Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence

Chih Yu Wang, Tsuimin Tsai, Chung Ping Chiang, Hsin Ming Chen, Chin Tin Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We investigate the possibility of using ALA-derived PpIX fluorescence spectroscopy for the detection of epithelial hyperkeratosis (EH) or epithelial dysplasia (ED) lesions in oral submucous fibrosis (OSF) patients that could not be found by autofluorescence spectroscopy. Twenty percent of ALA solution gel was applied onto oral neoplasia and surrounding normal tissue [normal oral mucosa (NOM)] for 90min. Fluorescence emission spectra were measured under 410nm excitation. Generally, the most intense fluorescence emission peaks occurred at 460 and 630nm. The ratios of the area under red peak (630±10nm) to the area under blue peak (460±10nm), denoted as R/B, were calculated. We found that OSF mucosa has the lowest R/B value, followed by NOM, EH on OSF, and ED on OSF. An ANOVA test showed significant differences between OSF, NOM, EH on OSF, and ED on OSF (p0.05). These results indicate that ALA-induced PpIX fluorescence spectroscopy could be used to identify the premalignant lesions on oral fibrotic mucosa, which could not be found by autofluorescence.

Original languageEnglish
Article number044026
JournalJournal of Biomedical Optics
Volume14
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

fibrosis
Aminolevulinic Acid
lesions
Fluorescence
fluorescence
acids
Acids
Fluorescence spectroscopy
Analysis of variance (ANOVA)
spectroscopy
Gels
Mucous Membrane
Spectroscopy
Tissue
emission spectra
gels
excitation

Keywords

  • 5-aminolevulinic acid
  • oral neoplasia
  • oral submucous fibrosis

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence. / Wang, Chih Yu; Tsai, Tsuimin; Chiang, Chung Ping; Chen, Hsin Ming; Chen, Chin Tin.

In: Journal of Biomedical Optics, Vol. 14, No. 4, 044026, 2009.

Research output: Contribution to journalArticle

Wang, Chih Yu ; Tsai, Tsuimin ; Chiang, Chung Ping ; Chen, Hsin Ming ; Chen, Chin Tin. / Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence. In: Journal of Biomedical Optics. 2009 ; Vol. 14, No. 4.
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