In vitro Penetration and in vivo distribution of honokiol into the intervertebral disc in rat

Chia Hsien Chen, Chang-Jung Chiang, Lien Chen Wu, Chih Hong Yang, Yi-Jie Kuo, Tung Hu Tsai

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Honokiol is a potential candidate for the treatment of intervertebral disc (IVD) degeneration. In this study, we develop in vitro and in vivo methods to detect the distribution of honokiol in intervertebral discs using high-performance liquid chromatography. A rat tail disc was used for both experimental models. For the in vivo animal experiment, blood samples and tail discs were collected at 15, 30, 60, 120 and 240 min after honokiol administration (30 mg/kg, i.v.). The analyte was separated by a mobile phase of methanol and 10 mM NaH2PO4 buffer at pH 2.8 (78:22, v/v) and pumped through a reversed-phase analytical column (250 × 4.6 mm, particle size 5 μm) at room temperature. The in vitro experimental results demonstrated that honokiol diffused into the intervertebral disc and was concentration-dependent. The active concentration is obtained for the therapeutic level at 15 and 30 min after honokiol administration in the in vivo model.

Original languageEnglish
Pages (from-to)1297-1302
Number of pages6
JournalAnalytical Sciences
Volume31
Issue number12
DOIs
Publication statusPublished - 2015

Keywords

  • Honokiol
  • Hypoxia inducible factors inhibitor
  • Intervertebral disc
  • Pharmacokinetics
  • Traditional Chinese medicine

ASJC Scopus subject areas

  • Analytical Chemistry

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