Immunohistochemical localization of manganese superoxide dismutase in the rat cochlea

M. T. Lai, T. Ohmichi, K. Egusa, S. Okada, Y. Masuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

There has been recent increasing interest in the involvement of superoxide radicals (O2-) and their scavenging enzymes, the superoxide dismutases, in the pathophysiology of certain diseases. Since mitochondria are significant intracellular sources of O2- and important targets of oxidant injury, determining the intracochlear localization of mitochondrial O2- scavenging enzyme may provide important insight into the pathogenesis of injury due to cochlear oxidants. In order to locate the mitochondrial O2- scavenging enzyme, manganese superoxide dismutase (MnSOD), the authors used a modified immunoglobulin peroxidase bridge sequence method to detect MnSOD in paraffin-embedded, formalin-fixed rat cochleas. Site-specific immunolocalization of MnSOD could be demonstrated in the cochlear labyrinth, suggesting that the generation of intracochlear O2- was possibly implicated in the metabolically active sites and sites rich in vascularity. This study also provided a useful probe for detecting MnSOD immunohistochemically from ethylenediamine tetraacetic acid-treated materials without requiring an antigen retrieval procedure.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume253
Issue number4-5
Publication statusPublished - 1996

Fingerprint

Cochlea
Superoxide Dismutase
ethylenediamine
Oxidants
Enzymes
Wounds and Injuries
Inner Ear
Superoxides
Paraffin
Formaldehyde
Peroxidase
Immunoglobulins
Catalytic Domain
Mitochondria
Antigens
Acids

Keywords

  • Cochlear labyrinth
  • Immunohistochemical method
  • Manganese superoxide dismutase
  • Superoxide radicals

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Immunohistochemical localization of manganese superoxide dismutase in the rat cochlea. / Lai, M. T.; Ohmichi, T.; Egusa, K.; Okada, S.; Masuda, Y.

In: European Archives of Oto-Rhino-Laryngology, Vol. 253, No. 4-5, 1996, p. 273-277.

Research output: Contribution to journalArticle

Lai, M. T. ; Ohmichi, T. ; Egusa, K. ; Okada, S. ; Masuda, Y. / Immunohistochemical localization of manganese superoxide dismutase in the rat cochlea. In: European Archives of Oto-Rhino-Laryngology. 1996 ; Vol. 253, No. 4-5. pp. 273-277.
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