In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium

Yao Yuan Hsieh, Chich Sheng Lin, Yu Ling Sun, Chi Chen Chang, Horng Der Tsai, Jackson Chieh Hsi Wu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium. Materials and Methods: Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection. Results: LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414, 1.096, 1.162, and 0.782. In Group 2, LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32. Conclusions: Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Volume19
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Leukemia Inhibitory Factor
Endometrium
Genes
Luciferases
Liposomes
Superovulation
Mouse Lif protein
Inbred ICR Mouse
Glyceraldehyde-3-Phosphate Dehydrogenases
Reverse Transcription
Embryonic Development
Transfection
Plasmids
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Endometrium
  • Gene transfer
  • Leukemia inhibitory factor
  • LIF
  • Liposome

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics
  • Reproductive Medicine

Cite this

In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium. / Hsieh, Yao Yuan; Lin, Chich Sheng; Sun, Yu Ling; Chang, Chi Chen; Tsai, Horng Der; Wu, Jackson Chieh Hsi.

In: Journal of Assisted Reproduction and Genetics, Vol. 19, No. 2, 2002, p. 79-83.

Research output: Contribution to journalArticle

Hsieh, Yao Yuan ; Lin, Chich Sheng ; Sun, Yu Ling ; Chang, Chi Chen ; Tsai, Horng Der ; Wu, Jackson Chieh Hsi. / In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium. In: Journal of Assisted Reproduction and Genetics. 2002 ; Vol. 19, No. 2. pp. 79-83.
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