In vitro and in vivo methods to measure the ceftriaxone distribution into the rat tail intervertebral disc

Chia Chun Lin, Yu Tse Wu, Jiin Cherng Yen, Chang Jung Chiang, Yang Hwei Tsuang, Tung Hu Tsai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To characterize the pathway of ceftriaxone penetration into intervertebral discs, rat-tail discs were collected and immersed in a ceftriaxone solution for an in vitro study. For this in vivo experiment, plasma and tail discs were collected 30 min after ceftriaxone administration (463.2 mg/kg, equal to 500 mg/kg disodium salt, iv). The ceftriaxone levels in the plasma and discs were determined with HPLC by a reversed-phase C18 column and a mobile phase of methanol and a 10 mM K2HPO4 solution (pH 6.7) (21:79, v/v). The results indicate that ceftriaxone was undetectable by the in vitro model. The concentrations of ceftriaxone in plasma and in intervertebral discs were 147.4 ± 21.2 and 4.0 ± 1.7 mg/mL (as disodium salt), respectively. Our results suggest that a sufficient blood supply is required for the passive diffusion of ceftriaxone into intervertebral discs. The concentration ratio of ceftriaxone in intervertebral disc-to-plasma was about 2.7% at 30 min after ceftriaxone disodium administration (500 mg/kg, iv).

Original languageEnglish
Pages (from-to)979-982
Number of pages4
JournalAnalytical Sciences
Volume26
Issue number9
DOIs
Publication statusPublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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