Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeast

Haiyan Qi, Tsai Kun Li, Debbie Kuo, Alam Nur-E-Kamal, Leroy-Fong Liu

Research output: Contribution to journalArticle

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Abstract

Inactivation of the budding yeast telomere binding protein Cdc13 results in abnormal telomeres (exposed long G-strands) and activation of the DNA damage checkpoint. In the current study, we show that inactivation of Cdc13p induces apoptotic signals in yeast, as evidenced by caspase activation, increased reactive oxygen species production, and flipping of phosphatidylserine in the cytoplasmic membrane. These apoptotic signals were suppressed in a mitochondrial (ρ°) mutant. Moreover, mitochondrial proteins (e.g. MTCO3) were identified as multicopy suppressors of cdc13-1, suggesting the involvement of mitochondrial functions in telomere-initiated apoptotic signaling. These telomere-initiated apoptotic signals were also shown to depend on MEC1, but not TEL1, and were antagonized by MRE11. Our results are consistent with a model in which single-stranded G-tails in the cdc13-1 mutant trigger MEC1-dependent apoptotic signaling in yeast.

Original languageEnglish
Pages (from-to)15136-15141
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number17
DOIs
Publication statusPublished - Apr 25 2003
Externally publishedYes

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Telomere
Yeast
Yeasts
Telomere-Binding Proteins
Chemical activation
Saccharomycetales
Fungal Proteins
Mitochondrial Proteins
Phosphatidylserines
Caspases
DNA Damage
Reactive Oxygen Species
Cell Membrane
Membranes
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeast. / Qi, Haiyan; Li, Tsai Kun; Kuo, Debbie; Nur-E-Kamal, Alam; Liu, Leroy-Fong.

In: Journal of Biological Chemistry, Vol. 278, No. 17, 25.04.2003, p. 15136-15141.

Research output: Contribution to journalArticle

Qi, Haiyan ; Li, Tsai Kun ; Kuo, Debbie ; Nur-E-Kamal, Alam ; Liu, Leroy-Fong. / Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeast. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 17. pp. 15136-15141.
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