Immunohistochemical study of amoeboid microglial cells in fetal rat brain

C. C. Wang, C. H. Wu, J. Y. Shieh, C. Y. Wen, E. A. Ling

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The present study examined the expression of different antigens in amoeboid microglial cells (AMC) in fetal rat brain extending from 12 to 20 d postconception (E12-E20) using a panel of monoclonal antibodies which recognised the major histocompatibility complex (MHC) class I (OX-18) and class II (OX-6) antigens, leucocyte common antigen (OX-1), CD4 receptor (OX-35), complement type 3 receptor (OX-42) or macrophage antigens of unknown function (ED1 and ED2). Of the above-mentioned antigens, ED1 and ED2-labelled AMC were observed in the neuroepithelia as early as embryonic day 12 (E12); other antigens were not detected at this stage. At E14, except for MHC class I antigen, all other antigens were expressed by AMC distributed predominantly in the developing white matter. At E16, AMC in the intermediate zone lateral to the striatum were endowed with all the above-mentioned antigens including MHC class I. At E18, the immunoreactivities of AMC stained with OX-6, OX-18, OX-35 and OX-42 antigens were noticeably reduced when compared with those cells at E16. At E20, amoeboid microglial cells exhibited full complement of antigen expression similar to those cells at E16; some of the labelled cells emitted a variable number of cytoplasmic processes. It is suggested that the successive and differential expression of various macrophage related antigens on AMC in fetal brain is related to the specific requirement of local environment in different stages of development.

Original languageEnglish
Pages (from-to)567-574
Number of pages8
JournalJournal of Anatomy
Volume189
Issue number3
Publication statusPublished - 1996

Fingerprint

antigen
brain
antigens
Antigens
rats
Brain
cells
major histocompatibility complex
Major Histocompatibility Complex
Macrophages
Macrophage-1 Antigen
CD45 Antigens
complement
macrophages
CD4 Antigens
Histocompatibility Antigens Class I
receptors
antibody
Monoclonal Antibodies
leukocytes

Keywords

  • Antigen expression
  • Glia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Wang, C. C., Wu, C. H., Shieh, J. Y., Wen, C. Y., & Ling, E. A. (1996). Immunohistochemical study of amoeboid microglial cells in fetal rat brain. Journal of Anatomy, 189(3), 567-574.

Immunohistochemical study of amoeboid microglial cells in fetal rat brain. / Wang, C. C.; Wu, C. H.; Shieh, J. Y.; Wen, C. Y.; Ling, E. A.

In: Journal of Anatomy, Vol. 189, No. 3, 1996, p. 567-574.

Research output: Contribution to journalArticle

Wang, CC, Wu, CH, Shieh, JY, Wen, CY & Ling, EA 1996, 'Immunohistochemical study of amoeboid microglial cells in fetal rat brain', Journal of Anatomy, vol. 189, no. 3, pp. 567-574.
Wang, C. C. ; Wu, C. H. ; Shieh, J. Y. ; Wen, C. Y. ; Ling, E. A. / Immunohistochemical study of amoeboid microglial cells in fetal rat brain. In: Journal of Anatomy. 1996 ; Vol. 189, No. 3. pp. 567-574.
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