In-vitro activity of tigecycline against clinical isolates of Acinetobacter baumannii in Taiwan determined by the broth microdilution and disk diffusion methods

Chun Hsing Liao, Hsiang Chi Kung, Gwo Jong Hsu, Po Liang Lu, Yung Ching Liu, Chih Ming Chen, Chun Ming Lee, Wu Sun, Tsrang Neng Jang, Ping Cherng Chiang, Yu Jen Cheng, Hsiu Chen Lin, Zhi Yuan Shi, Lih Shinn Wang, Yin Ching Chuang, Shih Ming Tsao, Chin Te Lu, Jien Wei Liu, Cheng Hua Huang, Po Ren Hsueh

Research output: Contribution to journalArticle

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Abstract

A total of 393 isolates of A. baumannii were collected from patients treated at 19 teaching hospitals in Taiwan. Minimum inhibitory concentrations (MICs) and inhibitory zone diameters for tigecycline were determined by the broth microdilution method and the disk diffusion method, respectively. The MIC results were interpreted using the US FDA tigecycline susceptibility breakpoints for Enterobacteriaceae (susceptible [S] ≤2 μg/mL; intermediate [I] 4 μg/mL; resistant [R] ≥8 μg/mL). The disk diffusion results were interpreted by criteria recommended by Jones et al. (S ≥16 mm; I 13-15 mm; R ≤12 mm) and also by those recommended by the US FDA for Enterobacteriaceae (S ≥19 mm; I 15-18 mm; R ≤14 mm). The percentages of susceptible, intermediate and resistant isolates determined by the broth microdilution method were 80.9%, 12.2%, and 6.9%, respectively. The rates of susceptible, intermediate and resistant isolates by the disk diffusion method using the criteria of Jones et al. were 88.3%, 9.9% and 1.8% and using the US FDA criteria were 44.0%, 51.7% and 4.3%. Using the criteria recommended by Jones et al., the total error rate of the disk diffusion method in comparison with the broth microdilution method was 14.2% (56/393). For routine susceptibility testing of tigecycline against A. baumannii the broth microdilution method, not the disk diffusion method, should be used due to the poor correlation of results between these two methods interpreted either by the Jones et al. or US FDA criteria.

Original languageEnglish
Pages (from-to)S192-S196
JournalInternational Journal of Antimicrobial Agents
Volume32
Issue numberSUPPL. 3
DOIs
Publication statusPublished - Nov 2008
Externally publishedYes

Fingerprint

Acinetobacter baumannii
Taiwan
Microbial Sensitivity Tests
Enterobacteriaceae
tigecycline
In Vitro Techniques
Teaching Hospitals

Keywords

  • Acinetobacter baumannii
  • Broth microdilution
  • Disk diffusion
  • Taiwan
  • Tigecycline

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

In-vitro activity of tigecycline against clinical isolates of Acinetobacter baumannii in Taiwan determined by the broth microdilution and disk diffusion methods. / Liao, Chun Hsing; Kung, Hsiang Chi; Hsu, Gwo Jong; Lu, Po Liang; Liu, Yung Ching; Chen, Chih Ming; Lee, Chun Ming; Sun, Wu; Jang, Tsrang Neng; Chiang, Ping Cherng; Cheng, Yu Jen; Lin, Hsiu Chen; Shi, Zhi Yuan; Wang, Lih Shinn; Chuang, Yin Ching; Tsao, Shih Ming; Lu, Chin Te; Liu, Jien Wei; Huang, Cheng Hua; Hsueh, Po Ren.

In: International Journal of Antimicrobial Agents, Vol. 32, No. SUPPL. 3, 11.2008, p. S192-S196.

