Immunomodulating activity of lactobacillus paracasei subsp. paracasei NTU 101 in enterohemorrhagic escherichia coli O157H7-infected mice

Yueh Ting Tsai, Po Ching Cheng, Tzu Ming Pan

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The present study investigated the immunomodulating activity of Lactobacillus paracasei subsp. paracasei NTU 101 in enterohemorrhagic Escherichia coli O157:H7-infected BALB/c mice. Mice were given L. paracasei subsp. paracasei NTU 101 (108 colony-forming units) for 7 days, before and after the challenge with E. coli O157:H7. Feeding Lactobacillus for 7 days resulted in an increased postchallenge weight gain and lower cumulative morbidity rates. We observed the upregulation of dendritic cells, helper T cell activation, and antibody production in post-and pretreated mice, compared with untreated mice in the E. coli O157:H7 infection group. Moreover, Lactobacillus can down-regulate the expression of toll-like receptors (TLRs) on macrophages and proinflammatory cytokines, and chemokines in the post-or prefeeding mice induce by E. coli O157:H7 infection. These results demonstrated the inhibition of inflammation among the mice in the pretreated group than in the post-treated group by modulating their immune response. These findings suggest that L. paracasei subsp. paracasei NTU 101 may be an effective candidate for use as a probiotic in the prevention of infection caused by E. coli O157:H7 in humans.

Original languageEnglish
Pages (from-to)11265-11272
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number21
DOIs
Publication statusPublished - Nov 10 2010
Externally publishedYes

Keywords

  • Escherichia coli O157:H7
  • immunomodulation
  • probiotics
  • Toll-like receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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