Implantable Graphene-based Neural Electrode Interfaces for Electrophysiology and Neurochemistry in In Vivo Hyperacute Stroke Model

Ta Chung Liu, Min Chieh Chuang, Chao Yi Chu, Wei Chen Huang, Hsin Yi Lai, Chao Ting Wang, Wei Lin Chu, San Yuan Chen, You Yin Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Implantable microelectrode arrays have attracted considerable interest due to their high temporal and spatial resolution recording of neuronal activity in tissues. We herein presented an implantable multichannel neural probe with multiple real-time monitoring of neural-chemical and neural-electrical signals by a nonenzymatic neural-chemical interface, which was designed by creating the newly developed reduced graphene oxide-gold oxide (rGO/Au2O3) nanocomposite electrode. The modified electrode on the neural probe was prepared by a facile one-step cyclic voltammetry (CV) electrochemical method with simultaneous occurrence of gold oxidation and GOs reduction to induce the intimate attachment by electrostatic interaction using chloride ions (Cl-). The rGO/Au2O3-modified electrode at a low deposition scan rate of 10 mVs-1 displayed significantly improved electrocatalytic activity due to large active areas and well-dispersive attached rGO sheets. The in vitro amperometric response to H2O2 demonstrated a fast response of less than 5 s and a very low detection limit of 0.63 M. In in vivo hyperacute stroke model, the concentration of H2O2 was measured as 100.48 ± 4.52 M for rGO/Au2O3 electrode within 1 h photothrombotic stroke, which was much higher than that (71.92 M ± 2.52 M) for noncoated electrode via in vitro calibration. Simultaneously, the somatosensory-evoked potentials (SSEPs) test provided reliable and precise validation for detecting functional changes of neuronal activities. This newly developed implantable probe with localized rGO/Au2O3 nanocomposite electrode can serve as a rapid and reliable sensing platform for practical H2O2 detection in the brain or for other neural-chemical molecules in vivo.

Original languageEnglish
Pages (from-to)187-196
Number of pages10
JournalACS Applied Materials and Interfaces
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 13 2016
Externally publishedYes

Fingerprint

Electrophysiology
Graphite
Graphene
Electrodes
Gold
Oxides
Nanocomposites
Microelectrodes
Bioelectric potentials
Coulomb interactions
Cyclic voltammetry
Neurochemistry
Chlorides
Brain
Calibration
Ions
Tissue
Oxidation
Molecules
Monitoring

Keywords

  • electrophysiology
  • multichannel neural probe
  • neurochemistry
  • rGO/AuO nanocomposite
  • stroke

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Implantable Graphene-based Neural Electrode Interfaces for Electrophysiology and Neurochemistry in In Vivo Hyperacute Stroke Model. / Liu, Ta Chung; Chuang, Min Chieh; Chu, Chao Yi; Huang, Wei Chen; Lai, Hsin Yi; Wang, Chao Ting; Chu, Wei Lin; Chen, San Yuan; Chen, You Yin.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 1, 13.01.2016, p. 187-196.

Research output: Contribution to journalArticle

Liu, Ta Chung ; Chuang, Min Chieh ; Chu, Chao Yi ; Huang, Wei Chen ; Lai, Hsin Yi ; Wang, Chao Ting ; Chu, Wei Lin ; Chen, San Yuan ; Chen, You Yin. / Implantable Graphene-based Neural Electrode Interfaces for Electrophysiology and Neurochemistry in In Vivo Hyperacute Stroke Model. In: ACS Applied Materials and Interfaces. 2016 ; Vol. 8, No. 1. pp. 187-196.
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