In vitro hepatic differentiation of human mesenchymal stem cells

Kuan Der Lee, Tom Kwang Chun Kuo, Jacqueline Whang-Peng, Yu Fen Chung, Ching Tai Lin, Shiu Huey Chou, Jim Ray Chen, Yi Peng Chen, Oscar Kuang Sheng Lee

Research output: Contribution to journalArticle

710 Citations (Scopus)

Abstract

This study examined whether mesenchymal stem cells (MSCs), which are stem cells originated from embryonic mesoderm, are able to differentiate into functional hepatocyte-like cells in vitro. MSCs were isolated from human bone marrow and umbilical cord blood, and the surface phenotype and the mesodermal multilineage differentiation potentials of these cells were characterized and tested. To effectively induce hepatic differentiation, we designed a novel 2-step protocol with the use of hepatocyte growth factor and oncostatin M. After 4 weeks of induction, cuboidal morphology, which is characteristic of hepatocytes, was observed, and cells also expressed marker genes specific of liver cells in a time-dependent manner. Differentiated cells further demonstrated in vitro functions characteristic of liver cells, including albumin production, glycogen storage, urea secretion, uptake of low-density lipoprotein, and phenobarbital-inducible cytochrome P450 activity. In conclusion, human MSCs from different sources are able to differentiate into functional hepatocyte-like cells and, hence, may serve as a cell source for tissue engineering and cell therapy of hepatic tissues. Furthermore, the broad differentiation potential of MSCs indicates that a revision of the definition may be required.

Original languageEnglish
Pages (from-to)1275-1284
Number of pages10
JournalHepatology
Volume40
Issue number6
DOIs
Publication statusPublished - Dec 2004
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Liver
Hepatocytes
Cell- and Tissue-Based Therapy
Oncostatin M
Hepatocyte Growth Factor
Mesoderm
Tissue Engineering
Embryonic Stem Cells
Phenobarbital
In Vitro Techniques
Glycogen
Fetal Blood
LDL Lipoproteins
Cytochrome P-450 Enzyme System
Urea
Cell Differentiation
Albumins
Bone Marrow
Phenotype

ASJC Scopus subject areas

  • Hepatology

Cite this

Lee, K. D., Kuo, T. K. C., Whang-Peng, J., Chung, Y. F., Lin, C. T., Chou, S. H., ... Lee, O. K. S. (2004). In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology, 40(6), 1275-1284. https://doi.org/10.1002/hep.20469

In vitro hepatic differentiation of human mesenchymal stem cells. / Lee, Kuan Der; Kuo, Tom Kwang Chun; Whang-Peng, Jacqueline; Chung, Yu Fen; Lin, Ching Tai; Chou, Shiu Huey; Chen, Jim Ray; Chen, Yi Peng; Lee, Oscar Kuang Sheng.

In: Hepatology, Vol. 40, No. 6, 12.2004, p. 1275-1284.

Research output: Contribution to journalArticle

Lee, KD, Kuo, TKC, Whang-Peng, J, Chung, YF, Lin, CT, Chou, SH, Chen, JR, Chen, YP & Lee, OKS 2004, 'In vitro hepatic differentiation of human mesenchymal stem cells', Hepatology, vol. 40, no. 6, pp. 1275-1284. https://doi.org/10.1002/hep.20469
Lee, Kuan Der ; Kuo, Tom Kwang Chun ; Whang-Peng, Jacqueline ; Chung, Yu Fen ; Lin, Ching Tai ; Chou, Shiu Huey ; Chen, Jim Ray ; Chen, Yi Peng ; Lee, Oscar Kuang Sheng. / In vitro hepatic differentiation of human mesenchymal stem cells. In: Hepatology. 2004 ; Vol. 40, No. 6. pp. 1275-1284.
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