Immunohistochemical localization of manganese superoxide dismutase in the rat cochlea

M. T. Lai, T. Ohmichi, K. Egusa, S. Okada, Y. Masuda

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5 Citations (Scopus)

Abstract

There has been recent increasing interest in the involvement of superoxide radicals (O2-) and their scavenging enzymes, the superoxide dismutases, in the pathophysiology of certain diseases. Since mitochondria are significant intracellular sources of O2- and important targets of oxidant injury, determining the intracochlear localization of mitochondrial O2- scavenging enzyme may provide important insight into the pathogenesis of injury due to cochlear oxidants. In order to locate the mitochondrial O2- scavenging enzyme, manganese superoxide dismutase (MnSOD), the authors used a modified immunoglobulin peroxidase bridge sequence method to detect MnSOD in paraffin-embedded, formalin-fixed rat cochleas. Site-specific immunolocalization of MnSOD could be demonstrated in the cochlear labyrinth, suggesting that the generation of intracochlear O2- was possibly implicated in the metabolically active sites and sites rich in vascularity. This study also provided a useful probe for detecting MnSOD immunohistochemically from ethylenediamine tetraacetic acid-treated materials without requiring an antigen retrieval procedure.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume253
Issue number4-5
Publication statusPublished - 1996

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Keywords

  • Cochlear labyrinth
  • Immunohistochemical method
  • Manganese superoxide dismutase
  • Superoxide radicals

ASJC Scopus subject areas

  • Otorhinolaryngology

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