Immunostimulatory activities of yam tuber mucilages

Huey Fang Shang, Huey Chuan Cheng, Hong Jen Liang, Hao Yu Liu, Sin Yie Liu, Wen Chi Hou

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The purified mucilages from three Taiwanese yam cultivars, including Dioscorea alata L. cv. Tainong 1 (TN1), D. alata L. cv. Tainong 2 (TN2), and D. alata L. var. purpurea (Roxb.) cv. Ming-Jen (MJ), and the commercial lentinan (Len) were used to evaluate the immunostimulatory effects on the murine innate and adaptive immunity. BALB/c mice were grouped and administrated orally with 0.5 ml of TN1, TN2, MJ daily for 5 weeks. The positive and negative controls were fed with lentinan and distilled water, respectively. Blood samples were drawn from the retroorbital sinus on day 7 and 21, and the lymphocyte subpopulation, phagocytosis of granulocyte and monocyte were analyzed by flow cytometry. The mice were sacrificed on day 36, and the splenocytes were harvested for determinations of natural killer (NK) cell cytotoxicity activity. The stimulation index on the phagocytosis of peritoneal macrophages and the RAW264.7 cell line by yam mucilage were also determined in vitro. The results showed that all three mucilages, especially MJ yam, could elevate the number of T helper cells in the peripheral blood and enhance the phagocytic activity of granulocyte, monocytes and macrophages both ex vivo and in vitro tests. Increased splenic cytotoxic activity following the administration of mucilages from MJ yam was observed. Furthermore, the production of specific anti-ovalbumin (OVA) antibody and OVA-stimulated splenic cell proliferation were also enhanced by all mucilage groups. It is suggested that the tuber mucilage may function as an immunomodulatory substance.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBotanical Studies
Volume48
Issue number1
Publication statusPublished - Jan 2007

Fingerprint

mucilages
yams
tubers
Dioscorea alata
phagocytosis
ovalbumin
granulocytes
monocytes
mice
macrophages
blood
natural killer cells
splenocytes
sinuses
flow cytometry
cytotoxicity
cell proliferation
lymphocytes
T-lymphocytes
cell lines

Keywords

  • Dioscorea
  • Immunostimulatory
  • Lentinan
  • Mucilage
  • Nk cell
  • Phagocytic activity
  • Yam

ASJC Scopus subject areas

  • Plant Science

Cite this

Shang, H. F., Cheng, H. C., Liang, H. J., Liu, H. Y., Liu, S. Y., & Hou, W. C. (2007). Immunostimulatory activities of yam tuber mucilages. Botanical Studies, 48(1), 63-70.

Immunostimulatory activities of yam tuber mucilages. / Shang, Huey Fang; Cheng, Huey Chuan; Liang, Hong Jen; Liu, Hao Yu; Liu, Sin Yie; Hou, Wen Chi.

In: Botanical Studies, Vol. 48, No. 1, 01.2007, p. 63-70.

Research output: Contribution to journalArticle

Shang, HF, Cheng, HC, Liang, HJ, Liu, HY, Liu, SY & Hou, WC 2007, 'Immunostimulatory activities of yam tuber mucilages', Botanical Studies, vol. 48, no. 1, pp. 63-70.
Shang HF, Cheng HC, Liang HJ, Liu HY, Liu SY, Hou WC. Immunostimulatory activities of yam tuber mucilages. Botanical Studies. 2007 Jan;48(1):63-70.
Shang, Huey Fang ; Cheng, Huey Chuan ; Liang, Hong Jen ; Liu, Hao Yu ; Liu, Sin Yie ; Hou, Wen Chi. / Immunostimulatory activities of yam tuber mucilages. In: Botanical Studies. 2007 ; Vol. 48, No. 1. pp. 63-70.
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