Impact of microRNA-34a and polymorphism of its target gene CA9 on susceptibility to uterine cervical cancer

Shun Fa Yang, Yu Fan Liu, Chao Wen Cheng, Wei En Yang, Wea Lung Lin, Jiunn Liang Ko, Po Hui Wang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The purposes of this study were to associate the genetic polymorphisms in carbonic anhydrase (CA) 9 with uterine cervical cancer and identify the clinical implications. Three single-nucleotide polymorphisms (SNPs), rs2071676 (+201, G/A), rs3829078 (+1081, A/G), and rs1048638 (+1584, C/A), and an 18-base-pair deletion/insertion (376del393) in CA9 were examined. We used the Boyden chamber assay to evaluate the influence of CA9 on the migration of cervical cancers. Tissue microarrays were used to evaluate CAIX immunoreactivity and determine its clinical significance. The results revealed that the CA9 SNP rs1048638 is the only significant polymorphism that increases the risk of cervical cancer in Taiwanese women. We discovered that the CA9 SNP rs1048638 influences the expression of CA9 through the interaction between the 3'-untranslated region (UTR) of exon 11, where the SNP is located, and miR-34a, and influences the migration of cervical cancer cells. Moreover, we demonstrated that CAIX immunoreactivity is related to the occurrence of cervical cancer, and elevated CAIX immunoreactivity is associated with a more advanced stage. In conclusion, the finding that the CA9 SNP rs1048638 exerts its action through duplexes of the miR-34a and CA9 3'-UTRs and plays a vital role in cervical cancer in Taiwanese women may be applicable to translational medicine.

Original languageEnglish
Pages (from-to)77860-77871
Number of pages12
JournalOncotarget
Volume8
Issue number44
DOIs
Publication statusPublished - 2017

Keywords

  • Carbonic anhydrase
  • Migration
  • MiR-34a
  • Single nucleotide polymorphisms
  • Tissue microarray

ASJC Scopus subject areas

  • Oncology

Fingerprint Dive into the research topics of 'Impact of microRNA-34a and polymorphism of its target gene CA9 on susceptibility to uterine cervical cancer'. Together they form a unique fingerprint.

Cite this