Immunophenotypic and genotypic characteristics of chronic myelogenous leukemia in blast crisis

C. C. Yen, J. H. Liu, W. S. Wang, F. S. Fan, T. J. Chiou, C. J. Tai, M. H. Yang, T. C. Chao, L. T. Hsiao, P. M. Chen

Research output: Contribution to journalArticle

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Abstract

Background. Chronic myelogenous leukemia (CML) may transform into blast crisis (BC) if not properly treated. Among patients with transformation, 20% to 30% will develop BC with lymphoid-associated antigens (Ly-BC), and the remaining cases with myeloid-associated antigens (My-BC) or with both (Mix-BC). In this study, we investigated the lineage of blast cells in CML-BC using immunophenotypic and genetic analyses and analyzed the prognostic significance of genotypic change in CML-BC. Methods. Twenty-one patients with CML-BC diagnosed at the Taipei Veterans General Hospital from 1982 to 1992 were included. Immunophenotyping was done by using the avidin-biotin immunoperoxidase technique. Genetic analyses were carried out by using Southern Blot hybridization. The prognostic influence of genotypic change was analyzed. Results. Thirteen patients (61.9%) expressed myeloid-associated antigens, one patient (4.8%) expressed megakaryoblast-associated antigens, four patients (19%) expressed B lymphoid-associated antigens and three patients (14.3 %) expressed both myeloid and B lymphoid antigens. Clonal rearrangement of the immunoglobulin heavy chain (IgH) gene was found in six cases. Among them, four expressed B lymphoid markers only and two expressed both myeloid and B lymphoid markers. Patients with clonal IgH gene rearrangement tended to have a better response to chemotherapy (50% vs 8.3%, p = 0.08) and significantly longer survival (median survival, 5 months vs 3 months, p <0.05) than did those with a germline configuration. Conclusions. Clonal rearrangement of the IgH gene was found mostly in cases of Ly-BC and Mix-BC. We found that CML-BC with clonal rearrangement of the IgH gene had a more favorable prognosis than in cases with a germline configuration.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalChinese Medical Journal (Taipei)
Volume63
Issue number11
Publication statusPublished - 2000
Externally publishedYes

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Blast Crisis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Immunoglobulin Heavy Chain Genes
Antigens
Ly Antigens
Megakaryocyte Progenitor Cells
Veterans Hospitals
Immunophenotyping
Survival
Avidin
Gene Rearrangement
Biotin
Southern Blotting
Immunoenzyme Techniques
General Hospitals

Keywords

  • Chronic myeloid leukemia
  • Gene rearrangement
  • Immunophenotyping

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yen, C. C., Liu, J. H., Wang, W. S., Fan, F. S., Chiou, T. J., Tai, C. J., ... Chen, P. M. (2000). Immunophenotypic and genotypic characteristics of chronic myelogenous leukemia in blast crisis. Chinese Medical Journal (Taipei), 63(11), 785-791.

Immunophenotypic and genotypic characteristics of chronic myelogenous leukemia in blast crisis. / Yen, C. C.; Liu, J. H.; Wang, W. S.; Fan, F. S.; Chiou, T. J.; Tai, C. J.; Yang, M. H.; Chao, T. C.; Hsiao, L. T.; Chen, P. M.

In: Chinese Medical Journal (Taipei), Vol. 63, No. 11, 2000, p. 785-791.

Research output: Contribution to journalArticle

Yen, CC, Liu, JH, Wang, WS, Fan, FS, Chiou, TJ, Tai, CJ, Yang, MH, Chao, TC, Hsiao, LT & Chen, PM 2000, 'Immunophenotypic and genotypic characteristics of chronic myelogenous leukemia in blast crisis', Chinese Medical Journal (Taipei), vol. 63, no. 11, pp. 785-791.
Yen, C. C. ; Liu, J. H. ; Wang, W. S. ; Fan, F. S. ; Chiou, T. J. ; Tai, C. J. ; Yang, M. H. ; Chao, T. C. ; Hsiao, L. T. ; Chen, P. M. / Immunophenotypic and genotypic characteristics of chronic myelogenous leukemia in blast crisis. In: Chinese Medical Journal (Taipei). 2000 ; Vol. 63, No. 11. pp. 785-791.
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abstract = "Background. Chronic myelogenous leukemia (CML) may transform into blast crisis (BC) if not properly treated. Among patients with transformation, 20{\%} to 30{\%} will develop BC with lymphoid-associated antigens (Ly-BC), and the remaining cases with myeloid-associated antigens (My-BC) or with both (Mix-BC). In this study, we investigated the lineage of blast cells in CML-BC using immunophenotypic and genetic analyses and analyzed the prognostic significance of genotypic change in CML-BC. Methods. Twenty-one patients with CML-BC diagnosed at the Taipei Veterans General Hospital from 1982 to 1992 were included. Immunophenotyping was done by using the avidin-biotin immunoperoxidase technique. Genetic analyses were carried out by using Southern Blot hybridization. The prognostic influence of genotypic change was analyzed. Results. Thirteen patients (61.9{\%}) expressed myeloid-associated antigens, one patient (4.8{\%}) expressed megakaryoblast-associated antigens, four patients (19{\%}) expressed B lymphoid-associated antigens and three patients (14.3 {\%}) expressed both myeloid and B lymphoid antigens. Clonal rearrangement of the immunoglobulin heavy chain (IgH) gene was found in six cases. Among them, four expressed B lymphoid markers only and two expressed both myeloid and B lymphoid markers. Patients with clonal IgH gene rearrangement tended to have a better response to chemotherapy (50{\%} vs 8.3{\%}, p = 0.08) and significantly longer survival (median survival, 5 months vs 3 months, p <0.05) than did those with a germline configuration. Conclusions. Clonal rearrangement of the IgH gene was found mostly in cases of Ly-BC and Mix-BC. We found that CML-BC with clonal rearrangement of the IgH gene had a more favorable prognosis than in cases with a germline configuration.",
keywords = "Chronic myeloid leukemia, Gene rearrangement, Immunophenotyping",
author = "Yen, {C. C.} and Liu, {J. H.} and Wang, {W. S.} and Fan, {F. S.} and Chiou, {T. J.} and Tai, {C. J.} and Yang, {M. H.} and Chao, {T. C.} and Hsiao, {L. T.} and Chen, {P. M.}",
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T1 - Immunophenotypic and genotypic characteristics of chronic myelogenous leukemia in blast crisis

