In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Chih Hung Lin, Chia Lang Fang, Saleh A. Al-Suwayeh, Shih Yun Yang, Jia You Fang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim:To investigate the in vitro and in vivo percutaneous absorption of seleno-L-methionine (Se-L-M), an ultraviolet (UV)-protecting agent, from aqueous solutions.Methods:Aqueous solutions of Se-L-M were prepared in p H 4, 8, and 10.8 buffers. The p H 8 buffer contained 30% glycerol, propylene glycol (PG) and polyethylene glycol (PEG) 400. The in vitro skin permeation of Se-L-M via porcine skin and nude mouse skin was measured and compared using Franz diffusion cells. The in vivo skin tolerance study was performed, which examined transepidermal water loss (TEWL), skin p H and erythema.Results:In the excised porcine skin, the flux was 0.1, 11.4 and 8.2 gcm 2 h 1 for the p H 4, 8, and 10.8 buffers, respectively. A linear correlation between the flux and skin deposition was determined. According to permeation across skin with different treatments (stripping, delipidation, and ethanol treatments), it was determined that the intracellular route comprised the predominant pathway for Se-L-M permeation from p H 8 buffer. Aqueous solutions of seleno-DL-methionine (Se-DL-M), selenium sulfide and selenium-containing quantum dot nanoparticles were also used as donor systems. The DL form showed a lower flux (7.0 vs 11.4 gcm 2 h 1) and skin uptake (23.4 vs 47.3 g/g) as compared to the L form, indicating stereoselective permeation of this compound. There was no or only negligible permeation of selenium sulfide and quantum dots into and across the skin. With in vivo topical application for 4 and 8 h, the skin deposition of Se-L-M was about 7 g/g, and values were comparable to each other. The topical application of Se-L-M for up to 5 d did not caused apparent skin irritation. However, slight inflammation of the dermis was noted according to the histopathological examination.Conclusion:Se-L-M was readily absorbed by the skin in both the in vitro and in vivo experiments. The established profiles of Se-L-M skin absorption will be helpful in developing topical products of this compound.

Original languageEnglish
Pages (from-to)1181-1190
Number of pages10
JournalActa Pharmacologica Sinica
Volume32
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Skin Absorption
Selenium
Methionine
Antioxidants
Skin
Buffers
Quantum Dots
In Vitro Techniques
Swine
L Forms
Propylene Glycol
Erythema
Dermis
Nude Mice
Nanoparticles
Glycerol

Keywords

  • percutaneous absorption
  • selenium
  • seleno-L-methionine
  • skin
  • topical delivery

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species. / Lin, Chih Hung; Fang, Chia Lang; Al-Suwayeh, Saleh A.; Yang, Shih Yun; Fang, Jia You.

In: Acta Pharmacologica Sinica, Vol. 32, No. 9, 09.2011, p. 1181-1190.

Research output: Contribution to journalArticle

Lin, Chih Hung ; Fang, Chia Lang ; Al-Suwayeh, Saleh A. ; Yang, Shih Yun ; Fang, Jia You. / In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species. In: Acta Pharmacologica Sinica. 2011 ; Vol. 32, No. 9. pp. 1181-1190.
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AU - Lin, Chih Hung

AU - Fang, Chia Lang

AU - Al-Suwayeh, Saleh A.

AU - Yang, Shih Yun

AU - Fang, Jia You

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N2 - Aim:To investigate the in vitro and in vivo percutaneous absorption of seleno-L-methionine (Se-L-M), an ultraviolet (UV)-protecting agent, from aqueous solutions.Methods:Aqueous solutions of Se-L-M were prepared in p H 4, 8, and 10.8 buffers. The p H 8 buffer contained 30% glycerol, propylene glycol (PG) and polyethylene glycol (PEG) 400. The in vitro skin permeation of Se-L-M via porcine skin and nude mouse skin was measured and compared using Franz diffusion cells. The in vivo skin tolerance study was performed, which examined transepidermal water loss (TEWL), skin p H and erythema.Results:In the excised porcine skin, the flux was 0.1, 11.4 and 8.2 gcm 2 h 1 for the p H 4, 8, and 10.8 buffers, respectively. A linear correlation between the flux and skin deposition was determined. According to permeation across skin with different treatments (stripping, delipidation, and ethanol treatments), it was determined that the intracellular route comprised the predominant pathway for Se-L-M permeation from p H 8 buffer. Aqueous solutions of seleno-DL-methionine (Se-DL-M), selenium sulfide and selenium-containing quantum dot nanoparticles were also used as donor systems. The DL form showed a lower flux (7.0 vs 11.4 gcm 2 h 1) and skin uptake (23.4 vs 47.3 g/g) as compared to the L form, indicating stereoselective permeation of this compound. There was no or only negligible permeation of selenium sulfide and quantum dots into and across the skin. With in vivo topical application for 4 and 8 h, the skin deposition of Se-L-M was about 7 g/g, and values were comparable to each other. The topical application of Se-L-M for up to 5 d did not caused apparent skin irritation. However, slight inflammation of the dermis was noted according to the histopathological examination.Conclusion:Se-L-M was readily absorbed by the skin in both the in vitro and in vivo experiments. The established profiles of Se-L-M skin absorption will be helpful in developing topical products of this compound.

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