Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

Ming Hua Ho, Mei Hsiu Liao, Yi Ling Lin, Chien Hao Lai, Pei I. Lin, Ruei Ming Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Osteoblast maturation plays a key role in regulating osteogenesis. Electrospun nanofibrous products were reported to possess a high surface area and porosity. In this study, we developed chitosan nanofibers and examined the effects of nanofibrous scaffolds on osteoblast maturation and the possible mechanisms. Macro- and micro observations of the chitosan nanofibers revealed that these nanoproducts had a flat surface and well-distributed fibers with nanoscale diameters. Mouse osteoblasts were able to attach onto the chitosan nanofiber scaffolds, and the scaffolds degraded in a time-dependent manner. Analysis by scanning electron microscopy further showed mouse osteoblasts adhered onto the scaffolds along the nanofibers, and cell-cell communication was also detected. Mouse osteoblasts grew much better on chitosan nanofiber scaffolds than on chitosan films. In addition, human osteoblasts were able to adhere and grow on the chitosan nanofiber scaffolds. Interestingly, culturing human osteoblasts on chitosan nanofiber scaffolds time-dependently increased DNA replication and cell proliferation. In parallel, administration of human osteoblasts onto chitosan nanofibers significantly induced osteopontin, osteocalcin, and alkaline phosphatase (ALP) messenger (m)RNA expression. As to the mechanism, chitosan nanofibers triggered runt-related transcription factor 2 mRNA and protein syntheses. Consequently, results of ALP-, alizarin red-, and von Kossa-staining analyses showed that chitosan nanofibers improved osteoblast mineralization. Taken together, results of this study demonstrate that chitosan nanofibers can stimulate osteoblast proliferation and maturation via runt-related transcription factor 2-mediated regulation of osteoblast-associated osteopontin, osteocalcin, and ALP gene expression.

Original languageEnglish
Pages (from-to)4293-4304
Number of pages12
JournalInternational Journal of Nanomedicine
Volume9
DOIs
Publication statusPublished - 2014

Fingerprint

Nanofibers
Osteoblasts
Chitosan
Scaffolds
Phosphatases
Alkaline Phosphatase
Osteopontin
Transcription factors
Osteocalcin
Transcription Factors
Alizarin
Messenger RNA
Porosity
Cell proliferation
Scaffolds (biology)
RNA
DNA Replication
Osteogenesis
Gene expression
Cell Communication

Keywords

  • chitosan nanofibers
  • osteoblast maturation
  • osteoblast-associated gene expression
  • Runx2

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation. / Ho, Ming Hua; Liao, Mei Hsiu; Lin, Yi Ling; Lai, Chien Hao; Lin, Pei I.; Chen, Ruei Ming.

In: International Journal of Nanomedicine, Vol. 9, 2014, p. 4293-4304.

Research output: Contribution to journalArticle

Ho, Ming Hua ; Liao, Mei Hsiu ; Lin, Yi Ling ; Lai, Chien Hao ; Lin, Pei I. ; Chen, Ruei Ming. / Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation. In: International Journal of Nanomedicine. 2014 ; Vol. 9. pp. 4293-4304.
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