Impacts of hypervirulence determinants on clinical features and outcomes of bacteremia caused by extended-spectrum β-lactamase-producing klebsiella pneumoniae

Wen Liang Yu, Mei Feng Lee, Chi Chung Chen, Hung Jen Tang, Chung Han Ho, Yin Ching Chuang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We investigated the implications of hypervirulence determinants on clinical features of 48 adult patients with bacteremia caused by extended-spectrum β-lactamase-producing Klebsiella pneumoniae. Isolates in the hypervirulence group included any of the following virulence determinants: K1/K2 capsule serotypes, hypermucoviscosity phenotype, rmpA gene, or rmpA2 gene. Nonhypervirulence group isolates were negative for all of the above virulence factors. In this study, all isolates used were non-K1/K2 strains. Statistically significant differences were observed in clinical features of patients between the two groups. The hypervirulent isolates (n = 19), including 11 isolates with the hypermucoviscosity phenotype, 15 with the rmpA gene, and 16 with the rmpA2 gene, were more commonly recovered from diabetic patients and mainly manifested as secondary bacteremia (such as pneumonia, urinary tract infections, or other localized infections). The nonhypervirulent isolates (n = 29) were more commonly recovered from patients after prolonged hospital stays (>30 days) and mostly manifested as primary bacteremia. The overall in-hospital mortality was 56.3%. Hazard ratio (HR) analysis revealed the following positive predictors for mortality: nosocomial infection, stay in an intensive care unit, no removal of the central venous catheter, Charlson comorbidity score, and APACHE II score (≥15). The negative predictors were initial appropriate antibiotic therapy (HR 0.42) and urinary tract infection (HR 0.19). Charlson score was an independent confounder based on multivariate analysis (HR 1.43, 95% confidence interval 1.04-1.99). In conclusion, hypervirulence determinants played a role in causing secondary infections in diabetic patients; however, the presence of morbidity cofactors could themselves influence mortality, despite the absence of hypervirulence determinants.

Original languageEnglish
Pages (from-to)376-383
Number of pages8
JournalMicrobial Drug Resistance
Volume23
Issue number3
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Klebsiella pneumoniae
Bacteremia
Urinary Tract Infections
Genes
Phenotype
APACHE
Central Venous Catheters
Mortality
Virulence Factors
Cross Infection
Hospital Mortality
Coinfection
Capsules
Intensive Care Units
Virulence
Comorbidity
Length of Stay
Pneumonia
Multivariate Analysis
Confidence Intervals

Keywords

  • ESBL
  • hypermucoviscosity
  • hypervirulence
  • Klebsiella pneumoniae
  • rmpA
  • virulence

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

Cite this

Impacts of hypervirulence determinants on clinical features and outcomes of bacteremia caused by extended-spectrum β-lactamase-producing klebsiella pneumoniae. / Yu, Wen Liang; Lee, Mei Feng; Chen, Chi Chung; Tang, Hung Jen; Ho, Chung Han; Chuang, Yin Ching.

In: Microbial Drug Resistance, Vol. 23, No. 3, 01.04.2017, p. 376-383.

Research output: Contribution to journalArticle

Yu, Wen Liang ; Lee, Mei Feng ; Chen, Chi Chung ; Tang, Hung Jen ; Ho, Chung Han ; Chuang, Yin Ching. / Impacts of hypervirulence determinants on clinical features and outcomes of bacteremia caused by extended-spectrum β-lactamase-producing klebsiella pneumoniae. In: Microbial Drug Resistance. 2017 ; Vol. 23, No. 3. pp. 376-383.
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