Impairment of gingival fibroblast adherence by IL-6/sIL-6R

K. Naruishi, S. Takashiba, F. Nishimura, H. H. Chou, H. Arai, H. Yamada, Y. Murayama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gp130 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-α5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of β-actin and integrin-α5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.

Original languageEnglish
Pages (from-to)1421-1424
Number of pages4
JournalJournal of Dental Research
Volume80
Issue number5
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Interleukin-6 Receptors
Integrin alpha5beta1
Interleukin-6
Fibroblasts
Actins
Integrins
Transcription Factors
Cytokine Receptor gp130
Messenger RNA
Genes
Proteins

Keywords

  • Actin
  • Adherence
  • Gingival fibroblast
  • IL-6/sIL-6R
  • VLA-5

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Naruishi, K., Takashiba, S., Nishimura, F., Chou, H. H., Arai, H., Yamada, H., & Murayama, Y. (2001). Impairment of gingival fibroblast adherence by IL-6/sIL-6R. Journal of Dental Research, 80(5), 1421-1424.

Impairment of gingival fibroblast adherence by IL-6/sIL-6R. / Naruishi, K.; Takashiba, S.; Nishimura, F.; Chou, H. H.; Arai, H.; Yamada, H.; Murayama, Y.

In: Journal of Dental Research, Vol. 80, No. 5, 2001, p. 1421-1424.

Research output: Contribution to journalArticle

Naruishi, K, Takashiba, S, Nishimura, F, Chou, HH, Arai, H, Yamada, H & Murayama, Y 2001, 'Impairment of gingival fibroblast adherence by IL-6/sIL-6R', Journal of Dental Research, vol. 80, no. 5, pp. 1421-1424.
Naruishi K, Takashiba S, Nishimura F, Chou HH, Arai H, Yamada H et al. Impairment of gingival fibroblast adherence by IL-6/sIL-6R. Journal of Dental Research. 2001;80(5):1421-1424.
Naruishi, K. ; Takashiba, S. ; Nishimura, F. ; Chou, H. H. ; Arai, H. ; Yamada, H. ; Murayama, Y. / Impairment of gingival fibroblast adherence by IL-6/sIL-6R. In: Journal of Dental Research. 2001 ; Vol. 80, No. 5. pp. 1421-1424.
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