In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes

Chien-Chin Yang, Yi-Hong Chen, Hsiu-Hui Tsai, Chih-Hung Huang, Tzu-Wen Huang, Hsiuh-Wei Chen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Single-stranded gaps at the 3′ ends of Streptomyces linear replicons are patched by DNA synthesis primed by terminal proteins (TP) during replication. We devised an in vitro system that specifically incorporated dCMP, the first nucleotide at the 5́ ends, onto a threonine residue of the TP of Streptomyces coelicolor. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)7959-7961
Number of pages3
JournalApplied and Environmental Microbiology
Volume72
Issue number12
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Chromosomes
  • Phosphates
  • Proteins
  • In vitro deoxynucleotidylation
  • Streptomyces linear chromosomes
  • Threonine residues
  • Bacteria
  • bacterial protein
  • deoxycytidine phosphate
  • nucleotide
  • terminal protein
  • threonine
  • amino acid
  • bacterium
  • chromosome
  • DNA
  • protein
  • article
  • bacterial chromosome
  • chromosome structure
  • controlled study
  • deoxynucleotidylation
  • DNA flanking region
  • DNA replication
  • DNA synthesis
  • in vitro study
  • nonhuman
  • protein modification
  • replicon
  • Streptomyces
  • Streptomyces coelicolor
  • Bacterial Proteins
  • Chromosomes, Bacterial
  • Deoxycytidine Monophosphate
  • DNA Replication
  • DNA, Bacterial
  • Phosphorus Radioisotopes

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