In vitro antimicrobial and antipro-inflammation potential of honokiol and magnolol against oral pathogens and macrophages

Kuo Chou Chiu, Yin Hwa Shih, Tong Hong Wang, Wan Chen Lan, Po Jung Li, Hong Syu Jhuang, Shih Min Hsia, Yen Wen Shen, Michael Yuan-Chien Chen, Tzong Ming Shieh

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Background/purpose: Honokiol and magnolol are natural components isolated from Magnolia bark that is used in traditional Chinese and Japanese herbal medicine. These two isomers are used as a component of dietary supplements and cosmetic products. In this study, we investigated the antimicrobial effect of honokiol and magnolol on pathogens causing oral diseases, their mechanism of action in biofilm formation and drug resistance of oral pathogens, and inflammatory regulation in mammalian cells. Methods: We determined the minimum inhibitory concentration and minimum bactericidal concentration of honokiol and magnolol, and their stability at different temperatures and pH. We also evaluated their effect on biofilm formation, antibiotic-resistance gene expression in MRSA, and pro-inflammatory gene expression in mammalian cells. Results: Honokiol showed better antimicrobial activity than magnolol. Both honokiol and magnolol showed stable bacterial inhibitory activity over a wide range of temperature and pH, reduced biofilm formation, and antibiotic resistance in oral pathogens. The biofilm formation- and antibiotic resistance-related gene expression was consistent with the respective phenotypes. Furthermore, these two isomers repressed the expression of pro-inflammatory genes in RAW264.7 cells. Conclusion: Our study provides evidence of the potential application of honokiol and magnolol in dental medicine to cure or prevent oral diseases.

Original languageEnglish
Pages (from-to)827-837
Number of pages11
JournalJournal of the Formosan Medical Association
Issue number2
Publication statusPublished - Feb 2021


  • Antibiotic resistance
  • Biofilm
  • Honokiol
  • Magnolol
  • Pro-inflammatory

ASJC Scopus subject areas

  • Medicine(all)


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