In vivo autofluorescence spectroscopy of oral premalignant and malignant lesions: Distortion of fluorescence intensity by submucous fibrosis

Tsuimin Tsai, Hsin Ming Chen, Chih Yu Wang, Jui Chang Tsai, Chin Tin Chen, Chun Pin Chiang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background and Objectives: To test whether autofluorescence spectroscopy can be used for the diagnosis of oral neoplasia in a high-risk population, we characterized the in vivo autofluorescence spectra from oral submucous fibrosis (OSF) lesions and oral premalignant and malignant lesions in both OSF and non-OSF patients. Study Design/Materials and Methods: Autofluorescence emission spectra were measured under the excitation wavelength of 330 nm, using a Xenon lamp-based fluorospectrometer coupled to a handheld optical fiber probe. Autofluorescence spectroscopies were analyzed among patients with OSF lesions, and oral lesions of epithelial hyperkeratosis (EH), epithelial dysplasia (ED), and squamous cell carcinomas (SCC) and normal oral mucosa (NOM) of healthy volunteers. Results: We found that the most intensely autofluorescence emission peaks occurred at 380 nm and 460 nm. For comparing the spectral patterns among different groups of oral lesions and NOM, ratios of the area under the spectrum of 460 ± 10 nm to that under the spectrum of 380 ± 10 nm (denoted as A460 ± 10 nm/A380 ± 10 nm) were calculated. The mean ratio values increased gradually from OSF to NOM, to EH and ED, and to SCC. The ANOVA test showed significant differences in the ratio value among all categories of samples (P <0.01). On the other hand, we found that EH, ED, and SCC lesions on OSF patients had distorted autofluorescence intensity. The mean ratio values of EH, ED, and SCC between non-OSF and OSF patients show significant differences. Furthermore, an ANOVA test showed NOM is not distinguishable from EH and ED lesions on oral fibrotic mucosa (P > 0.05). Conclusions: Autofluorescence spectroscopy can be used to diagnose EH, ED, and SCC lesions in non-OSF patients but not in OSF patients.

Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalLasers in Surgery and Medicine
Volume33
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

Oral Submucous Fibrosis
Spectrum Analysis
Fibrosis
Fluorescence
Mouth Mucosa
Squamous Cell Carcinoma
Optical Fibers
Oral Diagnosis
Xenon
Analysis of Variance
Healthy Volunteers
Population
Neoplasms

Keywords

  • Fluorescence spectroscopy
  • Oral neoplasia
  • Oral submucous fibrosis

ASJC Scopus subject areas

  • Surgery

Cite this

In vivo autofluorescence spectroscopy of oral premalignant and malignant lesions : Distortion of fluorescence intensity by submucous fibrosis. / Tsai, Tsuimin; Chen, Hsin Ming; Wang, Chih Yu; Tsai, Jui Chang; Chen, Chin Tin; Chiang, Chun Pin.

In: Lasers in Surgery and Medicine, Vol. 33, No. 1, 2003, p. 40-47.

Research output: Contribution to journalArticle

Tsai, Tsuimin ; Chen, Hsin Ming ; Wang, Chih Yu ; Tsai, Jui Chang ; Chen, Chin Tin ; Chiang, Chun Pin. / In vivo autofluorescence spectroscopy of oral premalignant and malignant lesions : Distortion of fluorescence intensity by submucous fibrosis. In: Lasers in Surgery and Medicine. 2003 ; Vol. 33, No. 1. pp. 40-47.
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abstract = "Background and Objectives: To test whether autofluorescence spectroscopy can be used for the diagnosis of oral neoplasia in a high-risk population, we characterized the in vivo autofluorescence spectra from oral submucous fibrosis (OSF) lesions and oral premalignant and malignant lesions in both OSF and non-OSF patients. Study Design/Materials and Methods: Autofluorescence emission spectra were measured under the excitation wavelength of 330 nm, using a Xenon lamp-based fluorospectrometer coupled to a handheld optical fiber probe. Autofluorescence spectroscopies were analyzed among patients with OSF lesions, and oral lesions of epithelial hyperkeratosis (EH), epithelial dysplasia (ED), and squamous cell carcinomas (SCC) and normal oral mucosa (NOM) of healthy volunteers. Results: We found that the most intensely autofluorescence emission peaks occurred at 380 nm and 460 nm. For comparing the spectral patterns among different groups of oral lesions and NOM, ratios of the area under the spectrum of 460 ± 10 nm to that under the spectrum of 380 ± 10 nm (denoted as A460 ± 10 nm/A380 ± 10 nm) were calculated. The mean ratio values increased gradually from OSF to NOM, to EH and ED, and to SCC. The ANOVA test showed significant differences in the ratio value among all categories of samples (P <0.01). On the other hand, we found that EH, ED, and SCC lesions on OSF patients had distorted autofluorescence intensity. The mean ratio values of EH, ED, and SCC between non-OSF and OSF patients show significant differences. Furthermore, an ANOVA test showed NOM is not distinguishable from EH and ED lesions on oral fibrotic mucosa (P > 0.05). Conclusions: Autofluorescence spectroscopy can be used to diagnose EH, ED, and SCC lesions in non-OSF patients but not in OSF patients.",
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T2 - Distortion of fluorescence intensity by submucous fibrosis

AU - Tsai, Tsuimin

AU - Chen, Hsin Ming

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AU - Tsai, Jui Chang

AU - Chen, Chin Tin

AU - Chiang, Chun Pin

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AB - Background and Objectives: To test whether autofluorescence spectroscopy can be used for the diagnosis of oral neoplasia in a high-risk population, we characterized the in vivo autofluorescence spectra from oral submucous fibrosis (OSF) lesions and oral premalignant and malignant lesions in both OSF and non-OSF patients. Study Design/Materials and Methods: Autofluorescence emission spectra were measured under the excitation wavelength of 330 nm, using a Xenon lamp-based fluorospectrometer coupled to a handheld optical fiber probe. Autofluorescence spectroscopies were analyzed among patients with OSF lesions, and oral lesions of epithelial hyperkeratosis (EH), epithelial dysplasia (ED), and squamous cell carcinomas (SCC) and normal oral mucosa (NOM) of healthy volunteers. Results: We found that the most intensely autofluorescence emission peaks occurred at 380 nm and 460 nm. For comparing the spectral patterns among different groups of oral lesions and NOM, ratios of the area under the spectrum of 460 ± 10 nm to that under the spectrum of 380 ± 10 nm (denoted as A460 ± 10 nm/A380 ± 10 nm) were calculated. The mean ratio values increased gradually from OSF to NOM, to EH and ED, and to SCC. The ANOVA test showed significant differences in the ratio value among all categories of samples (P <0.01). On the other hand, we found that EH, ED, and SCC lesions on OSF patients had distorted autofluorescence intensity. The mean ratio values of EH, ED, and SCC between non-OSF and OSF patients show significant differences. Furthermore, an ANOVA test showed NOM is not distinguishable from EH and ED lesions on oral fibrotic mucosa (P > 0.05). Conclusions: Autofluorescence spectroscopy can be used to diagnose EH, ED, and SCC lesions in non-OSF patients but not in OSF patients.

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KW - Oral neoplasia

KW - Oral submucous fibrosis

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