In vitro cell behavior of osteoblasts on pyrost bone substitute

Yang Hwei Tsuang, Feng Huei Lin, Jui Sheng Sun, Yi Shiong Hang, Hwa Chang Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute. Methods: By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope. Results: The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes. Conclusions: These results demonstrated that Pyrost can form a physicochemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalAnatomical Record
Volume247
Issue number2
DOIs
Publication statusPublished - Feb 1997
Externally publishedYes

Fingerprint

Bone Substitutes
osteoblasts
Osteoblasts
bone
cells
adhesion
substrate
Pyrost
In Vitro Techniques
bone substitutes
pseudopodia
biocompatible materials
Pseudopodia
orthopedics
Biocompatible Materials
Growth
scanning electron microscopes
electron
Orthopedics
Suspensions

Keywords

  • adhesion
  • osteoblasts
  • pyrost bone
  • spreading

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

In vitro cell behavior of osteoblasts on pyrost bone substitute. / Tsuang, Yang Hwei; Lin, Feng Huei; Sun, Jui Sheng; Hang, Yi Shiong; Liu, Hwa Chang.

In: Anatomical Record, Vol. 247, No. 2, 02.1997, p. 164-169.

Research output: Contribution to journalArticle

Tsuang, Yang Hwei ; Lin, Feng Huei ; Sun, Jui Sheng ; Hang, Yi Shiong ; Liu, Hwa Chang. / In vitro cell behavior of osteoblasts on pyrost bone substitute. In: Anatomical Record. 1997 ; Vol. 247, No. 2. pp. 164-169.
@article{51498c37fba04347b7e073a28ba1ff6a,
title = "In vitro cell behavior of osteoblasts on pyrost bone substitute",
abstract = "Background: Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute. Methods: By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope. Results: The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes. Conclusions: These results demonstrated that Pyrost can form a physicochemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts.",
keywords = "adhesion, osteoblasts, pyrost bone, spreading",
author = "Tsuang, {Yang Hwei} and Lin, {Feng Huei} and Sun, {Jui Sheng} and Hang, {Yi Shiong} and Liu, {Hwa Chang}",
year = "1997",
month = "2",
doi = "10.1002/(SICI)1097-0185(199702)247:2<164::AID-AR2>3.0.CO;2-Y",
language = "English",
volume = "247",
pages = "164--169",
journal = "Anatomical Record",
issn = "0003-276X",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - In vitro cell behavior of osteoblasts on pyrost bone substitute

AU - Tsuang, Yang Hwei

AU - Lin, Feng Huei

AU - Sun, Jui Sheng

AU - Hang, Yi Shiong

AU - Liu, Hwa Chang

PY - 1997/2

Y1 - 1997/2

N2 - Background: Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute. Methods: By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope. Results: The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes. Conclusions: These results demonstrated that Pyrost can form a physicochemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts.

AB - Background: Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute. Methods: By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope. Results: The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes. Conclusions: These results demonstrated that Pyrost can form a physicochemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts.

KW - adhesion

KW - osteoblasts

KW - pyrost bone

KW - spreading

UR - http://www.scopus.com/inward/record.url?scp=0031018745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031018745&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0185(199702)247:2<164::AID-AR2>3.0.CO;2-Y

DO - 10.1002/(SICI)1097-0185(199702)247:2<164::AID-AR2>3.0.CO;2-Y

M3 - Article

C2 - 9025995

AN - SCOPUS:0031018745

VL - 247

SP - 164

EP - 169

JO - Anatomical Record

JF - Anatomical Record

SN - 0003-276X

IS - 2

ER -