In-vitro recording of adult zebrafish heart electrocardiogram - A platform for pharmacological testing

Chia Ti Tsai, Cho Kai Wu, Fu Tien Chiang, Chuen Den Tseng, Jen Kuang Lee, Chih Chieh Yu, Yi Chih Wang, Ling Ping Lai, Jiunn Lee Lin, Juey Jen Hwang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Recently zebrafish has become a powerful vertebrate model system for cardiac arrhythmia and another advantage is that the heart could be rapidly excised for in-vitro electrophysiological recording. Methods: We made direct in-vitro recordings of adult zebrafish heart ECG using the microelectrode and tested the effects of various drugs on zebrafish heart ECG. Results: The QT interval change in the ECG was verified by optically mapped action potential duration (APD). The mean in-vitro heart rate (HR) of the adult zebrafish was 118 ± 22. beats/min. The mean QT interval was 312 ± 36. ms and mean HR corrected QT interval (QTc) 439 ± 39. ms. The mean optically mapped APD was 308 ± 30. ms, which was close to the QT interval in ECG (p = 0.815). We found that sympathomimetic drug isoproterenol increased HR, whereas L-type calcium channel blocker verapamil decreased HR. Sodium channel blocker quinidine and L-type calcium channel activator BayK8644 prolonged QTc interval in a dose-dependent manner (515 ± 24 ms and 519 ± 27. ms, respectively, both p < 0.01). The APD was also prolonged accordingly. Both rapidly and slowly activating delayed rectifier potassium channel (IKr and IKs) blockers E4031 and chromanol 293B, respectively, also prolonged QTc interval in a dose-dependent manner (523 ± 25 ms and 529 ± 27 ms, respectively, both p < 0.01). Quinidine, E4031 and chromanol 293B also decreased HR. Conclusions: Direct in-vitro recording of adult zebrafish heart is an efficient platform for test of drugs which exert electrophysiological effects on cardiomyocytes, and perturbation of ionic channels that are responsible for human long QT syndrome also modulates QT interval in adult zebrafish heart.

Original languageEnglish
Pages (from-to)1963-1967
Number of pages5
JournalClinica Chimica Acta
Volume412
Issue number21-22
DOIs
Publication statusPublished - Oct 9 2011
Externally publishedYes

Fingerprint

Zebrafish
Electrocardiography
Pharmacology
Heart Rate
Testing
Action Potentials
L-Type Calcium Channels
Quinidine
Calcium Channel Agonists
Sodium Channel Blockers
Long QT Syndrome
Sympathomimetics
Calcium Channel Blockers
Microelectrodes
Verapamil
Ion Channels
Isoproterenol
Cardiac Myocytes
Pharmaceutical Preparations
Vertebrates

Keywords

  • Electrocardiogram
  • In-vitro recording
  • Long QT
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Tsai, C. T., Wu, C. K., Chiang, F. T., Tseng, C. D., Lee, J. K., Yu, C. C., ... Hwang, J. J. (2011). In-vitro recording of adult zebrafish heart electrocardiogram - A platform for pharmacological testing. Clinica Chimica Acta, 412(21-22), 1963-1967. https://doi.org/10.1016/j.cca.2011.07.002

In-vitro recording of adult zebrafish heart electrocardiogram - A platform for pharmacological testing. / Tsai, Chia Ti; Wu, Cho Kai; Chiang, Fu Tien; Tseng, Chuen Den; Lee, Jen Kuang; Yu, Chih Chieh; Wang, Yi Chih; Lai, Ling Ping; Lin, Jiunn Lee; Hwang, Juey Jen.

In: Clinica Chimica Acta, Vol. 412, No. 21-22, 09.10.2011, p. 1963-1967.

Research output: Contribution to journalArticle

Tsai, CT, Wu, CK, Chiang, FT, Tseng, CD, Lee, JK, Yu, CC, Wang, YC, Lai, LP, Lin, JL & Hwang, JJ 2011, 'In-vitro recording of adult zebrafish heart electrocardiogram - A platform for pharmacological testing', Clinica Chimica Acta, vol. 412, no. 21-22, pp. 1963-1967. https://doi.org/10.1016/j.cca.2011.07.002
Tsai, Chia Ti ; Wu, Cho Kai ; Chiang, Fu Tien ; Tseng, Chuen Den ; Lee, Jen Kuang ; Yu, Chih Chieh ; Wang, Yi Chih ; Lai, Ling Ping ; Lin, Jiunn Lee ; Hwang, Juey Jen. / In-vitro recording of adult zebrafish heart electrocardiogram - A platform for pharmacological testing. In: Clinica Chimica Acta. 2011 ; Vol. 412, No. 21-22. pp. 1963-1967.
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