Research output: Contribution to journalArticle

Liao, CH, Kung, HC, Hsu, GJ, Lu, PL, Liu, YC, Chen, CM, Lee, CM, Sun, W, Jang, TN, Chiang, PC, Cheng, YJ, Lin, HC, Shi, ZY, Wang, LS, Chuang, YC, Tsao, SM, Lu, CT, Liu, JW, Huang, CH & Hsueh, PR 2008, 'In-vitro activity of tigecycline against clinical isolates of Acinetobacter baumannii in Taiwan determined by the broth microdilution and disk diffusion methods', International Journal of Antimicrobial Agents, vol. 32, no. SUPPL. 3, pp. S192-S196. https://doi.org/10.1016/S0924-8579(08)70027-X
Liao, Chun Hsing ; Kung, Hsiang Chi ; Hsu, Gwo Jong ; Lu, Po Liang ; Liu, Yung Ching ; Chen, Chih Ming ; Lee, Chun Ming ; Sun, Wu ; Jang, Tsrang Neng ; Chiang, Ping Cherng ; Cheng, Yu Jen ; Lin, Hsiu Chen ; Shi, Zhi Yuan ; Wang, Lih Shinn ; Chuang, Yin Ching ; Tsao, Shih Ming ; Lu, Chin Te ; Liu, Jien Wei ; Huang, Cheng Hua ; Hsueh, Po Ren. / In-vitro activity of tigecycline against clinical isolates of Acinetobacter baumannii in Taiwan determined by the broth microdilution and disk diffusion methods. In: International Journal of Antimicrobial Agents. 2008 ; Vol. 32, No. SUPPL. 3. pp. S192-S196.
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abstract = "A total of 393 isolates of A. baumannii were collected from patients treated at 19 teaching hospitals in Taiwan. Minimum inhibitory concentrations (MICs) and inhibitory zone diameters for tigecycline were determined by the broth microdilution method and the disk diffusion method, respectively. The MIC results were interpreted using the US FDA tigecycline susceptibility breakpoints for Enterobacteriaceae (susceptible [S] ≤2 μg/mL; intermediate [I] 4 μg/mL; resistant [R] ≥8 μg/mL). The disk diffusion results were interpreted by criteria recommended by Jones et al. (S ≥16 mm; I 13-15 mm; R ≤12 mm) and also by those recommended by the US FDA for Enterobacteriaceae (S ≥19 mm; I 15-18 mm; R ≤14 mm). The percentages of susceptible, intermediate and resistant isolates determined by the broth microdilution method were 80.9{\%}, 12.2{\%}, and 6.9{\%}, respectively. The rates of susceptible, intermediate and resistant isolates by the disk diffusion method using the criteria of Jones et al. were 88.3{\%}, 9.9{\%} and 1.8{\%} and using the US FDA criteria were 44.0{\%}, 51.7{\%} and 4.3{\%}. Using the criteria recommended by Jones et al., the total error rate of the disk diffusion method in comparison with the broth microdilution method was 14.2{\%} (56/393). For routine susceptibility testing of tigecycline against A. baumannii the broth microdilution method, not the disk diffusion method, should be used due to the poor correlation of results between these two methods interpreted either by the Jones et al. or US FDA criteria.",
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AU - Liao, Chun Hsing

AU - Kung, Hsiang Chi

AU - Hsu, Gwo Jong

AU - Lu, Po Liang

AU - Liu, Yung Ching

AU - Chen, Chih Ming

AU - Lee, Chun Ming

AU - Sun, Wu

AU - Jang, Tsrang Neng

AU - Chiang, Ping Cherng

AU - Cheng, Yu Jen

AU - Lin, Hsiu Chen

AU - Shi, Zhi Yuan

AU - Wang, Lih Shinn

AU - Chuang, Yin Ching

AU - Tsao, Shih Ming

AU - Lu, Chin Te

AU - Liu, Jien Wei

AU - Huang, Cheng Hua

AU - Hsueh, Po Ren

PY - 2008/11

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N2 - A total of 393 isolates of A. baumannii were collected from patients treated at 19 teaching hospitals in Taiwan. Minimum inhibitory concentrations (MICs) and inhibitory zone diameters for tigecycline were determined by the broth microdilution method and the disk diffusion method, respectively. The MIC results were interpreted using the US FDA tigecycline susceptibility breakpoints for Enterobacteriaceae (susceptible [S] ≤2 μg/mL; intermediate [I] 4 μg/mL; resistant [R] ≥8 μg/mL). The disk diffusion results were interpreted by criteria recommended by Jones et al. (S ≥16 mm; I 13-15 mm; R ≤12 mm) and also by those recommended by the US FDA for Enterobacteriaceae (S ≥19 mm; I 15-18 mm; R ≤14 mm). The percentages of susceptible, intermediate and resistant isolates determined by the broth microdilution method were 80.9%, 12.2%, and 6.9%, respectively. The rates of susceptible, intermediate and resistant isolates by the disk diffusion method using the criteria of Jones et al. were 88.3%, 9.9% and 1.8% and using the US FDA criteria were 44.0%, 51.7% and 4.3%. Using the criteria recommended by Jones et al., the total error rate of the disk diffusion method in comparison with the broth microdilution method was 14.2% (56/393). For routine susceptibility testing of tigecycline against A. baumannii the broth microdilution method, not the disk diffusion method, should be used due to the poor correlation of results between these two methods interpreted either by the Jones et al. or US FDA criteria.

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