Immunoprophylaxis of allergen-induced immunoglobulin E synthesis and airway hyperresponsiveness in vivo by genetic immunization

Ching Hsiang Hsu, Kaw Yan Chua, M. I.Hua Tao, Yih Loong Lai, Heuy Dong Wu, Shau Ku Huang, Kue Hsiung Hsieh

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

The efficacy of an 'allergen-gene immunization' protocol in altering allergic response was examined. Intramuscular injection of rats with a plasmid DNA encoding a house dust mite allergen into the muscle results in its long-term expression and the induction of specific immune responses. Significantly, this approach prevents the induction of immunoglobulin E synthesis, histamine release in bronchoalveolar fluids, and airway hyperresponsiveness in rats challenged with aerosolized allergen. Furthermore, this suppression is persistent and can be transferred into naive rats by CD8+ T cells from gene-immunized rats. These findings suggest that allergen-gene immunization is effective in modulating allergic responses, and may provide a novel therapeutic approach for allergic diseases.

Original languageEnglish
Pages (from-to)540-544
Number of pages5
JournalNature Medicine
Volume2
Issue number5
DOIs
Publication statusPublished - May 23 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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