In vivo biocompatibility and degradability of a novel injectable-chitosan-based implant

Fwu Long Mi, Yu Chiun Tan, Hsiang F. Liang, Hsing W. Sung

Research output: Contribution to journalArticlepeer-review

500 Citations (Scopus)


A novel injectable-chitosan-based delivery system with low cytotoxicity was fabricated in the study. The chitosan microspheres with small particle size, low crystallinity and good sphericity were prepared by a spray-drying method followed by treating with a crosslinker. In the study, a naturally occurring crosslinking reagent (genipin), which has been used in herbal medicine and in the production of food dyes, was used to crosslink the chitosan microspheres. The glutaraldehyde-crosslinked counterparts were used as a control. Histological study of the genipin-crosslinked chitosan microspheres injected intramuscularly into the skeletal muscle of a rat model showed a less inflammatory reaction than its glutaraldehyde-crosslinked counterparts. The results of the scanning electron microscopic examination indicated that the glutaraldehyde-crosslinked chitosan microspheres retrieved at 12-week postoperatively were already degraded into a loose and porous structure. However, the degradation of the genipin-crosslinked chitosan microspheres was not significant after 20 weeks of implantation. The results of the study demonstrated that the genipin-crosslinked chitosan microspheres have a superior biocompatibility and a slower degradation rate than the glutaraldehyde-crosslinked chitosan microspheres. Accordingly, the genipin-crosslinked chitosan microspheres may be a suitable polymeric carrier for long-acting injectable drug delivery.

Original languageEnglish
Pages (from-to)181-191
Number of pages11
Issue number1
Publication statusPublished - Jan 1 2002
Externally publishedYes


  • Biocompatibility
  • Chitosan
  • Degradation
  • Genipin
  • Glutaraldehyde
  • Microspheres

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering


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