Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model

Kuo Chuan Wang, Sung Chun Tang, Jing Er Lee, Jui Chang Tsai, Dar Ming Lai, Wei Chou Lin, Chih Peng Lin, Yong-Kwang Tu, Sung Tsang Hsieh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The influence of aneurysmal subarachnoid hemorrhage (SAH) on brain microcirculation has not yet been systematically investigated. We established an animal model to examine (1) the brain surface microcirculation (2) the influences of cerebrospinal fluid (CSF) from aneurysmal SAH on the brain surface microcirculation. A rat SAH model was induced by injection of autologous arterial blood into the cisterna magnum, and the brain surface microcirculation was evaluated by a capillary videoscope with craniotomy at the fronto-parietal region. CSF from SAH rats and SAH patients was applied on the brain surface of naïve rats to assess the resulting microcirculatory changes. In the SAH rats, diffuse constriction of cortical arterioles within 24 hours of SAH was observed. Similar patterns of microcirculation impairment were induced on normal rat brain surfaces via application of CSF from SAH rats and SAH patients. Furthermore, the proportion of subjects with arteriolar vasoconstriction was significantly higher in the group of SAH patients with delayed ischemic neurological deficits (DIND) than in those without DIND (p < 0.001). This study demonstrated impaired microcirculation on brain surface arterioles in a rat model of SAH. CSF from SAH rats and patients was responsible for impairment of brain surface microcirculation.

Original languageEnglish
Article number13315
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Subarachnoid Hemorrhage
Microcirculation
Animal Models
Brain
Cerebrospinal Fluid
Arterioles
Parietal Lobe
Craniotomy
Vasoconstriction
Constriction

ASJC Scopus subject areas

  • General

Cite this

Wang, K. C., Tang, S. C., Lee, J. E., Tsai, J. C., Lai, D. M., Lin, W. C., ... Hsieh, S. T. (2018). Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model. Scientific Reports, 8(1), [13315]. https://doi.org/10.1038/s41598-018-31709-7

Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model. / Wang, Kuo Chuan; Tang, Sung Chun; Lee, Jing Er; Tsai, Jui Chang; Lai, Dar Ming; Lin, Wei Chou; Lin, Chih Peng; Tu, Yong-Kwang; Hsieh, Sung Tsang.

In: Scientific Reports, Vol. 8, No. 1, 13315, 01.12.2018.

Research output: Contribution to journalArticle

Wang, KC, Tang, SC, Lee, JE, Tsai, JC, Lai, DM, Lin, WC, Lin, CP, Tu, Y-K & Hsieh, ST 2018, 'Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model', Scientific Reports, vol. 8, no. 1, 13315. https://doi.org/10.1038/s41598-018-31709-7
Wang, Kuo Chuan ; Tang, Sung Chun ; Lee, Jing Er ; Tsai, Jui Chang ; Lai, Dar Ming ; Lin, Wei Chou ; Lin, Chih Peng ; Tu, Yong-Kwang ; Hsieh, Sung Tsang. / Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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