白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究

Translated title of the contribution: In Vitro Study of Crosslinked Albumin on Allylamine Plasma-Treated Titanium Surface

歐 耿良(Keng-Liang Ou), 林 耀楠(Yao-Nan Lin), 陳 正雄(Cheng-Hsiung Chen), 張 維仁(Wei-Jen Chang), 林 哲堂(Che-Tong Ling), 謝 松志(Sung-Chih Hsieh), 鄧 乃嘉(Nai-Chia Jeng), 李 勝揚(Sheng-Yang Lee)

Research output: Contribution to journalArticle

Abstract

In this study, our goal was to develop a glow-discharge method to functionalize titanium surfaces by the covalent immobilization of bioactive organic molecules and then to evaluate the bioactivity in vitro. The titanium plats were first cleaned by glow discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to free-terminal NH2 groups using glutaraldehyde as a coupling agent. Surface coverage by bound albumin was evaluated by SEM visualization. The results implied that the albumin was successfully grafted on to the titanium plates by the glow discharge technology, and this method offers the possibility of covalently linking selected molecules in order to guide and promote tissue healing that occurs during implant integration in bone and soft tissue. The argon-plasma treatment time was proportional to the surface roughness and degree of cleaning. They revealed that bombardment by energetic radicals and ions had occurred during plasma treatment. This implied that the plasma treatment did not lead to argon damage or a nitridation effect. Allylamine with terminal NH2 groups was successfully linked the with plasma-treated titanium plate. The binding energy shift of the N 1s peak was obvious. This revealed that allylamine was ionized by the plasma treatments, and that the dangling bond acted as a medium to link with albumin. Osteoblast-like MC3T3-E1 cells were cultured on the plasma-treated titanium plates for from 8 to 48 hours. Results showed that the osteoblast-like cells spread radically after 8 hours, and better adhesion were observed after 24 hours. This implied that the plasma-treated titanium surface possessed better bioactivity and biocompatibility than that without plasma modification.
Original languageTraditional Chinese
Pages (from-to)404-414
Number of pages11
Journal中華牙醫學雜誌(中文版)
Volume23
Issue number5
Publication statusPublished - 2004

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Allylamine
Titanium
Albumins
Plasmas
Glow discharges
Argon
Osteoblasts
Bioactivity
Tissue
Molecules
Dangling bonds
Nitridation
Coupling agents
Glutaral
Binding energy
Biocompatibility
Oxides
Cleaning
Bone
Adhesion

Keywords

  • titanium
  • plasma treatment
  • allylamine
  • albumin

Cite this

歐耿良(Keng-Liang O, 林耀楠(Yao-Nan L, 陳正雄(Cheng-Hsiung C, 張維仁(Wei-Jen C, 林哲堂(Che-Tong L, 謝松志(Sung-Chih H, ... 李勝揚(Sheng-Yang L (2004). 白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究. 中華牙醫學雜誌(中文版), 23(5), 404-414.

白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究. / 歐耿良(Keng-Liang Ou); 林耀楠(Yao-Nan Lin); 陳正雄(Cheng-Hsiung Chen); 張維仁(Wei-Jen Chang); 林哲堂(Che-Tong Ling); 謝松志(Sung-Chih Hsieh); 鄧乃嘉(Nai-Chia Jeng); 李勝揚(Sheng-Yang Lee).

In: 中華牙醫學雜誌(中文版), Vol. 23, No. 5, 2004, p. 404-414.

Research output: Contribution to journalArticle

歐耿良(Keng-LiangO, 林耀楠(Yao-NanL, 陳正雄(Cheng-HsiungC, 張維仁(Wei-JenC, 林哲堂(Che-TongL, 謝松志(Sung-ChihH, 鄧乃嘉(Nai-ChiaJ & 李勝揚(Sheng-YangL 2004, '白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究', 中華牙醫學雜誌(中文版), vol. 23, no. 5, pp. 404-414.
歐耿良(Keng-LiangO, 林耀楠(Yao-NanL, 陳正雄(Cheng-HsiungC, 張維仁(Wei-JenC, 林哲堂(Che-TongL, 謝松志(Sung-ChihH et al. 白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究. 中華牙醫學雜誌(中文版). 2004;23(5):404-414.
歐耿良(Keng-Liang Ou) ; 林耀楠(Yao-Nan Lin) ; 陳正雄(Cheng-Hsiung Chen) ; 張維仁(Wei-Jen Chang) ; 林哲堂(Che-Tong Ling) ; 謝松志(Sung-Chih Hsieh) ; 鄧乃嘉(Nai-Chia Jeng) ; 李勝揚(Sheng-Yang Lee). / 白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究. In: 中華牙醫學雜誌(中文版). 2004 ; Vol. 23, No. 5. pp. 404-414.
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title = "白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究",
abstract = "本研究利用電漿表面處理來活化鈦金屬表面以連接生物活化性白蛋白(albumin), 發展一植體表面處理技術。鈦金屬表面先以氫氣電漿清潔,接著以丙烯胺(allylamine)電漿處理,使經氫氣電漿清潔後的表面附著上胺基(-NH2),再以交鍵劑戊二醛(glutaraldehyde )連接固定白蛋白於低溫電漿處理後的鈦金屬植體。經掃描式電子顯微鏡及原子力顯微鏡觀察顯示經電漿解離的氬離子與原子具清潔及離子轟擊(ionbombardment)鈦金屬植體表面的功能。以低掠角X光繞射儀得知,經電漿處理後的鈦金屬植體繞射角度及相對繞射強度並無顯著移位及遞減,亦即具(002)優選繞射晶面的鈦金屬植體經電漿處理後其表面並無任何氬氣損傷及氮化效應產生,取而代之經電漿離子化的丙烯胺以胺基(-NH2)型態成功地附著於鈦金屬植體,此結果將有益於對白蛋白的固定。經X光光電子儀分析白蛋白吸附於丙烯胺電漿處理後的鈦金屬植體化學鍵結能的改變得證,其比無電漿處理的鈦金屬植體較易於披覆白蛋白。經SEM觀察類骨母細胞披覆於白蛋白鈦金屬圓片得知與計數附著鈦金屬上之細胞數量有正相關,且在相對細胞活性中也顯示經電漿處理披覆白蛋白的鈦金屬具較高的細胞活性。",
keywords = "鈦金屬, 電漿表面處理, 丙烯胺, 白蛋白, titanium, plasma treatment, allylamine, albumin",
author = "歐, {耿良(Keng-Liang Ou)} and 林, {耀楠(Yao-Nan Lin)} and 陳, {正雄(Cheng-Hsiung Chen)} and 張, {維仁(Wei-Jen Chang)} and 林, {哲堂(Che-Tong Ling)} and 謝, {松志(Sung-Chih Hsieh)} and 鄧, {乃嘉(Nai-Chia Jeng)} and 李, {勝揚(Sheng-Yang Lee)}",
year = "2004",
language = "繁體中文",
volume = "23",
pages = "404--414",
journal = "中華牙醫學雜誌(中文版)",
issn = "1010-3287",
publisher = "中華牙醫學會",
number = "5",

