectively. Levels of glutamate and aspartate in the dialysates obtained from the ACLT knees increased by 213.3 ± 29.6{\%} and 187.5 ± 33.8{\%} (P < 0.05) when compared to those in the sham-operated knees. Both na{\"i}ve and ACLT chondrocytes were positively stained by antibodies against GLAST and GLT-1. GLAST and GLT-1 protein expressions were significantly increased in the ACLT knees (P < 0.05). Conclusion: Our findings indicate an involvement of EAAs and EAATs in the pathogenesis of OA in ACLT rabbits.",
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T1 - Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits

AU - Jean, Y. H.

AU - Wen, Z. H.

AU - Chang, Y. C.

AU - Hsieh, S. P.

AU - Lin, J. D.

AU - Tang, C. C.

AU - Chen, W. F.

AU - Chou, A. K.

AU - Wong, C. S.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Objective: The present study aimed to determine the role of excitatory amino acids (EAAs) and EAA transporters (EAATs) in an osteoarthritis (OA) model of rabbit knees. Methods: OA was induced in New Zealand white male rabbits by anterior cruciate ligament transection (ACLT) in one knee of one hind limb; the other knee left unoperated. Rabbits that received ACLT of knee were assigned to the ACLT group (n = 6), while a sham-operated group (n = 6) underwent arthrotomy with no ACLT. Six naïve rabbits that received no surgery were used as normal control. The width of the knee joint was measured to determine the severity of joint inflammation. Before operation and at 10, 20, and 30 weeks after operation, knee joint dialysates were collected by microdialysis and assayed for EAAs by high-performance liquid chromatography. Gross morphology and histopathology and EAATs glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) expression in the articular cartilage of the knees were evaluated by immunohistochemistry and western blot analysis. Results: In the ACLT knees, a significant increase in the joint width was observed (5.3 ± 0.9 mm, P < 0.05) at 30 weeks after operation, while the sham-operated and naïve knees showed no difference as compared with the basal values. The concentrations (μM) of aspartate and glutamate in knee dialysates at 30 weeks after ACLT in naïve, sham, and ACLT were 0.36 ± 0.07 and 4.5 ± 1.10; 0.38 ± 0.09 and 4.61 ± 1.11; 0.67 ± 0.18 and 9.71 ± 2.89, respectively. Levels of glutamate and aspartate in the dialysates obtained from the ACLT knees increased by 213.3 ± 29.6% and 187.5 ± 33.8% (P < 0.05) when compared to those in the sham-operated knees. Both naïve and ACLT chondrocytes were positively stained by antibodies against GLAST and GLT-1. GLAST and GLT-1 protein expressions were significantly increased in the ACLT knees (P < 0.05). Conclusion: Our findings indicate an involvement of EAAs and EAATs in the pathogenesis of OA in ACLT rabbits.

AB - Objective: The present study aimed to determine the role of excitatory amino acids (EAAs) and EAA transporters (EAATs) in an osteoarthritis (OA) model of rabbit knees. Methods: OA was induced in New Zealand white male rabbits by anterior cruciate ligament transection (ACLT) in one knee of one hind limb; the other knee left unoperated. Rabbits that received ACLT of knee were assigned to the ACLT group (n = 6), while a sham-operated group (n = 6) underwent arthrotomy with no ACLT. Six naïve rabbits that received no surgery were used as normal control. The width of the knee joint was measured to determine the severity of joint inflammation. Before operation and at 10, 20, and 30 weeks after operation, knee joint dialysates were collected by microdialysis and assayed for EAAs by high-performance liquid chromatography. Gross morphology and histopathology and EAATs glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) expression in the articular cartilage of the knees were evaluated by immunohistochemistry and western blot analysis. Results: In the ACLT knees, a significant increase in the joint width was observed (5.3 ± 0.9 mm, P < 0.05) at 30 weeks after operation, while the sham-operated and naïve knees showed no difference as compared with the basal values. The concentrations (μM) of aspartate and glutamate in knee dialysates at 30 weeks after ACLT in naïve, sham, and ACLT were 0.36 ± 0.07 and 4.5 ± 1.10; 0.38 ± 0.09 and 4.61 ± 1.11; 0.67 ± 0.18 and 9.71 ± 2.89, respectively. Levels of glutamate and aspartate in the dialysates obtained from the ACLT knees increased by 213.3 ± 29.6% and 187.5 ± 33.8% (P < 0.05) when compared to those in the sham-operated knees. Both naïve and ACLT chondrocytes were positively stained by antibodies against GLAST and GLT-1. GLAST and GLT-1 protein expressions were significantly increased in the ACLT knees (P < 0.05). Conclusion: Our findings indicate an involvement of EAAs and EAATs in the pathogenesis of OA in ACLT rabbits.

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KW - Excitatory amino acid transporters

KW - Glutamate

KW - Glutamate transporters

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KW - Osteoarthritis

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