Abstract

Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity. Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.

Original languageEnglish
Pages (from-to)14862-14875
Number of pages14
JournalMolecules
Volume18
Issue number12
DOIs
Publication statusPublished - Dec 2013

Fingerprint

leukopenia
macrophages
Macrophages
Leukopenia
mice
acetates
cells
Qi
Plant Preparations
leukocytes
Herbal Medicine
Scavenging
scavenging
Cytotoxicity
activity (biology)
medicine
Dinoprostone
markers
Single Nucleotide Polymorphism
Methanol

Keywords

  • Anti-inflammation
  • Astragali radix
  • Daunoblastina-induced leucopenia mice
  • Fabaceae
  • Hedysarum plybotrys handel-mazzetti (hedysari radix)
  • Splenocyte proliferation

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Immunomodulatory effects of hedysarum polybotrys extract in mice macrophages, splenocytes and leucopenia. / Huang, Guan Cheng; Lee, Chia Jung; Wang, Kun Teng; Weng, Bor Chun; Chien, Ting Yi; Tseng, Sung Hui; Wang, Ching Chiung.

In: Molecules, Vol. 18, No. 12, 12.2013, p. 14862-14875.

Research output: Contribution to journalArticle

Huang, Guan Cheng ; Lee, Chia Jung ; Wang, Kun Teng ; Weng, Bor Chun ; Chien, Ting Yi ; Tseng, Sung Hui ; Wang, Ching Chiung. / Immunomodulatory effects of hedysarum polybotrys extract in mice macrophages, splenocytes and leucopenia. In: Molecules. 2013 ; Vol. 18, No. 12. pp. 14862-14875.
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