Inactivation of lytic transglycosylases increases susceptibility to aminoglycosides and macrolides by altering the outer membrane permeability of Stenotrophomonas maltophilia

Chao Jung Wu, Yi Wei Huang, Yi Tsung Lin, Tsuey Ching Yang

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Stenotrophomonas maltophilia harbors six lytic transglycosylases (LTs): mltA, mltB1, mltB2, mltD1, mltD2, and slt. LT deletion increased susceptibility of S. maltophilia to aminoglycosides (AGs) and macrolides, and the underlying mechanisms were investigated. The expression of AG-modifying enzymes and efflux pumps was evaluated by quantitative reverse transcription-PCR (qRT-PCR). Susceptibility to 1-N-phenylnaphthylamine, vancomycin, SDS, and bile salts was measured to assess outer membrane permeability. In conclusion, increased outer membrane permeability contributes to LT deletion-mediated increase in aminoglycoside and macrolide susceptibility.

Original languageEnglish
Pages (from-to)3236-3239
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume60
Issue number5
DOIs
Publication statusPublished - May 2016
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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