AU - Yen, C. C.

AU - Liu, J. H.

AU - Wang, W. S.

AU - Fan, F. S.

AU - Chiou, T. J.

AU - Tai, C. J.

AU - Yang, M. H.

AU - Chao, T. C.

AU - Hsiao, L. T.

AU - Chen, P. M.

PY - 2000

Y1 - 2000

N2 - Background. Chronic myelogenous leukemia (CML) may transform into blast crisis (BC) if not properly treated. Among patients with transformation, 20% to 30% will develop BC with lymphoid-associated antigens (Ly-BC), and the remaining cases with myeloid-associated antigens (My-BC) or with both (Mix-BC). In this study, we investigated the lineage of blast cells in CML-BC using immunophenotypic and genetic analyses and analyzed the prognostic significance of genotypic change in CML-BC. Methods. Twenty-one patients with CML-BC diagnosed at the Taipei Veterans General Hospital from 1982 to 1992 were included. Immunophenotyping was done by using the avidin-biotin immunoperoxidase technique. Genetic analyses were carried out by using Southern Blot hybridization. The prognostic influence of genotypic change was analyzed. Results. Thirteen patients (61.9%) expressed myeloid-associated antigens, one patient (4.8%) expressed megakaryoblast-associated antigens, four patients (19%) expressed B lymphoid-associated antigens and three patients (14.3 %) expressed both myeloid and B lymphoid antigens. Clonal rearrangement of the immunoglobulin heavy chain (IgH) gene was found in six cases. Among them, four expressed B lymphoid markers only and two expressed both myeloid and B lymphoid markers. Patients with clonal IgH gene rearrangement tended to have a better response to chemotherapy (50% vs 8.3%, p = 0.08) and significantly longer survival (median survival, 5 months vs 3 months, p <0.05) than did those with a germline configuration. Conclusions. Clonal rearrangement of the IgH gene was found mostly in cases of Ly-BC and Mix-BC. We found that CML-BC with clonal rearrangement of the IgH gene had a more favorable prognosis than in cases with a germline configuration.

AB - Background. Chronic myelogenous leukemia (CML) may transform into blast crisis (BC) if not properly treated. Among patients with transformation, 20% to 30% will develop BC with lymphoid-associated antigens (Ly-BC), and the remaining cases with myeloid-associated antigens (My-BC) or with both (Mix-BC). In this study, we investigated the lineage of blast cells in CML-BC using immunophenotypic and genetic analyses and analyzed the prognostic significance of genotypic change in CML-BC. Methods. Twenty-one patients with CML-BC diagnosed at the Taipei Veterans General Hospital from 1982 to 1992 were included. Immunophenotyping was done by using the avidin-biotin immunoperoxidase technique. Genetic analyses were carried out by using Southern Blot hybridization. The prognostic influence of genotypic change was analyzed. Results. Thirteen patients (61.9%) expressed myeloid-associated antigens, one patient (4.8%) expressed megakaryoblast-associated antigens, four patients (19%) expressed B lymphoid-associated antigens and three patients (14.3 %) expressed both myeloid and B lymphoid antigens. Clonal rearrangement of the immunoglobulin heavy chain (IgH) gene was found in six cases. Among them, four expressed B lymphoid markers only and two expressed both myeloid and B lymphoid markers. Patients with clonal IgH gene rearrangement tended to have a better response to chemotherapy (50% vs 8.3%, p = 0.08) and significantly longer survival (median survival, 5 months vs 3 months, p <0.05) than did those with a germline configuration. Conclusions. Clonal rearrangement of the IgH gene was found mostly in cases of Ly-BC and Mix-BC. We found that CML-BC with clonal rearrangement of the IgH gene had a more favorable prognosis than in cases with a germline configuration.

KW - Chronic myeloid leukemia

KW - Gene rearrangement

KW - Immunophenotyping

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VL - 63

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JO - Journal of the Chinese Medical Association

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