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TY - JOUR

T1 - 白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究

AU - 歐, 耿良(Keng-Liang Ou)

AU - 林, 耀楠(Yao-Nan Lin)

AU - 陳, 正雄(Cheng-Hsiung Chen)

AU - 張, 維仁(Wei-Jen Chang)

AU - 林, 哲堂(Che-Tong Ling)

AU - 謝, 松志(Sung-Chih Hsieh)

AU - 鄧, 乃嘉(Nai-Chia Jeng)

AU - 李, 勝揚(Sheng-Yang Lee)

PY - 2004

Y1 - 2004

N2 - 本研究利用電漿表面處理來活化鈦金屬表面以連接生物活化性白蛋白(albumin), 發展一植體表面處理技術。鈦金屬表面先以氫氣電漿清潔,接著以丙烯胺(allylamine)電漿處理,使經氫氣電漿清潔後的表面附著上胺基(-NH2),再以交鍵劑戊二醛(glutaraldehyde )連接固定白蛋白於低溫電漿處理後的鈦金屬植體。經掃描式電子顯微鏡及原子力顯微鏡觀察顯示經電漿解離的氬離子與原子具清潔及離子轟擊(ionbombardment)鈦金屬植體表面的功能。以低掠角X光繞射儀得知,經電漿處理後的鈦金屬植體繞射角度及相對繞射強度並無顯著移位及遞減,亦即具(002)優選繞射晶面的鈦金屬植體經電漿處理後其表面並無任何氬氣損傷及氮化效應產生,取而代之經電漿離子化的丙烯胺以胺基(-NH2)型態成功地附著於鈦金屬植體,此結果將有益於對白蛋白的固定。經X光光電子儀分析白蛋白吸附於丙烯胺電漿處理後的鈦金屬植體化學鍵結能的改變得證,其比無電漿處理的鈦金屬植體較易於披覆白蛋白。經SEM觀察類骨母細胞披覆於白蛋白鈦金屬圓片得知與計數附著鈦金屬上之細胞數量有正相關,且在相對細胞活性中也顯示經電漿處理披覆白蛋白的鈦金屬具較高的細胞活性。

AB - 本研究利用電漿表面處理來活化鈦金屬表面以連接生物活化性白蛋白(albumin), 發展一植體表面處理技術。鈦金屬表面先以氫氣電漿清潔,接著以丙烯胺(allylamine)電漿處理,使經氫氣電漿清潔後的表面附著上胺基(-NH2),再以交鍵劑戊二醛(glutaraldehyde )連接固定白蛋白於低溫電漿處理後的鈦金屬植體。經掃描式電子顯微鏡及原子力顯微鏡觀察顯示經電漿解離的氬離子與原子具清潔及離子轟擊(ionbombardment)鈦金屬植體表面的功能。以低掠角X光繞射儀得知,經電漿處理後的鈦金屬植體繞射角度及相對繞射強度並無顯著移位及遞減,亦即具(002)優選繞射晶面的鈦金屬植體經電漿處理後其表面並無任何氬氣損傷及氮化效應產生,取而代之經電漿離子化的丙烯胺以胺基(-NH2)型態成功地附著於鈦金屬植體,此結果將有益於對白蛋白的固定。經X光光電子儀分析白蛋白吸附於丙烯胺電漿處理後的鈦金屬植體化學鍵結能的改變得證,其比無電漿處理的鈦金屬植體較易於披覆白蛋白。經SEM觀察類骨母細胞披覆於白蛋白鈦金屬圓片得知與計數附著鈦金屬上之細胞數量有正相關,且在相對細胞活性中也顯示經電漿處理披覆白蛋白的鈦金屬具較高的細胞活性。

KW - 鈦金屬

KW - 電漿表面處理

KW - 丙烯胺

KW - 白蛋白

KW - titanium

KW - plasma treatment

KW - allylamine

KW - albumin

M3 - 文章

VL - 23

SP - 404

EP - 414

JO - 中華牙醫學雜誌(中文版)

JF - 中華牙醫學雜誌(中文版)

SN - 1010-3287

IS - 5

ER -