Incompatibility of Escherichia coli rho mutants with plasmids is mediated by plasmid-specific transcription

Tsai Kun Li, Yuri A. Panchenko, Marc Drolet, Leroy-Fong Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Escherichia coli rho-15 mutant (deficient in transcription termination) is known to be incompatible with pBR322 and other plasmids (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We show that failure of pBR322 to transform rho-15 is mediated by transcription from the tet promoter and readthrough from the tet gene into the rom region. Using an isopropyl-β-D-thiogalactopyranoside-inducible promoter to replace the tet promoter, we have demonstrated that plasmid- specific transcription inhibits growth of the rho-15 host, possibly due to the expression of the Rom protein. The involvement of Rom protein in pBR322- rho-15 incompatibility is further indicated by the following two experiments. (i) Functional inactivation of the rom gene in pBR322 enabled plasmids to transform E. coli rho-15. (ii) Specific overexpression of the rom gene abolished plasmid transformation into E. coli rho-15. An rpoB8(Ts) mutant RNA polymerase which compensated for the termination defect in E. coli rho-15 also restored plasmid-host compatibility, suggesting that Rom-mediated plasmid-host incompatibility is linked to a defect in transcription termination.

Original languageEnglish
Pages (from-to)5789-5794
Number of pages6
JournalJournal of Bacteriology
Volume179
Issue number18
Publication statusPublished - Sep 1997
Externally publishedYes

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Plasmids
Escherichia coli
Thiogalactosides
Viverridae
Gene Silencing
DNA-Directed RNA Polymerases
Genes
Proteins
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Incompatibility of Escherichia coli rho mutants with plasmids is mediated by plasmid-specific transcription. / Li, Tsai Kun; Panchenko, Yuri A.; Drolet, Marc; Liu, Leroy-Fong.

In: Journal of Bacteriology, Vol. 179, No. 18, 09.1997, p. 5789-5794.

Research output: Contribution to journalArticle

Li, Tsai Kun ; Panchenko, Yuri A. ; Drolet, Marc ; Liu, Leroy-Fong. / Incompatibility of Escherichia coli rho mutants with plasmids is mediated by plasmid-specific transcription. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 18. pp. 5789-5794.
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